RE Digestion Field Trip Results Lodi High School Mr. Olson’s Group March 8, 2011
Lodi HS Student Data: Team Kate 1 2 3 4 5 6 Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 10l Negative (No Enzyme) Control Sample ? E / H Lambda Digest Marker Team Kate: Observations: This gel is almost completely blank, indicating that DNA was likely omitted from the digests. Additionally, any DNA fragments in the Lane 5 digest marker are scarcely visible, indicating that gel loading was also a problem for this group.
Lodi HS Student Data: Tiggers 1 2 3 4 5 6 Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 10l Negative (No Enzyme) Control Sample ? E / H Lambda Digest Marker Team Tiggers Observations: While some DNA fragments are visible in Lane 3, they cannot be interpreted due to the absence of the Lane 1 or Lane 5 control samples that would give a frame of reference to those bands. This group likely had issues with pipette technique, both in setting up the reactions and in gel loading.
Lodi HS Student Data: Team A 1 2 3 4 5 6 Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 10l Negative (No Enzyme) Control Sample ? E / H Lambda Digest Marker Team A Observations: Lane 5, while faint, shows the approximate banding pattern of DNA fragments that we would expect to see in the digest marker. Lane 1 shows a single band, which we expect to see in the negative control sample. Lane 4 shows a banding pattern characteristic of PstI, since the largest fragment in Lane 6 is smaller than the largest fragment in the Lane 5 digest marker. Lane 3 shows, faintly, the compact banding pattern that is characteristic of the EcoRI digest. Lanes 2 & 6 show no DNA fragments and cannot be interpreted. It is likely that DNA was not added to these digests.
Lodi HS Student Data: Dr Scholls 1 2 3 4 5 6 Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 10l Negative (No Enzyme) Control Sample ? E / H Lambda Digest Marker Team Dr. Scholls Observations: Lane 5 shows the banding pattern expected from the digest marker. Lane 1 shows, faintly, one large uncut band, which is what we expect from the negative control Lane 3 looks very similar to the negative control in Lane 1, indicating that it did not receive restriction enzyme. Lane 2 shows the compact banding pattern of DNA fragments that is associated with the EcoRI digest of Lambda DNA. While faint, Lane 4 shows the banding pattern expected by HindIII digest. The largest HindIII digest fragment being larger than the largest fragment visible in the Lane 5 digest marker. Lane 6 is lacking any visible DNA fragments, indicating that this digest likely did not receive sufficient DNA.
Lodi HS Student Data: CCAN 1 2 3 4 5 6 Lane 1 Lane 2 Lane 3 Lane 4 Lane 5 Lane 6 10l Negative (No Enzyme) Control Sample ? E / H Lambda Digest Marker Team CCAN: Observations: Lane 1 has one bright solitary band, which is what we expect to see in the negative control well Lane 2 also has one bright solitary band, indicating that while DNA was added to the reaction tube, restriction enzyme was not. Lane 5 has a set of DNA fragments. Though faint, they show a pattern that approximates the Lambda digest marker. Lane 4 has a bright set of bands that clearly show the banding pattern of fragments seen in the digest marker. This is a clear indicator that lane 3 is the HindIII/EcoRI double digest. Lane 6 appears to have the banding pattern of DNA fragments that is characteristic of HindIII digest of Lambda DNA, since the largest fragment is larger than the largest fragment visible in the Lane 5 digest marker. Lane 3 barely has any visible DNA fragments. Those present (if present) are to faint to characterize.