3 mm holes ________________ distance water traveled Bellwork:Tues. Aug. 23, 2016 Mario studied how far room temperature water would spurt out of a plastic milk carton when 3mm holes were punched at different heights from the bottom of the container. Identify the independent / dependent variables/control. write the red and fill in with the green: 3 mm holes ________________ distance water traveled out of carton______________________ different heights of holes in container___________ https://www.youtube.com/watch?v=2tBWfqKA0Tk

https://www.youtube.com/watch?v=qtLnBz6lbRQ Name the independent variable and dependent variable the two experiments. 1. I.V: D.V.: 2. I.V: D.V.:

Question: Responding variable Manipulated variable

Using the Light Microscope

Oil Immersion objective Do not use!!!!

X the eyepiece

Now, about the magnification… Low power Medium power High power Oil immersion objective which you DO NOT USE

Notes: draw this little table… Objective Magnification Eye Piece Total Magnification Low Power X4 X10 = Medium Power X10 High Power X40 X10 = DO NOT USE OIL IMMERSION!!!! (The longest lens) IT WILL RUIN THE SCOPE AND YOU WILL HAVE PAY $$$$$$$$! 40X 100X 400X

How to Focus Your Microscope ALWAYS start with lowest power (red) objective lens While looking from the side, move the lens down as close to the specimen as possible without touching it.  Look through eyepiece lens and focus upward only until the image is sharp.  If you can't get it in focus, repeat the process again.    

Move to the next highest objective Focus medium power objective (yellow) with fine adjustment only. If you need more adjustment, once again move the lens down as close to the specimen as possible without touching it, and look through the eyepiece lens and focus upward only until the image is sharp.  If you can't get it in focus, repeat the process again.    

Now to Change to the High Power Objective… turn to the “blue” objective, only use the fine focus (small knob with this objective. You must be very careful not to crack the slide

AND DONT USE THE Longest Objective (grey or white) Oil immersion objective it will break the slide and the lens and you will have to pay for damages

Adjusting the Diaphragm : adjust to allow the amount of light to enable you to see the specimen the best

e e Lower a clean cover slip over the drop at a 45 degree angle as though it were hinged on one side.

Air Bubble this is what an edge of bubble looks like

Explain How to Make a Wet Mount Slide: Put tiny amount of specimen on slide Add drop of water to slide Drop cover slip on from side at 45 degree angle

https://www.youtube.com/watch?v=QrmashOX5EU

Bellwork: Wed. Aug. 24, 2016 Low power objective AKA the ________________ objective & its stripe is _________. Medium power has magnification of ___________ & a __________ stripe. High power has magnification of ___________ & a __________ stripe. The objective you don’t use is the _____________ & it has a ____________ stripe.

https://www.youtube.com/watch?v=9va0KPrVExs https://www.youtube.com/watch?v=8-oCZ_JEgbM

Scanning electron microscope https://www.youtube.com/watch?v=GY9lfO-tVfE

Transmission electron microscope https://www.youtube.com/watch?v=j2A6KeWrqeM

40X 100X 400X

In your first non-living circle: This is a picture of a letter e shown at 40X. This slide was set up with the letter in the correct orientation. Notice that it appears upside down when viewed under the microscope.

40X 100X 400X

This is a picture of the letter "e" shown at 100X This is a picture of the letter "e" shown at 100X. Notice, that as you increase the power of the lens, your field of view gets smaller

40X 100X 400X

Does your diaphragm have numbers? NO 1. 2. 3. scanning 40X red 100X low yellow high 400X blue Does your diaphragm have numbers? NO 32

In one of your “Living” circles make and draw a yeast slide

First, make your yeast slide… Put a very small drop of yeast/sugar/water on your slide

Lower a clean cover slip over the drop at a 45 degree angle as though it were hinged on one side.

The finished slides should look like this The finished slides should look like this. Always hold a slide by the sides so you do not get fingerprints all over your specimen you want to view!

For your other living go outside and grab ONE little leaf

Draw what you see under Medium and High Power Name: 100X 400X 100X 400X Then describe what you see, and answer the analysis questions

Write these questions on the back of your lab paper 1. Did the letter appear in the same orientation when viewed through the microscope as viewed without the microscope? 2. When you move the slide to the right what direction does it appear to move under the microscope? 3. What happened to the image when you switched objectives?

40X 100X 400X Objective Magnification Eye Piece Total Magnification Low Power X4 X10 = Medium Power X10 High Power X40 X10 = DO NOT USE OIL IMMERSION!!!! (The longest lens) IT WILL RUIN THE SCOPE AND YOU WILL HAVE PAY $$$$$$$$! 40X 100X 400X

Bellwork: old For the lab scanning – 40X (red stripe) “low” – 100X (yellow stripe) AND the “diaphragm on our scopes Do NOT have numbers!! What objective do you start with when focusing on a specimen? _____________ What objective do you leave the microscope on before putting the plastic cover back on it? ________________