Validation of a four-primer real-time PCR as a diagnostic tool for single and mixed Plasmodium infections L. Cnops, J. Jacobs, M. Van Esbroeck Clinical.

Slides:

Advertisements

Similar presentations

Models for the organisation of hospital infection control and prevention programmes B. Gordts Clinical Microbiology and Infection Volume 11, Pages

Advertisements

C.-S. Lee, J.-H. Lee Clinical Microbiology and Infection

P.-Y. Lévy Clinical Microbiology and Infection

Twelve years of dengue surveillance in Belgian travellers and significant increases in the number of cases in 2010 and 2013 J. Verschueren, L. Cnops,

R. Dumke, H. von Baum, P.C. Lück, E. Jacobs

A. Bergman, D. Heimer, N. Kondori, H. Enroth

Malaria rapid diagnostic tests in travel medicine

Validation of PCR–reverse line blot, a method for rapid detection and identification of nine dermatophyte species in nail, skin and hair samples A.M.C.

Gut bacterial microbiota and obesity

M. Cogliati, R. D'Amicis, A.M. Tortorano

Migrant health—a cause for concern?

M.L. McMorrow, M. Aidoo, S.P. Kachur

A.G. Koek, S.M. Bruisten, M. Dierdorp, A.P. van Dam, K. Templeton

C.-S. Lee, J.-H. Lee Clinical Microbiology and Infection

A.K. Reddy, P. Garg, I. Kaur Clinical Microbiology and Infection

J.-P. Van geertruyden Clinical Microbiology and Infection

O. Barraud, M. Casellas, C. Dagot, M.-C. Ploy

Virological tools to diagnose and monitor hepatitis C virus infection

B.J. Kocjan, D. Bzhalava, O. Forslund, J. Dillner, M. Poljak

O. Clerc, G. Greub Clinical Microbiology and Infection

Association between particular polymorphic residues on apical membrane antigen 1 (AMA-1) and platelet levels in patients with vivax malaria P. Grynberg,

Validation of PCR–reverse line blot, a method for rapid detection and identification of nine dermatophyte species in nail, skin and hair samples A.M.C.

A PCR-based method to differentiate between Acinetobacter baumannii and Acinetobacter genomic species 13TU P.G. Higgins, H. Wisplinghoff, O. Krut, H.

B.J. Kocjan, D. Bzhalava, O. Forslund, J. Dillner, M. Poljak

P. Balraj, G. Vestris, D. Raoult, P. Renesto

Louis Pasteur, from crystals of life to vaccination

How to evaluate and predict the ecologic impact of antibiotics: the pharmaceutical industry view from research and development R. Bax Clinical Microbiology.

Should standardized susceptibility testing for microbial biofilms be introduced in clinical practice? T. Coenye, D. Goeres, F. Van Bambeke, T. Bjarnsholt

Improved performance with saliva and urine as alternative DNA sources for malaria diagnosis by mitochondrial DNA-based PCR assays C. Putaporntip, P.

Vector control: a cornerstone in the malaria elimination campaign

Molecular mechanism of gentamicin resistance in Bartonella henselae

Training for the infectious diseases speciality in Norway

A new multiplex PCR for easy screening of methicillin-resistant Staphylococcus aureus SCCmec types I–V K. Boye, M.D. Bartels, I.S. Andersen, J.A. Møller,

Prevalence of the sat, set and sen genes among diverse serotypes of Shigella flexneri strains isolated from patients with acute diarrhoea S.K. Niyogi,

B. Gordts Clinical Microbiology and Infection

Characterization of two novel gene cassettes, dfrA27 and aadA16, in a non-O1, non- O139 Vibrio cholerae isolate from China J. Sun, M. Zhou, Q. Wu, Y.

Validation of an apicoplast genome target for the detection of Plasmodium species using polymerase chain reaction and loop mediated isothermal amplification

E. López-Camacho, Z. Rentero, G. Ruiz-Carrascoso, J. -J. Wesselink, M

Development and clinical validation of a molecular diagnostic assay to detect CTX-M- type β-lactamases in Enterobacteriaceae J.D.D. Pitout, N. Hamilton,

Clinical and microbiological characteristics of Nocardiosis including those caused by emerging Nocardia species in Taiwan, 1998–2008 C.-K. Tan, C.-C.

PCR amplification and high-resolution melting curve analysis as a rapid diagnostic method for genotyping members of the Mycobacterium avium–intracellulare.

Evaluation of a single-tube real-time PCR for detection and identification of 11 dermatophyte species in clinical material A.M.C. Bergmans, M. van der.

New diagnostic tools in schistosomiasis

Metagenomics and probiotics

Effect of antioxidants on the immune response of Helicobacter pylori

Previously unknown species of Aspergillus

Abstracts cont. Clinical Microbiology and Infection

Y. Huang, B. Yang, W. Li Clinical Microbiology and Infection

Real-time PCR assays compared to culture-based approaches for identification of aerobic bacteria in chronic wounds J.H. Melendez, Y.M. Frankel, A.T.

Accurate hepatitis C virus genotyping and selection of optimal therapy: lessons from a St Petersburg strain infection E. Knops, E. Heger Clinical Microbiology.

Twelve years of fluconazole in clinical practice: global trends in species distribution and fluconazole susceptibility of bloodstream isolates of Candida

A.P. Underwood, J. Green Clinical Microbiology and Infection

Clinical infection services—the Leiden experience

C. Wongsrichanalai, C.H. Sibley Clinical Microbiology and Infection

Statin use and clinical outcomes among pneumonia patients

O. Clerc, G. Greub Clinical Microbiology and Infection

K. Kaier, N.T. Mutters, U. Frank Clinical Microbiology and Infection

The atypical pneumonias: clinical diagnosis and importance

Detection of Bartonella henselae and Bartonella quintana by a simple and rapid procedure using broad-range PCR amplification and direct single-strand.

Modelling during an emergency: the 2009 H1N1 influenza pandemic

K. S. Ko, T. Kuwahara, L. Haehwa, Y. -J. Yoon, B. -J. Kim, K. -H

The L2b real-time PCR targeting the pmpH gene of Chlamydia trachomatis used for the diagnosis of lymphogranuloma venereum is not specific to L2b strains

Are we losing the fight against malaria one more time?

J.L. Balcázar Clinical Microbiology and Infection

Three years experience of real-time PCR for the diagnosis of Q fever

Typing of Clostridium difficile

Impact of antibiotic restrictions: the patient's perspective

Comparative study of pediculicidal effect of medical plants

CMI readers' survey Clinical Microbiology and Infection

The future of diagnostic bacteriology

Presentation transcript:

Validation of a four-primer real-time PCR as a diagnostic tool for single and mixed Plasmodium infections L. Cnops, J. Jacobs, M. Van Esbroeck Clinical Microbiology and Infection Volume 17, Issue 7, Pages 1101-1107 (July 2011) DOI: 10.1111/j.1469-0691.2010.03344.x Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions

FIG. 1. Primer and probe sequences. Partial alignment of the Plasmodium falciparum (Pf) (accession number M19172; bp 1067–1209), Plasmodium ovale (Po) (accession number L48987; bp 1079–1223) Plasmodium vivax (Pv) (accession number X13926; bp 1398–1542) and Plasmodium malariae (Pm) (accession number AF488000; bp 1106–1256) 18S rRNA gene around the region of selected primers and probes. The pan-primer PCR uses universal Plasmodium genus forward (FW) (plasmo1) and reverse (plasmo2) primers (bold italic) in combination with four species-specific probes (Falcprobe, Vivprobe, Malaprobe and Ovaprobe) (grey highlights). In the four-primer PCR, four species-specific FW primers (underlined) are used instead of plasmo1, resulting in shorter amplicons (Pf, 100 bp; Pv, 105 bp; Pm, 110 bp; Po, 105 bp). Clinical Microbiology and Infection 2011 17, 1101-1107DOI: (10.1111/j.1469-0691.2010.03344.x) Copyright © 2011 European Society of Clinical Infectious Diseases Terms and Conditions