Systemic inflammation activates the exosomal machinery in the choroid plexus Systemic inflammation activates the exosomal machinery in the choroid plexus.

Slides:

Advertisements

Similar presentations

Effects of inhibiting inflammatory cell recruitment on fracture healing Treatment with CCR2 antagonist, INCB3344, led to impaired fracture healing compared.

Advertisements

Role of TNF in fracture healing in vivo Addition of rhTNF at the fracture site on days 0 and 1 led to augmented healing, indicated by increased % callus.

Induction of mPer1 mRNA expression by prostaglandin E2 (PGE2) in NIH3T3 cells. Induction of mPer1 mRNA expression by prostaglandin E2 (PGE2) in NIH3T3.

Reduced scar formation in the injured GM of CCR2−/− mice

Metabolic effects of TUG‐891 are reduced or absent in GPR120‐deficient mice Metabolic effects of TUG‐891 are reduced or absent in GPR120‐deficient mice.

Trx80 is generated by α‐secretases. A

The HDAC3 inhibitor suppresses SPOP‐mutated prostate cancer cell growth The HDAC3 inhibitor suppresses SPOP‐mutated prostate cancer cell growth A22Rv1.

AβOs bind to and impact hypothalamic neurons Representative immunocytochemistry images of mature hypothalamic neurons in culture exposed to vehicle (Veh)

TUG‐891 increases oxygen consumption by brown adipocytes, mediated by direct UCP1 activation and mitochondrial fragmentation TUG‐891 increases oxygen consumption.

The gene encoding TP53INP1 is expressed in pancreatic endocrine cells A, B(A, B) Immunocytofluorescent staining of TP53INP1 (red) and insulin (green) in.

PbA‐specific CD4 T‐cell responses are maximal at day 6 post‐pRBC infection PbA‐specific CD4 T‐cell responses are maximal at day 6 post‐pRBC infection Proportion.

Treatment with p38γ/p38δ inhibitor shows antifungal effects in vivo

Klotho reduction causes macrophage invasion into the CP

Mitochondrial targeting of ceramide induces lethal mitophagy in HPV(+) cells Mitochondrial targeting of ceramide induces lethal mitophagy in HPV(+) cells.

Treatment with FGF21‐encoding vectors reverses hepatic steatosis and fibrosis Treatment with FGF21‐encoding vectors reverses hepatic steatosis and fibrosis.

Engagement of CD1d on ILC3s induces IL‐22 production

TNFR1 deficiency protects against morphological alterations in the choroid plexus of APP/PS1tg/wt mice determined by TEM TNFR1 deficiency protects against.

Metabolic profile of Tfe3 KO mice

Both haematopoietic and non‐haematopoietic compartments are involved in myocarditis development.A.Kaplan‐Meier survival curves showing survival of bone.

RFGF21 induces neurite outgrowth in GnRH neurons in vitro, stimulates GnRH secretion ex vivo, and reaches GnRH neurons by fenestrated vessels in vivo rFGF21.

TGF‐β1 and Sema3A are downregulated in vivo by increasing VEGF doses Muscles were harvested 7 days after implantation of V Low, V Med, and V High clones.

Increased astrocyte proliferation is paralleled with reduced infiltration of immune cells in mice with inducible SMOM2 expression in astrocytes at 5 dpi.

Reactive oxygen species inhibit miR‐199a and miR‐125b expression levels in vitro and in vivo. Reactive oxygen species inhibit miR‐199a and miR‐125b expression.

AβOs induce hypothalamic inflammation, eIF2α phosphorylation and impaired insulin signaling A, BWestern blot analysis of eIF2α‐P levels in the hypothalamus.

Effects of GM1 on astroglial and microglial markers

Stat3 promotes lung fibrosis and collagen synthesis independent of Smad3.A.Masson's trichrome stained sections of lung from gp130wt (wt), Smad3−/− (Smad3−/−),

TFE3 prevents diet‐induced obesity and metabolic syndrome

The BET inhibitor JQ‐1 in combination with the MEK inhibitor PD synergistically impairs cell proliferation and induces apoptosis of NRAS‐mutant.

IFN‐β ameliorates microgliosis and inhibits choroidal neovascularization IFN‐β ameliorates microgliosis and inhibits choroidal neovascularization AExperimental.

Mitochondrial ceramide is involved in HPV‐E7‐mediated lethal mitophagy

Combination of OTX‐015 and PD does not induce overt toxicity

Stat3C/C mice are more prone to Th17 differentiation. A

Tgf‐β inhibition through losartan effectively relieves inflammation in RDEB AQuantification of Cd11b‐positive cells shows that losartan treatment significantly.

Treatment with FGF21‐encoding vectors reverses hepatic steatosis and fibrosis Treatment with FGF21‐encoding vectors reverses hepatic steatosis and fibrosis.

BETi potentiate the efficacy of MEKi in NRAS‐mutant melanoma

Nec‐1 inhibits the phosphorylation and aggregation of tau

Fig. 5 Local gel scaffold for T cell memory response.

The psoriatic phenotype of DKO

Isabelle Plaisance et al. BTS 2016;j.jacbts

Effects of in vivo Nutlin‐3 treatment on CSC content of Numb− and Numb+ tumors Effects of in vivo Nutlin‐3 treatment on CSC content of Numb− and Numb+

DeathPro dyes Hoechst and PI do not affect organoid growth and drug responses DeathPro dyes Hoechst and PI do not affect organoid growth and drug responses.

EGFL7 promoted density and maturation state of glioma vessels

Normal priming and expansion of functional T cells in the spleen during concurrent co‐infection Normal priming and expansion of functional T cells in the.

IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice IL‐23‐induced psoriasis‐like skin disease is ameliorated in IL‐23−/− mice ARepresentative.

Improvement of shHBV7 specificity and efficiency by co‐expression of a TuD against the sense strand Improvement of shHBV7 specificity and efficiency by.

Hccs downregulation induces apoptosome‐independent caspase‐9 activation in mitochondria.A–C.TUNEL assays on st30 embryos co‐injected with hccs‐MO and Apaf1‐MO.

High‐fat diet augments gut permeability, metabolic endotoxemia, and systemic inflammation High‐fat diet augments gut permeability, metabolic endotoxemia,

HPV‐E7 targets RB for induction of ceramide‐dependent mitophagy

Modular Three-component Delivery System Facilitates HLA Class I Antigen Presentation and CD8+ T-cell Activation Against Tumors Benjamin J Umlauf, Chin-Ying.

R1441G‐LRRK2 co‐localizes with Rab10 in the periphery and Rab8 near the nucleus R1441G‐LRRK2 co‐localizes with Rab10 in the periphery and Rab8 near the.

Oligo#5 enters neurons after ICV injection, changes 5HT2C splicing and food intake Oligo#5 enters neurons after ICV injection, changes 5HT2C splicing and.

TUG‐891 increases oxygen consumption by brown adipocytes, mediated by direct UCP1 activation and mitochondrial fragmentation TUG‐891 increases oxygen consumption.

I.c.v.‐injected AβOs induce increased food intake, hypothalamic expression of orexigenic neuropeptides but no hypothalamic cell degeneration AAccumulated.

Both the number and the OX40L expression level of bronchiolar progenitors were increased by influenza infection. Wild‐type mice were intratracheally infected.

Microglial P2X4R modulates oligodendrocyte differentiation

P2X4R blockade increases autoimmune inflammation

Growth of TKI‐resistant EGFR delE746‐A750 PC‐9 NSCLC cells is associated with elevated cellular free fatty acid and expression of FASN, and is suppressed.

LXRα enhances glucose uptake and O‐GlcNAc signaling via activation of the hexosamine biosynthetic pathway (HBP) in cultured cardiomyocytes LXRα enhances.

T22‐GFP‐H6‐FdU‐induced depletion of CXCR4‐overexpressing cancer cells in tumor tissue T22‐GFP‐H6‐FdU‐induced depletion of CXCR4‐overexpressing cancer cells.

The MEK inhibitor CI‐1040 reverses thickening, fibrosis, and angiogenesis in the parietal peritoneum of Cav1−/− mice upon exposure to PD fluidWT or Cav1−/−

Favorable safety profile of LV‐mediated intracerebral gene therapy

Impact of ONX 0914‐induced neutrophilia during CVB3 infection

DIA1(R1204X) induces elongated and thick microvilli in HeLa cells

Effect of D‐Asp treatment on myelination and remyelination in cerebellar organotypic slices Effect of D‐Asp treatment on myelination and remyelination.

Mpdz was localized proximal to the apical surface of CPECs

CAR T‐cell generation in human PBMC‐transplanted mice

Absence of miR‐21 in macrophages promotes foam cell formation

SEMA3C regulates prostate cancer cell growth

Inflammation is associated with increased basal-cell plasticity in the Nkx3.1 mutant prostate. Inflammation is associated with increased basal-cell plasticity.

Niacin suppresses pro‐inflammatory cytokine expression in macrophages in DSS‐induced colitis in mice Niacin suppresses pro‐inflammatory cytokine expression.

Presentation transcript:

Systemic inflammation activates the exosomal machinery in the choroid plexus Systemic inflammation activates the exosomal machinery in the choroid plexus ARepresentative confocal images of CD63, RAB5, and ANXA2 (red) in the choroid plexus (CP) at 0, 4, and 8 h after LPS treatment. Hoechst (blue) was used to stain the nucleus. The dotted line indicates the ependymal cells that line the ventricle and the square boxes indicate the zoomed insert images displayed at the right corner of each image. Scale bars, 100 μm.B, CRepresentative TEM images showing the presence of MVBs in the CPE cells before (B) and 6 h after (C) LPS administration in vivo. Black arrow heads point to exosomes present in MVBs. Scale bars, 9 μm.D–FQuantification of number of MVBs per cell section (D), number of exosomes per MVB (E), and number of exosomes per cell section (F), based on TEM analysis of several adjacent cells (0 h, n = 20; 3 h, n = 21; 4 h, n = 13; 6 h, n = 23).G–JQuantitative real‐time polymerase chain reaction (qPCR) analysis of miR‐1a (G), miR‐9 (H), miR‐146a (I), and miR‐155 (J). Data are presented as relative expression normalized with housekeeping miRs by TaqMan qPCR assay (0 h, n = 4; 1 h, n = 5; 6 h, n = 5; 24 h, n = 3).KNanoSight analysis of CSF isolated from LPS‐injected mice followed by icv injection of vehicle or GW4869, a neutral sphingomyelinase inhibitor that inhibits exosome secretion (n = 8).LqPCR analysis of the expression of miR‐1a, miR‐9, miR‐146a, and miR‐155 in the choroid plexus of mice injected with LPS and then icv injected with vehicle (black) or GW4869 (gray) (n = 4).MNanoSight analysis of the supernatant of choroid plexus explants from PBS‐ or LPS‐injected mice (n = 6).Data information: Data in (D–M) are displayed as mean ± SEM and analyzed by Student's t‐test. Significance levels are indicated on the graphs: *0.01 ≤ P < 0.05; **0.001 ≤ P < 0.01; ***0.0001 ≤ P < 0.001; ****P < 0.0001. Sriram Balusu et al. EMBO Mol Med. 2016;8:1162-1183 © as stated in the article, figure or figure legend