Differential expression of vascular endothelial growth factor-A isoforms in the mouse uterus during early pregnancy Lisa M. Walter, Peter A.W. Rogers,

Slides:

Advertisements

Similar presentations

Invest. Ophthalmol. Vis. Sci ;44(9): doi: /iovs Figure Legend:

Advertisements

Expression of the novel gene embryo implantation factor 2 (EMO2) in the mouse uterus at the implantation sites Zhaogui Sun, Ph.D., Renwei Su, Ph.D.,

Journal of Surgical Research

S. Lager, V.I. Ramirez, F. Gaccioli, T. Jansson, T.L. Powell Placenta

Prolactin role in the bovine uterus during adenomyosis

Vascular Endothelial Growth Factor-C and Its Receptor VEGFR-3 in the Nasal Mucosa and in Nasopharyngeal Tumors Anne Saaristo, Taina A. Partanen, Johanna.

Recovery from hind limb ischemia is less effective in type 2 than in type 1 diabetic mice: Roles of endothelial nitric oxide synthase and endothelial.

The Cutaneous Vascular System in Chronic Skin Inflammation

Shalmali J. Dharma, M. Sc. , Deepak N. Modi, Ph. D. , Tarala D

Reduced hind limb ischemia-reperfusion injury in Toll-like receptor-4 mutant mice is associated with decreased neutrophil extracellular traps Rahmi Oklu,

Polymorphism of CAG and GGN repeats of androgen receptor gene in women with polycystic ovary syndrome Chun Yuan, Chao Gao, Yi Qian, Ying Liu, Shi-Wen.

Expression and localization of opioid receptors during the maturation of human oocytes Ekaitz Agirregoitia, Laura Peralta, Rosario Mendoza, Antonia Expósito,

Progesterone stimulates expression of follistatin splice variants Fst288 and Fst315 in the mouse uterus R.G. Craythorn, W.R. Winnall, F. Lederman, E.J.

Expression of KRAS in the endometrium of early pregnant mice and its effect during embryo implantation Xia Long, Min Zhang, Xuemei Chen, Junlin He, Yubin.

Differential regulation of Akt phosphorylation in endometriosis

Progesterone regulation of glutathione S-transferase Mu2 expression in mouse uterine luminal epithelium during preimplantation period Hua Ni, Ph.D.,

Effect of vascular endothelial growth factor on sperm motility and survival Ahmet Cem Iyibozkurt, Pelin Balcik, Sibel Bulgurcuoglu, Burcu Kardaş Arslan,

Decreased expression of Wiskott–Aldrich syndrome protein family verprolin-homologous protein 2 may be involved in the development of pre-eclampsia Juan.

Huan Yang, Caixia Lei, Wei Zhang Reproductive BioMedicine Online

VEGF Gene Delivery to Muscle

Stomatin-like protein 2 is involved in endometrial stromal cell proliferation and differentiation during decidualization in mice and humans Qian Feng,

Apolipoprotein A1 and heterogeneous nuclear ribonucleoprotein E1 implicated in the regulation of embryo implantation by inhibiting lipid peroxidation

Urocortin and corticotrophin-releasing hormone receptor type 2 mRNA are highly expressed in deep infiltrating endometriotic lesions Patrizia Carrarelli,

Increased vascularization of shoulder regions of carotid atherosclerotic plaques from patients with diabetes Fredrik J. Olson, PhD, Sofia Strömberg,

Volume 126, Issue 3, Pages (March 2004)

Patrick Kibangou Bondza, Ph. D. , Christine N. Metz, Ph. D

Altered expression of the tachykinins substance P/neurokinin A/hemokinin-1 and their preferred neurokinin 1/neurokinin 2 receptors in uterine leiomyomata

Increase in excitatory amino acid concentration and transporters expression in osteoarthritic knees of anterior cruciate ligament transected rabbits

Effects of HMG on revascularization and follicular survival in heterotopic autotransplants of mouse ovarian tissue Yanrong Wang, Qing Chang, Jing Sun,

Akt expression in mouse oocytes matured in vivo and in vitro

Cellular and molecular mechanism regulating blood flow recovery in acute versus gradual femoral artery occlusion are distinct in the mouse Yagai Yang,

Zhen Tian, Ph. D. , Zhen-Ao Zhao, Ph. D. , Xiao-Huan Liang, Ph. D

Why are reproductive cancers more common in nulliparous women?

Dopamine receptor 2 activation inhibits ovarian vascular endothelial growth factor secretion in an ovarian hyperstimulation syndrome (OHSS) animal model:

Regulation of myeloid ecotropic viral integration site 1 and its expression in normal and abnormal endometrium Linli Hu, M.D., Haixia Li, M.D., CindyTzu-Ling.

Mast cells in human testicular biopsies from patients with mixed atrophy: increased numbers, heterogeneity, and expression of cyclooxygenase 2 and prostaglandin.

Endometrial expression and in vitro modulation of the iron transporter divalent metal transporter-1: implications for endometriosis Carlos Patricio Alvarado-Díaz,

Regulation of inflammatory and angiogenesis mediators in a functional model of decidualized endometrial stromal cells Amélie Bourdiec, Syed-Furquan Ahmad,

Temporal expression pattern of progesterone receptor in the uterine luminal epithelium suggests its requirement during early events of implantation Honglu.

Maternal concentrations of human chorionic gonadotrophin in very early IVF pregnancies and duration of pregnancy: a follow-up study Tom G. Tanbo, Manuela.

Volume 62, Issue 3, Pages (September 2002)

Vascular density and distribution of vascular endothelial growth factor (VEGF) and its receptor VEGFR-2 (Flk-1) are significantly higher in patients with.

Shalmali J. Dharma, M. Sc. , Deepak N. Modi, Ph. D. , Tarala D

Daniel F. Wallace, Lesa Summerville, V. Nathan Subramaniam

Volume 18, Issue 6, Pages (June 2010)

Pathophysiologic features of “thin” endometrium

Expression of the novel gene embryo implantation factor 2 (EMO2) in the mouse uterus at the implantation sites Zhaogui Sun, Ph.D., Renwei Su, Ph.D.,

Decreased expression of FOXA2 promotes eutopic endometrial cell proliferation and migration in patients with endometriosis Anping Lin, Juan Yin, Chao.

Recovery from hind limb ischemia is less effective in type 2 than in type 1 diabetic mice: Roles of endothelial nitric oxide synthase and endothelial.

Extracorporeal shock wave therapy ameliorates secondary lymphedema by promoting lymphangiogenesis Masayuki Kubo, PhD, Tao-Sheng Li, MD, PhD, Takahiro.

Mu opioid receptor in the human endometrium: dynamics of its expression and localization during the menstrual cycle Lide Totorikaguena, B.S., Estibaliz.

Epiphyseal growth plate growth hormone receptor signaling is decreased in chronic kidney disease–related growth retardation Ariel Troib, Daniel Landau,

Dynamics of expression and localization of the cannabinoid system in granulosa cells during oocyte nuclear maturation Ekaitz Agirregoitia, Ph.D., Inés.

Volume 61, Issue 6, Pages (June 2002)

Locally elevated leukemia inhibitory factor in the inflamed fallopian tube resembles that found in tubal pregnancy Yin Fen Ji, M.D., Li You Chen, M.M.,

Pasquale Florio, M. D. , Ph. D. , Paulo B. Torres, M. D

A Role for Estrogen Receptor-α and Estrogen Receptor-β in Collagen Biosynthesis in Mouse Skin Margaret Markiewicz, Sergey Znoyko, Lukasz Stawski, Angela.

Calcitonin receptor isoforms expressed in the developing rat kidney

Volume 8, Issue 3, Pages (August 2014)

Effect of Tenascin-C Deficiency on Chemically Induced Dermatitis in the Mouse Yoh-ichi Koyama, Masashi Kusubata, Atsushi Yoshiki, Noriko Hiraiwa, Tomoo.

Zu-Lin Chen, Sidney Strickland Cell

Jing Li, Ph. D. , Wei-Min Liu, Ph. D. , Yu-Jing Cao, M. S

Volume 18, Issue 6, Pages (June 2010)

Microvascular density, vascular endothelial growth factor A, and its receptors in endometrial blood vessels in patients with menorrhagia Miriam Mints,

Recovery from hind limb ischemia is less effective in type 2 than in type 1 diabetic mice: Roles of endothelial nitric oxide synthase and endothelial.

Effect of ovarian steroids on gene expression profile in human uterine microvascular endothelial cells Tadahiro Yasuo, M.D., Kotaro Kitaya, M.D. Fertility.

Volume 72, Issue 11, Pages (December 2007)

Upregulation of mRNA expression of vascular endothelial growth factor and its receptors by exogenous human chorionic gonadotropin in cultured oviduct.

Volume 58, Issue 6, Pages (December 2000)

Matrix Metalloproteinase Inhibitor BB-3103 Unlike the Serine Proteinase Inhibitor Aprotinin Abrogates Epidermal Healing of Human Skin Wounds Ex Vivo1

Presentation transcript:

Differential expression of vascular endothelial growth factor-A isoforms in the mouse uterus during early pregnancy Lisa M. Walter, Peter A.W. Rogers, Jane E. Girling Reproductive BioMedicine Online Volume 21, Issue 6, Pages 803-811 (December 2010) DOI: 10.1016/j.rbmo.2010.07.005 Copyright © 2010 Terms and Conditions

Figure 1 Uterine vascular endothelial growth factor (VEGF)-A isoform mRNA expression in CBA/C57 mice from day 1 to 5 of pregnancy. The mRNA expression of (A) total Vegf-A, (B) Vegf120, (C) Vegf164 and (D) Vegf188 in pregnant mice was normalized against 18S rRNA. Data is expressed as the fold change compared with the median value on day 1 of pregnancy. The lines in the boxes represent the medians and the boxes extend from the 25th to the 75th centiles. The error bars indicate the minimum and maximum values (n=6). Groups that do not share a letter in common are significantly different (P<0.05). Reproductive BioMedicine Online 2010 21, 803-811DOI: (10.1016/j.rbmo.2010.07.005) Copyright © 2010 Terms and Conditions

Figure 2 Uterine vascular endothelial growth factor (VEGF)-A receptor mRNA expression in CBA/C57 mice from day 1 to 5 of pregnancy. The mRNA expression of (A) Vegfr-2, (B) Nrp-1 and (C) Nrp-2 in pregnant mice were normalized against 18S rRNA. Data is expressed as the fold change compared with the median value on day 1 of pregnancy. The lines in the boxes represent the medians and the boxes extend from the 25th to the 75th centiles. The error bars indicate the minimum and maximum values (n=6). Groups that do not share a letter in common are significantly different (P<0.05). Reproductive BioMedicine Online 2010 21, 803-811DOI: (10.1016/j.rbmo.2010.07.005) Copyright © 2010 Terms and Conditions

Figure 3 Uterine vascular endothelial growth factor (VEGF)-A protein expression in CBA/C57 mice from day 1 (D1) to 5 (D5) of pregnancy. Representative Western blot analysis of VEGF-A isoforms under reducing conditions. Bands at 20–23kDa and 27kDa represent VEGF164 and VEGF188, respectively. A very pale band was observed at ∼18kDa in some samples (thought to be VEGF120, white arrows). Molecular weight of bands determined by comparison with pre-stained, broad range standards (left-hand side). BC=Breast cancer sample used as positive control. Reproductive BioMedicine Online 2010 21, 803-811DOI: (10.1016/j.rbmo.2010.07.005) Copyright © 2010 Terms and Conditions

Figure 4 Vascular endothelial growth factor receptor (VEGFR)-2 immunostaining in CBA/C57 mouse uterus during early pregnancy. Representative photomicrograph of endometrium from pregnant CBA/C57 mice immunostained with VEGFR-2 antibody. Inset; negative control (rabbit IgG). Black arrows indicate blood vessels; e=luminal epithelium; g=gland; l=uterine lumen; s=stroma. Bar=100 μm. Reproductive BioMedicine Online 2010 21, 803-811DOI: (10.1016/j.rbmo.2010.07.005) Copyright © 2010 Terms and Conditions