DNA and Chromosome Replication

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Presentation transcript:

DNA and Chromosome Replication Lecture 9 BSCI 420/421/620 Sept.23, 2002 “Only after the last tree has been cut down. Only after the last river has been poisoned. Only after the last fish has been caught. Only then will you find that money cannot be eaten.” - Cree Indian Prophecy A. Prokaryotic chromosome replication B. DNA synthesis C. Initiation of DNA replication

A. Prokaryotic and organellar chromosome replication 1. These are circular molecules w few proteins 2. 2 specialized regions: a. One origin of replication b. Membrane attachment point. Same? Yes

B. DNA Replication

DNA primase

Lagging strand synthesis

DNA helicase

Single-strand binding proteins

Replication fork in eucaryotes

Moving replication fork

DNA topoisomerases prevent DNA tangling during replication or translation

Topoisomerase 1

Topoisomerase II

C. Initiation of DNA Replication Begins at Origins of Replication

Eukaryotic (yeast) ORI genes

New nucleosome assembly

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