Synthetic Biology. Synthetic Biology About Synthetic Biology “The application of engineering design principles to do construction, characterization,

Slides:

Advertisements

Similar presentations

Repressilator: “I’ll be back.”

Advertisements

Davidson College Synth-Aces Tamar Odle (’08), Oscar Hernandez (’06), Kristen DeCelle (’06), Andrew Drysdale (’07), Matt Gemberling (’06), and Nick Cain.

Selected Case Studies. MIT 2006: Engineering bacteria to smell good BSMT wintergreen C. breweriS. cerevisiae ATF1 banana Slides borrowed from the 2006.

ECOLITASTER Making E. Coli tasting flavors Valencia iGEM 2006.

Figure 4.2 from Royal Academy of Engineering report (June, 2009) “What comes to mind when I say ‘synthetic biology’?”

May 12-15, 2011 (red) May 6-11, 2011 (light red) Permanent Water (blue)

Synthetic Biology Crash Course DAY ONE. Pre-Assessment 1:00-1:10.

Synthetic Biology and iGEM Cambridge iGEM2007 team 27 July 2007.

Parts.mit.edu iGEM & Synthetic Biology International Genetically Engineered Machine Competition Randy Rettberg igem2007.comparts.mit.edu SB 3.0 Zurich.

PROJECT REVIEW BERKELEY 2006: ADDRESSABLE CONJUNCTION IN BACTERIAL NETWORKS Fei Chen.

Goal To engineer a plant that emits light to act as natural street lamps in rural parts of the developing world.

Introduction to Synthetic Biology Dannenberg and Purdy 2012 (Tokos edits 2012)

First Institute of Higher Education in Modern China Since 1895 A World-class, Progressive, and Multidisciplinary Research University.

Synthetic Biology Presentation by Brown iGEM Team.

Wits_CSIR iGEM 2011/iGEM experience Presenter: Gloria Hlongwane School of Molecular and Cell Biology/ School of Chemical and Metallurgical Engineering,

Synthetic Biology Project Examples

BACTO-ART A Multicomponent Inducible Expression System for Teaching and Discovery Project Goals To create a multicomponent, inducible expression system.

By Kyle Albert & Josh Mixdorf. Why  Uncontrolled levels of estrogen in waste water treatment plants is a growing problem.  The high levels of estrogen.

Virginia iGEM Workshop #3 High School Education Series.

QGEM Queen’s Genetically Engineered Machine Team QGEM: Queen’s Genetically Engineered Machine Team Information Session: Jan 21 st 2009.

Genetic Circuit Challenge Workshop Thursday 24 Nov 2011 Bentham seminar room 1.

Workshop Template This is a template presentation which can be adapted for your own school workshop. Try to customise it as much as possible, depending.

Beta Brainstorming Yi Jin & John. 1 Biological Counter Aim Synthetic biology circuit that: senses an event counts how many times the event has occurred.

Core members iGEM Web of registries Based on MIT Registry of Standard Biological Parts (parts.mit.edu)parts.mit.edu Well-characterized, community-rated,

IGEM TEAM EPF-LAUSANNE International Genetically Engineered Machine.

Cling-E. coli : Bacteria on target Harvard iGEM 2007 Ellenor Brown Stephanie Lo Alex Pickett Sammy Sambu Kevin Shee Perry Tsai Shaunak Vankudre George.

Kaifeng Chen Institute for Theoretical Physics Synthetic Biology with Engineering Tools 1 Francis Chen.

IGEM 2010 European Spring Workshop Evry, Paris, France 12 th & 13 th June 2010.

Characterization of the Hrp Regulatory System Project 1 of the Imperial iGEM 2007 team.

Synthetic Biology: Design and Characterization of antiswitches in E.coli Matt Gemberling 27 April 2006.

BioBytes: A high throughput Method for Minimal Genome construction The U of A 2009 iGEM Project:

Cling-E. coli : Bacteria on target Harvard iGEM 2007 Ellenor Brown Stephanie Lo Alex Pickett Sammy Sambu Kevin Shee Perry Tsai Shaunak Vankudre George.

AND Gate Inputs Output Input A (Switch) Input B (Switch) Output Y (Lamp) 0 (Open) 0 (OFF) A B Lamp.

Standardization in Engineering Parts –Categorization –Community agrees on properties –Characterization of function –Quality control Assembly –Creates efficiencies.

Cling-E. coli : Bacteria on target Harvard iGEM 2007 Ellenor Brown Stephanie Lo Alex Pickett Sammy Sambu Kevin Shee Perry Tsai Shaunak Vankudre George.

Recombinant DNA Technology Characterization of transcription regulatory sequences by exploiting reporter genes.

Chapter 8, part B Microbial Genetics.

Synthetic Biology Sergio Peisajovich Lim Lab June 2007.

Brainstorming Session Overview:

Distributed computation: the new wave of synthetic biology devices

Objective: Students will be able to identify the different structures of proteins and their functions. Students will be able identify the limits of enzymes.

What is Synthetic Biology

IGEM Advisors Meeting 4/23/2010.

Project Idea: Biofuels

BioBricks: Registry and Assembly Standarts

Clever/Descriptive Title

Clever/Descriptive Title

Standardized and Scalable DNA Assembly

Definitions and Measures of Performance for Standard Biological Parts

Virginia iGEM Workshop #2 High School Education Series.

Motivation #1: Pathways removed from natural regulation when heterologously expressed

Wendell A. Lim, Connie M. Lee, Chao Tang Molecular Cell

Enzyme Reaction Rates -enzymes are also known as biological catalysts

Electromagnetic Radiation

iGEM 2010 General Update and Project Ideas

Population Differentiation Generator

Biology II iGEM Drylab.

Charles Choi, Michael N. Nitabach Current Biology

Enzymes Biological catalyst – it speeds up reactions without being permanently changed.

Functional RNAs expand the synthetic biological toolbox

Golden Bread Explores the science, engineering and bioethics of a yeast that’s genetically modified to make a vitamin-enriched food. Lab activities include.

Engineering Biological Systems with Synthetic RNA Molecules

Week 4 Energy and Enzymes

Welcome to iGEM 2007!.

Uses and application in the real world

Developed in collaboration with

Presentation transcript:

Synthetic Biology

About Synthetic Biology “The application of engineering design principles to do construction, characterization, and design of biological systems” C. Smolke Engineering design principles such as: Standardization Grammar implementation Design Implementation (CAD)

iGEM & Registry Registry of Standarized Biological Parts: repositorium of BioBrick compliant parts. iGEM: the primary international competition on synthetic biology aimed at enriching and curating the Registry of Biological Parts. Focus at undergraduate programs around the world, with support from recognized advisors

Standards The BioBrick Standard: Prefix Suffix Scalability

WiFi Coli

Goal & Approach Goal: communication while deprecating chemical / spatial barrier Approach: use a non-chemical messenger, i.e. photons Encoding: have wavelength encoding Parts: use existing available machinery

Platform Use BioBrick standard to: assemble BioParts into modules, organize modules into devices, express devices within systems. BBa_I15008 BBa_I15008 BBa_I15009 BBa_I15010 BBa_I15009 BBa_I15010

Platform Use BioBrick standard to: assemble BioParts into modules, organize modules into devices, express devices within systems. BBa_I15008 BBa_I15008 BBa_I15009 BBa_I15010 BBa_I15009 BBa_I15010 Regulator BBa_I15008 BBa_I15009 BBa_I15010 Terminator

Platform Use BioBrick standard to: assemble BioParts into modules, organize modules into devices, express devices within systems. BBa_I15008 BBa_I15008 BBa_I15009 BBa_I15010 BBa_I15009 BBa_I15010 Regulator BBa_I15008 BBa_I15009 BBa_I15010 Terminator

Organization Emission Transmission Reception Reception Module Emission

Constructions

Expanded Behavior Recycling pathway for substrate Inducible expression of enzyme, ergo light

Characterization Reception Module PoPs Photons Emission Module Photons Transfer Function Emission Module Photons PoPs Transfer Function

Applications

Trinary Cycle Red Emit. Blue Recep. Red Recep. Green Emit. Blue Emit. Green Recep.

Control Cycle Rec. Module Emis. Module X

Logic Gates & Processor Input Output

Bacterial Luciferase FireFly Luciferase

LRE Recycling Enzyme

Magic Quirality Conversion