3. PCR Page 376 – 377.

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3. PCR Page 376 – 377

PCR Polymerase Chain Reaction PCR – in vitro The more DNA available, the easier it is to work with.

Kary Mullis Developed the PCR process in 1986 Nobel Laureate, 1993

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Three steps for a cycle DNA strand denaturation (95°C) Primer annealing (50°C - 65°C) DNA strand synthesis (72°C)

PCR

Problem Where do you find enzymes that don’t break down at 95°C? Thermus aquaticus Isolated Taq polymerase from these bacteria.

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