Microscopes
History of the Microscope 1590 –first compound microscope
History of the Microscope 1655 – Robert Hooke used a compound microscope to observe pores in cork He called them “cells”
History of the Microscope Antoine van Leeuwenhoek 1st to see single-celled organisms in pond water
Microscope Vocabulary Magnification: increase of an object’s apparent size Resolution: power to show details clearly Both are needed to see a clear image
1. Compound Light Microscope 1st type of microscope, most widely used Types of Microscopes 1. Compound Light Microscope 1st type of microscope, most widely used light passes through 2 lenses Can magnify up to 2000x
Ocular lens Objective lenses
Types of Microscopes 2. Electron Microscope Used to observe VERY small objects: viruses, DNA, parts of cells Uses beams of electrons rather than light Much more powerful
Types of Microscopes Transmission Electron Microscope (TEM) Can magnify up to 1,000,000x Images viewed by photograph or monitor
This transmission electron microscope image reveals mitochondria in a thin section of mammalian lung tissue. Image courtesy of the Dartmouth Electron Microscope Facility Dartmouth College This transmission electron microscope image reveals mitochondria in a thin section of mammalian lung tissue. Image courtesy of the Dartmouth Electron Microscope Facility Dartmouth College
Scanning Electron Microscope (SEM) Types of Microscopes Scanning Electron Microscope (SEM) Can magnify up to 200,000x Produces realistic 3-D images Images viewed by photograph or monitor
A scanning electron microscope image of a monarch butterfly wing. Since a scanning electron microscope only collects a black and white image (representing intensity of electrons) the image must be colorized with photoshop.