Evaluation of the Epstein-Barr Virus R-Gene Quantification Kit in Whole Blood with Different Extraction Methods and PCR Platforms Samira Fafi-Kremer,

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Evaluation of the Epstein-Barr Virus R-Gene Quantification Kit in Whole Blood with Different Extraction Methods and PCR Platforms Samira Fafi-Kremer, Patrice Morand, Come Barranger, Gérard Barguès, Stéphane Magro, Jérôme Bés, Philippe Bourgeois, Martine Joannes, Jean-Marie Seigneurin The Journal of Molecular Diagnostics Volume 10, Issue 1, Pages 78-84 (January 2008) DOI: 10.2353/jmoldx.2008.070054 Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 Analytic sensitivity of the EBV R-gene assay with two different PCR platforms. The EBV R-gene assay used with both PCR platforms, after manual extraction, detected all six replicates of specimen containing 10 copies of EBV DNA per reaction. The Journal of Molecular Diagnostics 2008 10, 78-84DOI: (10.2353/jmoldx.2008.070054) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Correlation of EBV DNA load quantified in EBV-positive whole-blood samples (n = 126) by the EBV R-gene quantification kit in the SmartCycler 2.0 and in-house PCR in the LightCycler 1.0, after automated extraction by the MagNAPure LC (r = 0.92; P < 0.0001). The Journal of Molecular Diagnostics 2008 10, 78-84DOI: (10.2353/jmoldx.2008.070054) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 Correlation of longitudinal EBV DNA load dynamics measured by the EBV R-gene quantification kit and in-house PCR in two patients at risk for EBV-associated diseases (patients A and B). GVHD, graft-versus-host reaction disease; ATG, antithymocyte globulin. The Journal of Molecular Diagnostics 2008 10, 78-84DOI: (10.2353/jmoldx.2008.070054) Copyright © 2008 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions