Volume 62, Issue 4, Pages (October 2012)

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Volume 62, Issue 4, Pages 720-727 (October 2012) Both Immediate and Delayed Intracavernous Injection of Autologous Adipose-derived Stromal Vascular Fraction Enhances Recovery of Erectile Function in a Rat Model of Cavernous Nerve Injury Xuefeng Qiu, Thomas M. Fandel, Ludovic Ferretti, Maarten Albersen, Haiyang Zhang, Guiting Lin, Ching-Shwun Lin, Tania Schroeder, Tom F. Lue European Urology Volume 62, Issue 4, Pages 720-727 (October 2012) DOI: 10.1016/j.eururo.2012.02.003 Copyright © 2012 European Association of Urology Terms and Conditions

Fig. 1 Electrostimulation of cavernous nerves (CN) at 12 wk. (a) Representative intracavernous pressure (ICP) recording of each experimental group. The black curve represents ICP values in response to CN stimulation. The red bar represents 50-s electrical stimulation of the CN. (b) The effect of stromal vascular fraction treatment in improving the ICP-to- mean arterial pressure ratio. ICP=intracavernous pressure; MAP=mean arterial pressure. * p<0.05 compared with vehicle group. European Urology 2012 62, 720-727DOI: (10.1016/j.eururo.2012.02.003) Copyright © 2012 European Association of Urology Terms and Conditions

Fig. 2 Neuronal nitric oxide synthase (nNOS) staining in a penile midshaft specimen. Representative images of nNOS-positive fibers in penile dorsal nerve of each experimental group are presented. NNOS-positive fibers were stained red (arrow) in the dorsal nerve. Original magnification is ×400. nNOS=neuronal nitric oxide synthase; DAPI=4’,6-diamidino-2-phenylindole. European Urology 2012 62, 720-727DOI: (10.1016/j.eururo.2012.02.003) Copyright © 2012 European Association of Urology Terms and Conditions

Fig. 3 Neurofilament (NF) staining in a penile midshaft specimen. Representative images are presented for NF-positive fibers in penile dorsal nerve of each experimental group. Original magnification is ×400. NF=neurofilament; DAPI=4’,6-diamidino-2-phenylindole. European Urology 2012 62, 720-727DOI: (10.1016/j.eururo.2012.02.003) Copyright © 2012 European Association of Urology Terms and Conditions

Fig. 4 Masson's trichrome and phalloidin staining in a penile midshaft specimen. (a) Representative images of actin staining of each experimental group. Smooth muscle in corpus cavernosum was stained red. Original magnification is ×40. (b) Representative images of Masson's trichrome staining of each experimental group. Smooth muscle and connective tissue are stained red and green, respectively. Original magnification is ×40. DAPI=4’,6-diamidino-2-phenylindole. European Urology 2012 62, 720-727DOI: (10.1016/j.eururo.2012.02.003) Copyright © 2012 European Association of Urology Terms and Conditions