ELISA

Serological test Antibodies are specific for the antigen stimulated their production Indirect ELISA Patient serum ahs antibodies again a certain antigen Patient has been expose to that pathogen

Antibodies against HIV Secondary antibodies Against human Antibodies. Neisseria gonorrhoeae Enzyme linked antibodies Substrate – colorless Product – blue

6 patients History suggests they might have picked up HIV. Infects CD4 Virus sometimes becomes latent No symptoms Eventually virus destroys T cells Susceptible to opportunistic infections

Transmitted through Blood Other body fluids (sexual contact) Contaminated needle

1/100 1/102 1/10,000 1/104 1/1000000 1/106 10,000 100,000 1,000,000 10,000000

# microbes in 1g soil = # colonies X reciprocal of the dilution 50 colonies Dilution 1/10,000 (50) (10,000) =500,000 cells/gram of soil