DNA damage foci in irradiated cells.

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Farmer et al Supplementary Figure 1 KU PARP-1 IC 50 = 3.2nM KU PARP-1 IC 50 = 3.4nM KU PARP-1 IC 50 = 730nM a b

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DNA damage foci in irradiated cells. DNA damage foci in irradiated cells. A, detection of ionizing radiation–induced RAD51, 53BP1, and γ-H2AX foci in BRCA1-proficient KP3.33 cells (5), a cell line derived from a KB1PM5 control tumor (control 1) and a 53BP1-negative cell line derived from the olaparib-resistant KB1PM5 tumor (ol-res 1). For characterization of the cell lines, see also Supplementary Fig. S5. Images show the maximum projection, covering the whole cell in the z-direction. B, quantification of RAD51 focus formation of 3 KB1PM5 control cell lines and 3 KB1PM5 olaparib-resistant cell lines in 2 independent experiments. The 3 control cell lines are combined in one bar, and each olaparib-resistant cell line is shown separately for both experiments. C, quantification of RAD51 IRIFs in a control KP tumor and in matched olaparib-sensitive and -resistant KB1PM tumors. See also Supplementary Fig. S6. IR, ionizing radiation. Janneke E. Jaspers et al. Cancer Discovery 2013;3:68-81 ©2013 by American Association for Cancer Research