Piotr Knapp Piotr Wróblewski

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Presentation transcript:

Piotr Knapp Piotr Wróblewski Relationship between radiation dose and chromosome aberration frequency – Calibration curves Piotr Knapp Piotr Wróblewski Supervisors: Ekaterina Zaytseva, Polina Kutsalo, Marta Deperas-Kamińska Laboratory of Radiation Biology

The fate of irradiated cell Necrotic death After high dose Somatic effect Healthy cell No Micronuclei Mitotic death No effect Cancer Gy Correct repair Incorrect repair Cell with damaged DNA Stable chromosomal Aberrations - Mutations Unstable chromosomal aberrations Apoptotic death Mutation path Somatic effect In the germ cells Somatic effect Carcinogenic effect Genetic effect

Aim of the project Creation of dose dependency calibration curve of chromosomal aberrations in human peripherial blood lymphocytes exposed to protons beam (LET≤100keV/µm) performed at Phasotrone (LNP, JINR) Biological dosimetry - Allow to assess an accidental radiation dose on base of the level of changes in chromosome structure.

Scheme of the experiment Culture of irradiated blood BrdU Colcemid Fixation 0-24h 48h 48-50h Staining with Fluorescence Plus Giemsa Watching

Karyogram

Chromosomal aberrations

An example of cell stained with Giemsa dye with dicentric chromosome.

Our Calibration Curve Aberration per cell DOSE [Gy]

Special thanks to: Marta Deperas-Kamińska Polina Kutsalo Ekaterina Zaytseva

Thank you for your attention!