Lab Automation Software SoftLinx V Flow-Chart Driven Lab Automation Software Change date and company as appropriate. This is an overview presentation about Hudson Control Group. It covers the company and the product and services. Alan H. Katz 10/13/10 November 11, 2018 ©2010 Hudson Robotics, Inc.

SoftLinx V Introduction Set-Up and Initialize Instruments - Add Instruments - Define communications - Setup machine-specific parameters Design Protocol(s) - Drag Instrument methods into the workflow - Setup method-specific parameters - Create/Modify variables to add complexity to workflows - Create/Configure control structures (loops, conditionals) - Create VBA code to further customize workflows Run Protocol(s) - Keep Track of every Microplate in the system - Maintain Database of all protocols and method steps - Run in simulate mode to test off-line - Prevent standstills or collisions November 11, 2018 ©2010 Hudson Robotics, Inc.

1. Setup & Initialize Instruments – Add Instruments Hundreds of available instrument interfaces - or make your own (VBA) November 11, 2018 ©2010 Hudson Robotics, Inc.

1. Setup & Initialize Instruments – Define Communications November 11, 2018 ©2010 Hudson Robotics, Inc.

1. Setup Instruments – Machine-Specific Parameters (PlateCrane) November 11, 2018 ©2010 Hudson Robotics, Inc.

1. Setup Instruments – Machine-Specific Parameters (Shaker Nest) November 11, 2018 ©2010 Hudson Robotics, Inc.

2. Design Protocols - Drag and Drop Method Steps into WorkFlow Eg. Add Micro10x Dispense Step November 11, 2018 ©2010 Hudson Robotics, Inc.

2. Design Protocols – Setup Method-Specific Parameters November 11, 2018 ©2010 Hudson Robotics, Inc.

2. Design Protocols – Create Variables for More Control Supports: Numbers, Strings and Booleans November 11, 2018 ©2010 Hudson Robotics, Inc.

2. Design Protocols – Modify Variables for More Control Variables can come from various places: Loop counters Data Files Instrument Interface Variables November 11, 2018 ©2010 Hudson Robotics, Inc.

2. Design Protocols – Create and Configure Loops Loops can be given descriptive names Loops can be run: Forever A Predetermined # of times A Variable # of times November 11, 2018 ©2010 Hudson Robotics, Inc.

2. Design Protocols – Create Conditional Statements Conditionals can be given descriptive names November 11, 2018 ©2010 Hudson Robotics, Inc.

2. Design Protocols – Parallel Processes Processes can be given descriptive names Parallel Processes can be run: At the same time In a Predetermined order In order defined by a variable November 11, 2018 ©2010 Hudson Robotics, Inc.

2. Design Protocols – Add Your Own VBA Scripts Send & Return parameters to and from VBA Interface November 11, 2018 ©2010 Hudson Robotics, Inc.

2. Design Protocols – eg ChemoRobotics VBA Script Predicted Actives Molecular Modeling Cherry-Pick Update Models Screen Hits Confirmed Actives November 11, 2018 ©2010 Hudson Robotics, Inc.

2. Design Protocols – ChemoRobotics User Interface (VBA) Panel to control the iterative process Calculates and makes all docking grids available Biological data retrieved from reader by SoftLinx List of compound source locations for each hit Sets up and runs Glide. Then Generates list of all unique hits with desired score Sets up and runs Shape Search queries for each Docking hit Running total of unique hits to be screened Performs second round of Shape Search queries based on hits from first round November 11, 2018 ©2010 Hudson Robotics, Inc.

ChemoRobotics Architecture - SoftLinx & VBA User Interface Cherry-Picking & Screening Molecular Modeling via Knime November 11, 2018 ©2010 Hudson Robotics, Inc.

2. Design Protocols – Other Features One-Click Deactivate a Step Method Skipped Active Method November 11, 2018 ©2010 Hudson Robotics, Inc.

2. Design Protocols – Other Features Send an E-Mail Message Provide updates of protocol status Alert user of operation issues Archive protocol information at various intervals November 11, 2018 ©2010 Hudson Robotics, Inc.

2. Design Protocols – Other Features Other Types of Method Steps Insert a delay Run and external program or batch file Stop the protocol November 11, 2018 ©2010 Hudson Robotics, Inc.

2. Design Protocols – Other Features Complete Regions can be De-Activated with One Click Group Methods into Regions Regions can have descriptive names November 11, 2018 ©2010 Hudson Robotics, Inc.

3. Run Protocols - Keep Track of Every Plate & Protocol Plates Database stores plate location after each move Reports can be generated to trace the history of any plate Protocols & Methods Potential to recover from a power outage Structure of protocol recoverable - even if file was not saved! Consistent with 21 CFR Part 11 November 11, 2018 ©2010 Hudson Robotics, Inc.

3. Run Protocols - Avoid Standstills and Collisions Eg. 2 PlateCranes Remove a plate from the SOLO SoftLinx Reserves the SOLO Nest position before a Method is run Second PlateCrane must wait until the nest is released Standstills Eg. 2 PlateCranes are waiting for a plate to be removed from the SOLO SoftLinx System Move Tool finds available nest to temporarily move plate November 11, 2018 ©2010 Hudson Robotics, Inc.