Isolation and Identification of Fungi

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Presentation transcript:

Isolation and Identification of Fungi

Introduction Fungi can be isolated from virtually any place on earth, from the tropics to the poles and from the mountain tops to the deep oceans.

In our lab we will focusing on Air- borne fungi Soil fungi Infected plant Water fungi.

Air- borne fungi As we know fungi are present in air in spore shape but sometimes we find hyphae in air. The mainly function of spores is conserve of species and distribution.

Factors affecting on the distribution of fungi : Temperature. Speed of air. Rains. Humidity. Cycle of spores production

Method of exposing the Petri dish Is the simplest method for obtaining of spores from air is to expose Petri dish with nutrient media to air for a period of time then incubate the plate at room temperature.

Soil Fungi There are many techniques for studying soil fungi and the chose of method are based on the aim of studying. To collect the sample you must take 10- 15 cm of soil approximately 50- 100 gm by clean trowel then store the sample in clean bag.

Studying of soil fungi Direct method Indirect method

Direct method As a result of non- transparent soil component and randomly distribution of spores; its impossible to study the soil directly under the light microscope, so there are simples methods for this aim as :

Using of Contact Slide Method: for studying the natural distribution of in soil. take clean glass slides and put them in different site in soil for one week after that bring the slides, dry, fix them by flame then wash the slid and finally staining these slides for microscopic examination. This method using for studying the distribution of hyphae in soils but its not useful for identification the types of fungi due to not available of spores. Soil Smear method by using clean glass slides and press it in soil then stain these slides with lactophenol- Cotton Blue.

Indirect method Dilution plate method Soil plate method Strewing 0.05 g of soil on nutrient media complementary with antibiotics

Isolation from Infected Plant

Isolation of fungi from contaminated water

Sub culturing make a subculture from old colonies of fungi as follows: Obtain a metal loop or needle and flame it until it turns red hot. Wait for about 10 seconds for the loop or needle to cool down. Using the loop or needle according to which you will transfer, remove an isolated colony of fungi. Aseptically transfer the cell colony into the nutrient media. Incubate the plate at room temperature for the next week.