Agilent 1100 Series System Operating principles Pumping principle Sampler principles TCC peltier principle Detector principle Introduction 11/13/2018 ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

Agilent 1100 Series Pump Principles Explain how the pump is designed and where the different components are situated. 11/13/2018 ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

Agilent 1100 Series Pump Principle Explain the differences between the G1311A and G1312A and the difference in delay volume and mixing performance. Stress where the first point of mixing are in the two pumps. 11/13/2018 ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

Agilent 1100 Series Pump Principle LPM Board Common to Iso and Quat Pumps ("Type"? ) Metering Drive Power Supply 1100 Common Part Fan Flow Pulse Damper Common to 1100, HP1050 and HP1090 Pumps Point to the different components of the pump. Pump Head Leak Sensor 1100 Common Part Leak Tray 11/13/2018 ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

Agilent 1100 Series Autosampler Principle Describe the injection cycle. BEWARE the slide is wrong. The line from the valve to the metering should go into the metering head instead. 11/13/2018 ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

Agilent 1100 Series Autosampler Principle Critical Connections, snap down correctly! ASM Board Transport Unit Power Supply Common 1100 Part Sampling Unit Point to the different parts of the sampler. Vial Trays: 100-2ml 40-2ml 15-ml 11/13/2018 ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

Agilent 1100 Series Well Plate Sampler principle Explain the difference of the cycle compared to the Standard sampler. 11/13/2018 ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

Agilent 1100 Series Thermostatted Column Compartment principle Explain the Peltier principle and the use of the two different sides. 11/13/2018 ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

Agilent 1100 Series Variable Wavelength Detector Principle Lamp Optical mirrors Grating Detector cell Slit Beam splitter Explain how the G1314A measures a peak and how the referencing is done. 11/13/2018 ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

Agilent 1100 Series Variable Wavelength Detector Principle VWD Board Lamp housing Fan Power Supply 1100 Common Part Point to the different parts of the VWD. Optical unit Flow cell Leak Sensor 1100 Common Part 11/13/2018 ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

Recommended Flow Cells For Pressure Above 100 bar Explain how to read this slide and why the column ID and flow is interconnected. 11/13/2018 ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

Agilent 1100 Series Diode Array Detector Principle Tungsten lamp Lens Deuterium lamp Lens system Holmium filter Detector cell Optical slit Grating Diode Array Explain how the G1315B measures a peak and how the referencing is done. Explain the difference between the A and the B. 11/13/2018 ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

Agilent 1100 Series Diode Array Detector Principle DAD Board Fan Lamp housing Power Supply 1100 Common Part Point to the different parts of the DAD. Optical unit Flow cell Leak Sensor 1100 Common Part 11/13/2018 ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction

Recommended Flow cells See previous slide. 11/13/2018 ANCE-LC-II-027 AGILENT 1100 Series HPLC Operation, Hardware and OQ/PV, Introduction