Membrane Transport Dr. Kevin Ahern.

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Presentation transcript:

Membrane Transport Dr. Kevin Ahern

Chemical and Electrical Potential Across a Lipid Bilayer Barrier

Osmotic Pressure Across a Semipermeable Barrier

Types of Membrane Proteins

Three Types of Membrane Protein Ports Symport (Synport) Uniport Antiport

No Net Change in Charge Net Change in Charge

Ion Channels

Ion Channel Features 1. General Structure 2. Opening 3. Filter 4. Effective Opening Size 5. Door

Closed Open Potassium Channel

Potassium Channel - Top-down View

Hydration Shell

Cellular Transport Types Passive - Purely Diffusion Driven, Follows Concentration Gradient Facilitated - Protein Guided, Follows Concentration Gradient Active - Protein Guided, Energy Required to Move at Least One Molecule Against Concentration Gradient

Passive Transport - Purely Diffusion Driven

Ion Channels vs Transporters

Facilitated Diffusion

Active Transport

Sodium-Potassium ATPase Higher K+ inside Higher Na+ outside Sodium-Potassium ATPase

Sodium/Glucose Symport Secondary Energy Source Sodium-Glucose Pump Sodium/Glucose Symport

Lactose/Proton Symport Lactose Permease Lactose/Proton Symport

Secondary Energy Source - Protons Lactose Permease Secondary Energy Source - Protons

Voltage Change

Nerve Cell Transmission 1. Stimulus 2. Opening of Na+ gates 3. Na+ diffuses into cell 4. Voltage change causes K+ gates to open 5. K+ diffuses out of cell 6. All gates close 7. Wave of voltage changes moves down cell 8. Neurotransmitters move signal across cells 9. Na+ K+ ATPase restores gradients in originating cell

Stimulus, Na+ gates open K+ gates open Action Potential Stimulus, Na+ gates open Na+K+ ATPase acts

Transmission of signal from one cell to another Gates Gates Wave of Na+ in, K+ out Transmission of signal from one cell to another

Bind Voltage Gated Na+ Channels Tetrodotoxin From Puffer Fish Bind Voltage Gated Na+ Channels

Shellfish Toxin From Algal Blooms Sodium Channel Blocker Saxitoxin Shellfish Toxin From Algal Blooms Sodium Channel Blocker