Activation of mast cells by double-stranded RNA: evidence for activation through Toll- like receptor 3 Marianna Kulka, PhD, Lena Alexopoulou, PhD, Richard.

Slides:

Advertisements

Similar presentations

Secretory IgA induces antigen-independent eosinophil survival and cytokine production without inducing effector functions Kathleen R. Bartemes, BA, Kate.

Advertisements

Proinflammatory cytokine–induced and chemical mediator–induced IL-8 expression in human bronchial epithelial cells through p38 mitogen-activated protein.

Maria B. Sukkar, PhD, Shaoping Xie, PhD, Nadia M

IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,

Regulation of TH2 responses by the pulmonary Clara cell secretory 10-kd protein Chih-Hsing Hung, MD, Li-Chen Chen, MD, Zhongjian Zhang, PhD, Bhabadeb.

Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.

Thymic stromal lymphopoietin downregulates filaggrin expression by signal transducer and activator of transcription 3 (STAT3) and extracellular signal-regulated.

Factors affecting the cytokine production of human T cells stimulated by different modes of activation Yoshio Harada, MDa, Sumiko Watanabe, PhDa, Hans.

Regulation of Mast Cell Survival by IgE

Signal transduction pathways triggered by the FcϵRIIb receptor (CD23) in human monocytes lead to nuclear factor-κB activation Rosa M. Ten, MD, PhDa,

Ting-ting Zhang, MSc, Klaus Okkenhaug, PhD, Baher F

Increased expression of nuclear factor of activated T cells 1 drives IL-9–mediated allergic asthma Sonja Koch, PhD, Anna Graser, PhD, Hooman Mirzakhani,

Ramses Ilarraza, PhD, Yingqi Wu, Christopher D

FcεRI-mediated amphiregulin production by human mast cells increases mucin gene expression in epithelial cells Shigeru Okumura, DDS, PhD, Hironori Sagara,

Targeting Toll-like receptors on dendritic cells modifies the TH2 response to peanut allergens in vitro Pierre Pochard, PhD, Brian Vickery, MD, M. Cecilia.

Interleukin-25 induced by human chorionic gonadotropin promotes the proliferation of decidual stromal cells by activation of JNK and AKT signal pathways

Glycation of a food allergen by the Maillard reaction enhances its T-cell immunogenicity: Role of macrophage scavenger receptor class A type I and II

Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.

LPS induces CD40 gene expression through the activation of NF-κB and STAT-1α in macrophages and microglia by Hongwei Qin, Cynthia A. Wilson, Sun Jung Lee,

Evidence of a role for B cell–activating factor of the TNF family in the pathogenesis of chronic rhinosinusitis with nasal polyps Atsushi Kato, PhD,

Surfactant protein D inhibits TNF-α production by macrophages and dendritic cells in mice László Hortobágyi, MS, Sonja Kierstein, PhD, Kateryna Krytska,

Dawn C. Newcomb, PhD, Madison G

The anti-inflammatory effect of glucocorticoids is mediated by glucocorticoid-induced leucine zipper in epithelial cells Jane Eddleston, PhD, Jack Herschbach,

Cysteinyl leukotriene E4 activates human group 2 innate lymphoid cells and enhances the effect of prostaglandin D2 and epithelial cytokines Maryam Salimi,

Human IL-31 is induced by IL-4 and promotes TH2-driven inflammation

Toll-like receptor 9 suppression in plasmacytoid dendritic cells after IgE-dependent activation is mediated by autocrine TNF-α John T. Schroeder, PhD,

Toll-like receptor 3 enhances late-phase reaction of experimental allergic conjunctivitis Mayumi Ueta, MD, PhD, Satoshi Uematsu, MD, PhD, Shizuo Akira,

Increased expression of CC chemokine ligand 18 in patients with chronic rhinosinusitis with nasal polyps Sarah Peterson, MD, Julie A. Poposki, MS, Deepti.

Mite allergen is a danger signal for the skin via activation of inflammasome in keratinocytes Xiuju Dai, MD, PhD, Koji Sayama, MD, PhD, Mikiko Tohyama,

Miriam Wittmann, MD, Jana Zeitvogel, Dong Wang, MD, Thomas Werfel, MD

Corticosteroid insensitivity of chemokine expression in airway smooth muscle of patients with severe asthma Po-Jui Chang, MD, Pankaj K. Bhavsar, PhD,

Dexamethasone regulation of thymic stromal lymphopoietin receptor expression on mast cells and their precursors Zoulfia Allakhverdi, PhD, Michael R.

Interleukin receptor-associated kinase-4 deficiency impairs Toll-like receptor–dependent innate antiviral immune responses Douglas R. McDonald, MD, PhD,

Toll-like receptor 9, transmembrane activator and calcium-modulating cyclophilin ligand interactor, and CD40 synergize in causing B-cell activation Esra.

Inhibition of human B-cell development into plasmablasts by histone deacetylase inhibitor valproic acid Anne-Kathrin Kienzler, MSc, Marta Rizzi, MD,

Estrogen increases the severity of anaphylaxis in female mice through enhanced endothelial nitric oxide synthase expression and nitric oxide production

Role of hyaluronan and hyaluronan-binding proteins in human asthma

Zoulfia Allakhverdi, PhD, Michael R. Comeau, BSc, Dirk E

Regulation of Mast Cell Survival by IgE

Exogenous IFN-β has antiviral and anti-inflammatory properties in primary bronchial epithelial cells from asthmatic subjects exposed to rhinovirus Julie.

Thymic stromal lymphopoietin as a mediator of crosstalk between bronchial smooth muscles and mast cells Zoulfia Allakhverdi, PhD, Michael R. Comeau,

Thymic stromal lymphopoietin activity is increased in nasal polyps of patients with chronic rhinosinusitis Deepti R. Nagarkar, PhD, Julie A. Poposki,

Notch signaling confers antigen-presenting cell functions on mast cells Nobuhiro Nakano, PhD, Chiharu Nishiyama, PhD, Hideo Yagita, PhD, Akemi Koyanagi,

The relative contribution of IL-4 and IL-13 to human IgE synthesis induced by activated CD4+ or CD8+ T cells Juha Punnonen, MD, PhD, Hans Yssel, PhD,

C5a Negatively Regulates Toll-like Receptor 4-Induced Immune Responses

Abrogation of TGFβ Signaling in T Cells Leads to Spontaneous T Cell Differentiation and Autoimmune Disease Leonid Gorelik, Richard A Flavell Immunity

UVB and Proinflammatory Cytokines Synergistically Activate TNF-α Production in Keratinocytes through Enhanced Gene Transcription Muhammad M. Bashir,

IL-18 Downregulates Collagen Production in Human Dermal Fibroblasts via the ERK Pathway Hee Jung Kim, Seok Bean Song, Jung Min Choi, Kyung Moon Kim,

Josée Lamoureux, PhD, Jana Stankova, PhD, Marek Rola-Pleszczynski, MD

IL-12 affects Dermatophagoides farinae–induced IL-4 production by T cells from pediatric patients with mite-sensitive asthma Takeshi Noma, MD, PhD, Izumi.

Monocyte-derived dendritic cell recruitment and allergic TH2 responses after exposure to diesel particles are CCR2 dependent Sharen Provoost, MSc, Tania.

Prostaglandin D2 activates group 2 innate lymphoid cells through chemoattractant receptor-homologous molecule expressed on TH2 cells Luzheng Xue, PhD,

Lipoteichoic acid downregulates FcεRI expression on human mast cells through Toll-like receptor 2 Mino Yoshioka, MSc, Nobuyuki Fukuishi, PhD, Sayuri.

IL-17E upregulates the expression of proinflammatory cytokines in lung fibroblasts Séverine Létuvé, PhD, Stéphane Lajoie-Kadoch, MSc, Séverine Audusseau,

Amplification of Toll-like receptor–mediated signaling through spleen tyrosine kinase in human B-cell activation Shigeru Iwata, MD, PhD, Kunihiro Yamaoka,

Volume 17, Issue 3, Pages (September 2002)

MicroRNA-4443 regulates mast cell activation by T cell–derived microvesicles Irit Shefler, PhD, Pazit Salamon, PhD, Francesca Levi-Schaffer, PharmD, Adam.

Fetal and neonatal IL-13 production during pregnancy and at birth and subsequent development of atopic symptoms Toby J. Williams, Catherine A. Jones,

Toll-like receptor 2 ligands activate human basophils for both IgE-dependent and IgE- independent secretion Anja P. Bieneman, BS, Kristin L. Chichester,

The steroidogenic enzyme Cyp11a1 is essential for development of peanut-induced intestinal anaphylaxis Meiqin Wang, MD, PhD, Julita Ramirez, DVM, PhD,

The novel flavone tetramethoxyluteolin is a potent inhibitor of human mast cells Zuyi Weng, MS, PhD, Arti B. Patel, MS, Smaro Panagiotidou, MA, Theoharis.

Toll-like receptor agonists differentially regulate cysteinyl-leukotriene receptor 1 expression and function in human dendritic cells Maryse Thivierge,

Regulation of IL-13 receptor α1 expression and signaling on human tonsillar B- lymphocyte subsets Oumnia Hajoui, PhD, Huaien Zheng, MD, PhD, Julie Guay,

Heterozygous N-terminal deletion of IκBα results in functional nuclear factor κB haploinsufficiency, ectodermal dysplasia, and immune deficiency Douglas.

Zhenying Nie, PhD, Cole S. Nelson, PhD, David B. Jacoby, MD, Allison D

Julie Negri, BA, S. Brandon Early, BA, John W

Induced CD4+ forkhead box protein–positive T cells inhibit mast cell function and established contact hypersensitivity through TGF-β1 Wenru Su, MD, Huimin.

Ramses Ilarraza, PhD, Yingqi Wu, Christopher D

Intact IL-12 signaling is necessary for the generation of human natural killer cells with enhanced effector function after restimulation Venkateswara.

Abrogation of TGFβ Signaling in T Cells Leads to Spontaneous T Cell Differentiation and Autoimmune Disease Leonid Gorelik, Richard A Flavell Immunity

Presentation transcript:

Activation of mast cells by double-stranded RNA: evidence for activation through Toll- like receptor 3 Marianna Kulka, PhD, Lena Alexopoulou, PhD, Richard A Flavell, PhD, Dean D Metcalfe, MD Journal of Allergy and Clinical Immunology Volume 114, Issue 1, Pages 174-182 (July 2004) DOI: 10.1016/j.jaci.2004.03.049

Fig 1 A, RT-PCR analysis of IFN-α/β mRNA production after dsRNA (polyI:C, 10 μg/mL) treatment for 0 to 8 hours. B, Real-time PCR analysis of IFN-α gene expression in HCMCs treated with dsRNA (polyI:C, 10 μg/mL). C, ELISA analysis of IFN-α production from HUMCs stimulated with dsRNA (polyI:C, 10 μg/mL). D, Flow cytometry analysis of IFN-α production after HCMC stimulation with polyI:C, polyA:U, polyC:G, polyC, and polydI:dC (all at 10 μg/mL) for 24 hours (n=3). Journal of Allergy and Clinical Immunology 2004 114, 174-182DOI: (10.1016/j.jaci.2004.03.049)

Fig 2 TLR expression in HMC-1, HCMC (at 4 weeks and 8 weeks of culture), and LAD cells determined by RT-PCR (A) and Western blot analysis (B). Human spleen RNA and protein lysate were used as a positive control. Journal of Allergy and Clinical Immunology 2004 114, 174-182DOI: (10.1016/j.jaci.2004.03.049)

Fig 3 TLR-3 expression on BMMCs. Bone marrow aspirate mononuclear cell fraction was incubated with anti-TLR3-PE (5 μg/mL) and anti—Kit–antigen-presenting cells (2 μg/mL) for 30 minutes at 4°C and analyzed on a FACSCalibur. Journal of Allergy and Clinical Immunology 2004 114, 174-182DOI: (10.1016/j.jaci.2004.03.049)

Fig 4 A, HCMCs were incubated with 5 μg/mL IgG isotype control, anti–TLR-2, anti–TLR-3 (clone Q-18), anti–TLR-3 (clone L-13), and anti–TLR-4 for 1 hour, then stimulated with polyI:C (10 μg/mL) for 24 hours. BMMCs from wild-type and TLR-3 knockout mice were stimulated with polyI:C (B), LPS (C), and antigen (D) for 24 hours, and IFN-α or TNF was measured in supernatants with ELISA (n=3 mice). untr, Untreated. Journal of Allergy and Clinical Immunology 2004 114, 174-182DOI: (10.1016/j.jaci.2004.03.049)

Fig 5 HCMCs were stimulated with polyI:C (10 μg/mL), LPS (100 ng/mL), peptidoglycan (10 μg/mL), CpG-A oligonucleotides (1 μg/mL), flagellin (100 ng/mL), antigen (Ag; 100 ng/mL), or polyI:C+antigen for 24 hours. IFN-α (A), TNF (B), IL-5 (C), IL-1β (D), GM-CSF (E), and CysLT (F) were measured in cell-free supernatants by using ELISA. ∗Statistical significance between untreated and treated (P < .05 compared with untreated, n=3). Journal of Allergy and Clinical Immunology 2004 114, 174-182DOI: (10.1016/j.jaci.2004.03.049)

Fig 6 A, IFN-α production is mediated by JNK and NF-κB activation. HCMCs were stimulated with polyI:C (10 μg/mL) in the presence of p38 JNK inhibitor (SP600125; 0.1 mmol/L), NF-κB inhibitor (SN50; 50 μg/mL), p38 inhibitor (SB202190; 30 μg/mL), or actinomycin-D (5 μg/mL) for 24 hours. IFN-α production was measured by ELISA in cell-free supernatants (n=3). B, HCMCs were treated with polyI:C (10 μg/mL) for 0 to 60 minutes, and cell lysates were examined for the presence of phosphorylated JNK, IκB, and p38 by using phospho-specific mAb. Blots were stripped and reprobed with antiactin mAb to monitor loading (n=3). Journal of Allergy and Clinical Immunology 2004 114, 174-182DOI: (10.1016/j.jaci.2004.03.049)