Microscopes Tools of Science

History of Microscope The compound light microscope was invested by Hans and Zacharias Janssen 1590-1610 The first microscopes were novelty toys sold to the wealthy The image quality was poor

Robert Hooke – 1660’s Chemist, mathematician, physicist, and inventor Improved the CLM Coined the term cells We will talk more about him

Anton van Leeuwenhoek – 1670’s Wine surveyor, cloth merchant, minor public officer Used a single lens microscope Made the best scopes available

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Parts of the Microscope Explained Ocular Lens The lens you look into Usually a power of 10x Revolving nosepiece Contains different objectives Stage Where slides are placed Stage clips Holds slides in place Objective lens One of 3 or 4 lenses on a microscope They have different powers Diaphragm Adjusts amount of light illuminating specimen

To find Total Magnification Multiple ocular x objective If the ocular lens is 20x and the objective lens is 100x, what is the total magnification? If total magnification is 300x and the ocular lens is 100x, what is the ocular lens?

Stereomicroscope Contains ________ ocular lenses Total magnification is under 100x Gives a 3-D image

Electron Microscopes Scanning Electron Microscope Up to 300,000x 3-D image of specimen _____________________ _____________________ Transmission Electron Microscope Highest possible magnification 2-D image ____________________ ____________________

Scanning electron microscope Sunflower pollen Dust mite

Important Note: Your microscope lab is due Tuesday September 11, 2012! Plan to stay after school on Monday September 10th to finish if needed… NO excuses!