DNA ANALYSIS LAB TECHNIQUES

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Presentation transcript:

DNA ANALYSIS LAB TECHNIQUES By Kinzie Martin

What are restriction enzymes and how do they cut DNA? Restriction enzymes come from bacteria and are cut DNA in different places.

What is PCR and how does it amplify DNA? PCR (short for Polymerase Chain Reaction What polymerase means>/ break it down by looking up the roots poly, mer, and ase? Poly- means many or much Mer -means part Ase- ending on enzyme names

PCR crime scene RFLPs

What are RFLPs and how are they used to compare DNA? Restriction Fragments Length Poly morph ism (many difference conditions) the length from each person are different and that’s what tells a DNA fingerprint apart the DNA is cut by restriction enzymes and the fragments are RFLPs. The tell us the difference between two people

What is gel electrophoresis and how is used/interpreted? Gel electrophoresis make RFLPs visible by separating them It separating by the size. You can separate the DNA fragments by applying an electric current to the gel. DNA will migrate to the positive side.

Gel Electrophoresis Gel electrophoresis separates RFLPs by electric current Electrophoresis (Electro- electricity phore-carry sis-condition) the condition of carrying DNA using electricity. By a pulling of a positive charge The phosphate in DNA give it an negative so it can be pulled to the positive end. The smallest pieces move the farthest because they are easier to pull.