Discuss formal report assignment Get acquainted with the library

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Presentation transcript:

Discuss formal report assignment Get acquainted with the library Lab 9 Goals and Objectives: Discuss formal report assignment Get acquainted with the library Make new streak for isolation plate of each unknown Finish getting Gram reaction results for each unknown Discuss observations of the 12 possible organisms Discuss how to rule some out Start working on a dichotomous key of logic for solving all 12 unknowns

1 2 3 4 5 6 7 8 9 10 11 12 Bacillus subtilis Enterobacter cloacae Escherichia coli Klebsiella pneumoniae 5 6 7 8 Proteus vulgaris Pseudomonas aeruginosa Salmonella typhimurium Shigella flexnari 9 10 11 12 Staphylococcus aureus Staphylococcus epidermidis Enterococcus faecalis Streptococcus pyogenes

The Dichotomous Key -a visible map of logic flow -divides a group of possible things into smaller and smaller groupings until it leads to the identification of just one -tracing the pathway backwards shows all the characteristics that collectively are unique to only that one -assesses only one characteristic per branch -lines all the answers up across the bottom of the key

External covering: feathers External covering: hair Example: explain with the least number of characteristics how to tell the difference between an old lady and her three pets: sort a human, a cat, a dog and a bird. Construct a Dichotomous key to ascertain the identity of each from this small group: Animals in the house External covering: feathers External covering: hair No tail Tail Meow Woof Bird Human Cat Dog

12 Possible Unknowns

1 2 3 4 5 6 7 8 9 10 11 12 Bacillus subtilis Enterobacter cloacae Escherichia coli Klebsiella pneumoniae 5 6 7 8 Proteus vulgaris Pseudomonas aeruginosa Salmonella typhimurium Shigella flexnari 9 10 11 12 Staphylococcus aureus Staphylococcus epidermidis Enterococcus faecalis Streptococcus pyogenes

12 Possible Unknowns Gram Positive Gram Negative

12 Possible Unknowns Gram Positive Gram Negative

12 Possible Unknowns Gram Positive Gram Negative Gelatinase + Pseudomonas aeruginosa Bacillus subtilis

1 2 3 4 5 6 7 8 9 10 11 12 Bacillus subtilis Enterobacter cloacae Escherichia coli Klebsiella pneumoniae 5 6 7 8 Proteus vulgaris Pseudomonas aeruginosa Salmonella typhimurium Shigella flexnari 9 10 11 12 Staphylococcus aureus Staphylococcus epidermidis Enterococcus faecalis Streptococcus pyogenes