MiR-155-5p positively regulates colon cancer cell migration by targeting RhoA 2016 Molecular Biomarkers, Berlin | AMR AL-HAIDARI | Department of Clinical.

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MiR-155-5p positively regulates colon cancer cell migration by targeting RhoA 2016 Molecular Biomarkers, Berlin | AMR AL-HAIDARI | Department of Clinical Sciences, Surgery research unit Department of Clinical Sciences, Section of Surgery 16th Sep Amr Al-Haidari

Facts and figures 1 Up to date 2500 miRNAs Identified and only 600 characterized 3 Role in cancer Proliferation, cell cycle regulation, migration, survival 2 Function Regulate gene expression 4 Colon cancer Third leading cause of death 90% metastasis Department of Clinical Sciences, Section of Surgery 16th Sep Amr Al-Haidari

Key players of the paper Chemokine CCL17 RhoA AU-rich elements 3′-UTRs of many but not all mRNAs

Mechanisms of miRNA positive regulation Seahyoung Lee, Eunhyun Choi, et al. (2015), Implications of MicroRNAs in the Vascular Homeostasis and Remodeling, Muscle Cell and Tissue Department of Clinical Sciences, Section of Surgery 16th Sep Amr Al-Haidari

RhoA regulation 78% 77% 80% cellular conditions depends on Serum condition 2-folds 78% Serum free condition 77% 80%

Non toxic transfection miR-155-5p regulates colon cancer cell migration Non toxic transfection Department of Clinical Sciences, Section of Surgery 16th Sep Amr Al-Haidari

RhoA is a direct target of miR-155-5p Department of Clinical Sciences, Section of Surgery 16th Sep Amr Al-Haidari

AREs are involved in RhoA mRNA regulation Department of Clinical Sciences, Section of Surgery 16th Sep Amr Al-Haidari

RhoA mRNA and miR-155-5p 80% are associated with Ago2 4.5-folds Department of Clinical Sciences, Section of Surgery 16th Sep Amr Al-Haidari

Mechanisms of miRNA positive regulation Serum condition Serum condition Ago2 Dicer TRBP GW182 S AAAA Hur RhoA mRNA TTP TNRC6A miR-155-5p TNRC6A Ago2 Dicer TRBP GW182 S AAAA Hur miR-155-5p RhoA mRNA TTP Serum free condition FXR1 Department of Clinical Sciences, Section of Surgery 16th Sep Amr Al-Haidari

Conclusion miR-155-5p positively regulates CCL17-induced RhoA activity and migration of serum starved colon cancer cells Effect of miR-155-5p was found to be mediated by specific ARE elements present in the 3´-UTR region of RhoA mRNA. Targeting miR-155-5p could open new strategies in antagonizing colon cancer metastasis Department of Clinical Sciences, Section of Surgery 16th Sep Amr Al-Haidari

Thanks Department of Clinical Sciences, Section of Surgery 16th Sep Amr Al-Haidari