Culture type shapes drug‐induced growth arrest in ovarian cancer patient cells Culture type shapes drug‐induced growth arrest in ovarian cancer patient.

Slides:

Advertisements

Similar presentations

GFP‐Sed5p localization defect in sgt2Δ.

Advertisements

DNase‐HS sites are main independent determinants of DNA replication timing Simulations based on genome sequence features (GC content, CpG islands), or.

Cell death following DNA damage occurs from cells arrested in G1 or at the G1/S boundary. Cell death following DNA damage occurs from cells arrested in.

Abundance of proteins matching selected subcellular locations and functions in CaCo‐2 cells. Abundance of proteins matching selected subcellular locations.

CRISPR knockdown of the mrdAB operon increases cell width at high repression strengths CRISPR knockdown of the mrdAB operon increases cell width at high.

Overview of the experimental setting.

Single‐molecule tracking in living cells.

Hnf4g importance in human colon cancer organoids and regulation of the Hnf4a locus Hnf4g importance in human colon cancer organoids and regulation of the.

The HDAC3 inhibitor suppresses SPOP‐mutated prostate cancer cell growth The HDAC3 inhibitor suppresses SPOP‐mutated prostate cancer cell growth A22Rv1.

Functional analysis of duplicate pair CIK1–VIK1 (A) Genetic interaction profile similarity. Functional analysis of duplicate pair CIK1–VIK1 (A) Genetic.

Validation of the CCE associations by mass spectrometry.

Figure 2 The association between CD8+ T‑cell density of the tumour

The TBON model. The TBON model. (A) Representative confocal images of E14Tg2A cells stained for Tcf3 (green), Nanog (red), Oct4 (magenta), and total β‐catenin.

Comparative analysis of RNA and protein profiles.

Prediction of IGHV status based on Factor 1 in the CLL data and validation on outlier cases on independent assays Prediction of IGHV status based on Factor.

Confirmation of pool data with isogenic culture

Luminex phosphorylation measurements are reproducible and have broad dynamic range Individual difference in insulin‐induced phosphorylation of ERK measured.

Correlating protein to mRNA and proteins per mRNA ratios

Expression of FUT9 supports tumor development

Ovarian cancer cells harbour numerous copy number alterations unlinked to drug‐induced cell death Ovarian cancer cells harbour numerous copy number alterations.

Comparing histone profiles and DNA accessibility of secretory and enterocyte progenitor cells Comparing histone profiles and DNA accessibility of secretory.

Characterization of promoters relative to pAGA1

MiR‐199a directly targets ERBB2 and ERBB3, whereas miR‐125b targets ERBB3. miR‐199a directly targets ERBB2 and ERBB3, whereas miR‐125b targets ERBB3. (A)

Dynamics and variability of SMAD2 signaling in single cells

Genomic profiling of fitness in periodic salt stress

Expression of PD‐1 on PBMC and malignant exudate T cells

T22‐GFP‐H6‐FdU prevents metastasis in the M5 patient‐derived model in a CXCR4‐dependent manner T22‐GFP‐H6‐FdU prevents metastasis in the M5 patient‐derived.

Dermal architecture is defined by an inverse correlation between fibroblast proliferation and ECM deposition Dermal architecture is defined by an inverse.

Physical EGFR interactome generation and core network proteins identification. Physical EGFR interactome generation and core network proteins identification.

Homologous recombination deficiency scores correlate with drug‐induced cell death in primary ovarian cancer cells Homologous recombination deficiency scores.

BETi potentiate the efficacy of MEKi in NRAS‐mutant melanoma

Sensor optimization for thiosulfate and tetrathionate detection in the gut Sensor optimization for thiosulfate and tetrathionate detection in the gut A–F(A.

Changes to the growth conditions break the circuit by changing host gene expression Changes to the growth conditions break the circuit by changing host.

Experimental approach and reproducibility

Examples for intrachromosomal mRNA co‐regulation patches

Lysozyme expression in isolated Paneth cells and linage tracing of Notch1+ cells Lysozyme expression in isolated Paneth cells and linage tracing of Notch1+

Patient organoids respond more diverse to drugs and with lower therapeutic potential than 2D cultured patient cells Patient organoids respond more diverse.

Small‐molecule‐driven cell‐type enrichment as a model for intestinal differentiation Small‐molecule‐driven cell‐type enrichment as a model for intestinal.

Association of AIM2, RIG‐I and NLRP3 inflammasomes with better survival in NPC patients.A.Overexpression of ASC, caspase‐1 and IL‐1β proteins as well as.

DeathPro dyes Hoechst and PI do not affect organoid growth and drug responses DeathPro dyes Hoechst and PI do not affect organoid growth and drug responses.

Drug‐induced cell death and proliferation inhibition can be quantified from serial confocal images Drug‐induced cell death and proliferation inhibition.

Notch activation in Notch1 positive feedback knock‐out intestine organoids Notch activation in Notch1 positive feedback knock‐out intestine organoids Intestine.

Antisense transcription, measurement, and correction

Evaluation of assay performance with positive and negative binary reference sets Evaluation of assay performance with positive and negative binary reference.

Two‐way unsupervised uncentered unnormalized hierarchical clustering using a Pearson's correlation of the phenotypic profiles of 4281 nonessential genes.

Transcriptomic and epigenetic changes associated with Factor 1 in the scMT data Transcriptomic and epigenetic changes associated with Factor 1 in the scMT.

EnAd‐EpCAMBiTE shows reproducible activity within an expanded cohort of patient malignant peritoneal and pleural exudates EnAd‐EpCAMBiTE shows reproducible.

Effects of transcription factor silencing on transformed phenotypes expressed in KRAS‐transformed cells support hierarchical regulation in the gene‐regulatory.

Simulation showing that the cell length variability of the entire population can mask abnormal cell length variability at a specific cell cycle period.

In vivo efficacy of Olaparib in PTEN deficient xenografts.

An integrated NHR network.

Feedback loop signalling is necessary and sufficient to maintain proliferation arrest during establishment of irreversible cell senescence. Feedback loop.

Distinct collagen structures in the upper and lower neonatal dermis (related to Fig 1)‏ Distinct collagen structures in the upper and lower neonatal dermis.

Reproducibility of DeathPro drug screens

When grown with biofuel, strains with beneficial pumps dominate the culture. When grown with biofuel, strains with beneficial pumps dominate the culture.

Melting behavior of protein complexes

In vivo imaging of the wound bed at later time points (related to Fig 7)‏ In vivo imaging of the wound bed at later time points (related to Fig 7) A–CRepresentative.

Culture type determines effectivity of targeted drugs like SRC inhibitor dasatinib and mTOR inhibitors AZD2014 and temsirolimus Culture type determines.

Drug sensitivity described by LD50 is similar in cells cultured in 2D or as organoids Drug sensitivity described by LD50 is similar in cells cultured in.

Glucose pulses incorporate directly into the de novo biomass in non‐dividing cells Glucose pulses incorporate directly into the de novo biomass in non‐dividing.

Perturbation of ligand depletion affects the ultrasensitivity of long‐term P‐Smad2 responses to different doses of TGF‐β stimulation. Perturbation of ligand.

Glutamine addiction and cancer invasiveness are positively correlated in ovarian cancer cells (OVCA) Gln deprivation effect on proliferation rate of a.

Fraction of flux entering the PEP‐glyoxylate cycle as a function of hexose uptake rate in batch (A) and chemostat (B) cultures. Fraction of flux entering.

Validation of select PDN drug candidates in an in vivo endotoxemia model Validation of select PDN drug candidates in an in vivo endotoxemia model Therapeutic.

Bazedoxifene suppresses IL11‐mediated STAT3 signaling activity in human cancer cell lines and patient‐derived colon cancer primary cultures and organoids.

BRI1 signaling at the dividing cells restores overall root growth

PX-478 directly radiosensitizes tumor cells in vitro.

Antitumor effects of celastrol in vitro and in vivo.

ABT-888 sensitizes multiple ovarian cancer cell lines but not normal cells to FdUrd. ABT-888 sensitizes multiple ovarian cancer cell lines but not normal.

Interaction of cancer spheroids with mesothelium prompts mesothelial cell clearance. Interaction of cancer spheroids with mesothelium prompts mesothelial.

Presentation transcript:

Culture type shapes drug‐induced growth arrest in ovarian cancer patient cells Culture type shapes drug‐induced growth arrest in ovarian cancer patient cells Simplified overview of generation and cultivation of patient‐derived ovarian cancer cell lines (PDCLs) from different sites (OC: primary tumour, Asc: ascites, PE: pleural effusion). Patient material was taken directly into 2D culture (Asc211, PE306) or amplified by xenografting into mice. PDCLs are maintained in 2D culture but can be grown as ovarian cancer organoids on Matrigel.Morphology of ovarian cancer organoids and normal ovarian epithelial cells (HOSEpiC) on Matrigel 7 days after seeding. Green (Hoechst) and red (PI) channels are merged.Drug responses (cell death: AUCd, growth arrest: AUCpi) measured with DeathPro assay after 72‐h drug treatment in patient cells cultured as monolayers (2D) or ovarian cancer organoids (3D).Comparison of drug‐induced cell death (AUCd) and growth arrest (AUCpi) in 2D vs. 3D.Drug responses measured in ovarian cancer organoids (3D) after 72‐h drug treatment followed by 72‐h drug removal.Comparison of drug‐induced cell death and growth arrest in 2D vs. 3D after drug removal.Data information: All values shown are means of two independent biological replicates. HOSE, HOSEpiC; Rp, Pearson correlation coefficient; C + P, carboplatin + paclitaxel. Julia Jabs et al. Mol Syst Biol 2017;13:955 © as stated in the article, figure or figure legend