Vitamin D supplementation during pregnancy: Effect on the neonatal immune system in a randomized controlled trial Eve Hornsby, PhD, Paul E. Pfeffer,

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Presentation transcript:

Vitamin D supplementation during pregnancy: Effect on the neonatal immune system in a randomized controlled trial Eve Hornsby, PhD, Paul E. Pfeffer, MRCP, PhD, Nancy Laranjo, BA, William Cruikshank, PhD, Marina Tuzova, PhD, Augusto A. Litonjua, MD, MPH, Scott T. Weiss, MD, MS, Vincent J. Carey, PhD, George O'Connor, MD, MS, Catherine Hawrylowicz, PhD Journal of Allergy and Clinical Immunology Volume 141, Issue 1, Pages 269-278.e1 (January 2018) DOI: 10.1016/j.jaci.2017.02.039 Copyright © 2017 The Authors Terms and Conditions

Journal of Allergy and Clinical Immunology 2018 141, 269-278 Journal of Allergy and Clinical Immunology 2018 141, 269-278.e1DOI: (10.1016/j.jaci.2017.02.039) Copyright © 2017 The Authors Terms and Conditions

Fig 1 Effect of vitamin D3 supplementation during pregnancy on innate and mitogenic cytokine responses. A, Heat map of ratios of geometric mean cytokine concentrations adjusted for medium control cytokine level. Ratios of greater than 1 (red scale) are indicative of a higher concentration in the 4400 IU/d group. B, Raw cytokine levels. Gray filled symbols, 4400 IU/d group (n = 14-18); open symbols, 400 IU/d group (n = 15-16). There were 2 biological replicates per CBMC sample. Journal of Allergy and Clinical Immunology 2018 141, 269-278.e1DOI: (10.1016/j.jaci.2017.02.039) Copyright © 2017 The Authors Terms and Conditions

Fig 2 Effect of vitamin D supplementation in pregnancy on expression of TLR2, TLR4, and TLR9 (A) and the glucocorticoid receptor (GR) in CBMCs (B). Expression levels were measured by using quantitative PCR and are expressed as 2−ΔCT × 106 relative to 18s. One biological replicate for each participant is shown (n = 22-25 per group). Gray filled circles, 4400 IU/d; open circles, 400 IU/d. Journal of Allergy and Clinical Immunology 2018 141, 269-278.e1DOI: (10.1016/j.jaci.2017.02.039) Copyright © 2017 The Authors Terms and Conditions

Fig 3 Effect of vitamin D supplementation in pregnancy on neonatal cytokine responses after T-cell stimulation. CBMCs were cultured with anti-CD3 and IL-2. Supernatants were harvested at 72 hours, and production of 5 T-cell cytokines was measured. Two biological replicates for each participant are shown. Gray filled circles, 4400 IU/d; open circles, 400 IU/d. Journal of Allergy and Clinical Immunology 2018 141, 269-278.e1DOI: (10.1016/j.jaci.2017.02.039) Copyright © 2017 The Authors Terms and Conditions

Fig 4 Effect of vitamin D supplementation on neonatal responses to dexamethasone. CBMCs were cultured with anti-CD3 and IL-2 for 72 hours in the presence or absence of 1 × 10−7 mol/L dexamethasone (DEX). Change in cytokine concentration in dexamethasone-treated versus untreated cultures is expressed as a log ratio. Two biological replicates per participant are plotted. Gray filled circles, 4400 IU/d; open circles, 400 IU/d. Journal of Allergy and Clinical Immunology 2018 141, 269-278.e1DOI: (10.1016/j.jaci.2017.02.039) Copyright © 2017 The Authors Terms and Conditions