Nilesh Dighe and David M. Stelly

Slides:



Advertisements
Similar presentations
Fall 2014 HORT6033 Molecular Plant Breeding INSTRUCTOR: AINONG SHI HORT6033 web site:
Advertisements

Identification of AFLP markers linked to tomato spotted wilt virus resistance in tobacco NC STATE UNIVERSITY H. Moon and J.S. Nicholson Department of Crop.
Why do non-Mendelian patterns occur? (When to decide whether it is “Mendelian” or “non- Mendelian”)
Potato Mapping / QTLs Amir Moarefi VCR
Resistance to powdery mildew in wheat germplasm with different resistance sources L. M. Miranda, J. P. Murphy, D. S. Marshall and S.Leath NC STATE UNIVERSITY.
Association Mapping as a Breeding Strategy
Genetic Linkage and Mapping Notation — ————— A _________ A a Aa Diploid Adult Haploid gametes (single chromatid) — ————— Two homologous chromosomes,
Chapter 10, Genetics.
Fundamentals of Genetics
Inheritance of seed coat color of Ethiopian mustard (Brassica carinata A. Braun) Mukhlesur Rahman, Muhammad Tahir 1 Department of Plant Science, North.
Linkage genes and genetic recombination
Backcross Breeding.
Chapter 9: Genetic linkage and maps in breeding applications
Linkage Genes linked on the same chromosome may segregate together.
Symbols to Know for Crosses a/a – a is the allele and / represents the two chromatids – there are two alleles for a diploid organism a b/a b – two different.
Creating a heterozygous male Hermaphrodite Parent genotype Male Parent genotype X Male gametes Female gametes Progeny genotype Which progeny are you continuing.
Genetica per Scienze Naturali a.a prof S. Presciuttini 1. Mendel’s dihybrid crosses Mendel went on to analyze the descendants of pure lines that.
PLANT BIOTECHNOLOGY & GENETIC ENGINEERING (3 CREDIT HOURS)
TOPIC FOUR: INHERITANCE OF A SINGLE GENE Why can’t we all just get along and, say, call an inbred line in the F 6­ generation simply ‘an F 6 line’? Well.
Genetic Mapping Oregon Wolfe Barley Map (Szucs et al., The Plant Genome 2, )
Comparative Performance of BARBREN and LONREN David B. Weaver Professor Department of Agronomy and Soils Auburn University AL Dallas, TX, April 4,
Genotyping and association analysis of Gossypium hirsutum lines for resistance in Reniform Nematodes Megha V. Sharma*, Stella Kantartzi, David Weaver,
What is the probability of rolling a 1 and 5 in two separate rolls? Agenda for Tuesday March 30 th Mendel notes Punnett Squares.
Experimental Design and Data Structure Supplement to Lecture 8 Fall
Characteristics of a population Genotype frequency--the relative proportion of different genotypes in a population with respect to a given locus.
Cotton Breeding At Auburn University
Chapter 10 Introduction to Genetics. Why Study Heredity ? Studying heredity allows us to figure out what our children may look like… Studying heredity.
1 Genetic Mapping Establishing relative positions of genes along chromosomes using recombination frequencies Enables location of important disease genes.
Step one Two gene loci: A & B What will your first cross be in an experiment to test for possible meiotic crossing over? Hint: what condition do you have.
Predicting Inheritance
GENERAL GENETICS Ayesha M. Khan Spring Linkage  Genes on the same chromosome are like passengers on a charter bus: they travel together and ultimately.
Overview What is Plant Breeding? Basic Genetics Mendelian Genetics
Chapter 10 Section 2 Mendelian Genetics. How Genetics Began In 1866, Mendel published the paper "Experiments in Plant Hybridization" studied seven basic.
Mendel’s Pea Plant Experiments
Introduction to Genetics and Heredity
13/11/
Spring 2009: Section 4 – Lecture 1
Association between SSR markers and
The Punnett Square Approach and Probability
Extra Credit Question Crossing over in Eukaryotes occurs during when there are _____ chromatids present at the metaphase plate in Meiosis I, but the crossover.
Gene Mapping in Eukaryotes
CH 10 Mendel and Meiosis.
Chapter 10, Genetics.
Genetics Basics (10.2) State Standard
The authors thank Embrapa for financial support.
PRINCIPLES OF CROP PRODUCTION ABT-320 (3 CREDIT HOURS)
11-2 Probability and Punnett Squares
The Chromosomal Basis of Inheritance GENE MAPPING AP Biology/ Ms. Day
Genetics Notes #2 Mendel & Probability
Bio.B.2- Genetics CHAPTER 11.
Backcross Breeding.
Genetics Basics (10.2) State Standard
The Chromosomal Basis of Inheritance
Heredity and Genetics.
Mapping Quantitative Trait Loci
Linkage, Recombination, and Eukaryotic Gene Mapping
Introduction to Genetics
Mendel laid the groundwork for genetics.
What controls the inheritance of traits in organisms?
10.2 Mendelian Genetics Genetics – The science of heredity.
Sexual Reproduction and Genetics
Selective Plant Breeding
The Chromosomal Basis of Inheritance GENE MAPPING AP Biology/ Ms. Day
Creating a heterozygous male
Genetics Practice.
Fundamental Genetics.
Introduction to Genetics
V. Kyaligonza, R. Kawuki, M. Ferguson, Y. Baguma, T. Kaweesi , J
Symbols to Know for Crosses
Presentation transcript:

Nilesh Dighe and David M. Stelly Recombination of DNA Markers for Reniform and Root-knot Nematode Resistance in LONREN x Acala NemX Hybrids Alois A. Bell USDA-ARS, College Station, TX Nilesh Dighe and David M. Stelly Dept. Soil and Crop Sciences, Texas A&M University, College Station, TX

from G. longicalyx (F) into G. hirsutum (AD) Creation of Triple-Species Tetraploid Hybrids Used to Introgress Resistance from G. longicalyx (F) into G. hirsutum (AD) 1. HLA hybrid (strategy: substitute F for A) AADD x FF (FAD)2 x DD (FADD) 2. HHL hybrid (strategy: substitute F for D) AADD x AA (AAD)2 x FF (AADF) DD = G. armourianum AA = G. herbaceum

LONREN Backcross-1 Families 1. HLA-A Group (4 random BC1 male parents) 8 families derived from backcross-1 plants: 2, 77A, 83, 84*, 85, 103A, 122, and 132 2. HLA-B Group (Acala NemX BC1 male parent) 14 families derived from backcross-1 plants: 4, 8, 21, 26, 34, 35, 45, 61, 75, 77B, 81, 91, 99, 103B 3. HHL Group (Acala NemX BC1 male parent) 6 families derived from backcross plants: 3, 5, 7, 11, 14, and 17 * LONREN-1 and -2 germplasm lines released from this family in April 2007.

Effects of Longicalyx Resistance in Deltapine 458 B/R Isolines Reniform Nematode Populations in BC6S1 Progeny Seven Weeks After Inoculation Resistance Status* n Nematode Numbers (% DP 16) Mean Range RR 12 0.8 0 – 2 Rr 14 3.7 0 – 16 rr 8 106.1 5 – 201 * Determined from test crosses and six SSR markers.

Seed Cotton Yields of LONREN Progeny Rows Texas A&M Brazos River Plantation (2007) Line / Cultivar No. of Rows* Yield Mean Range (Kg/Row) LONREN-1 (Composite of BC7S2 seed from 17 Sibs): Susceptible Sibs 3 3.34 2.80-3.51 Resistant Sibs 16 3.22 2.21-4.12 LONREN-2 (Composite of BC7S2 seed from 20 Sibs): 3.42 3.26-3.70 20 3.75 2.27-4.81 Fibermax 958 6 3.19 2.00-3.65 PSC 355 1 3.65 * 17 Sib lines exceeded the mean of Fibermax 958, the last backcross parent; 10 Sibs exceeded the mean for PSC 355, the performance standard.

Objectives 1. Combine resistance to reniform nematodes from LONREN lines with resistance to root knot nematodes from Acala NemX in agronomically desirable germplasm lines. 2. Develop additional LONREN germplasm lines from other families in the HLA-A group. 3. Develop new sources of root knot resistance with improved yield and fiber quality.

Parents Used in Crosses LONREN Parents: BC6 plant from MB Line (77A family) BC6 plant from MD Line (83 family) BC7 plant from ME Line (84 family) BC6 plant from ML Line (103A family) BC6 plant from MM Line (122 family) BC6 plant from MO Line (132 family) Acala NemX Parents: Type 1 Atypical CIR 316 and BNL 1231 and typical BNL 1066 markers Type 2 Atypical CIR 316 and BNL 1066 and typical BNL 1231 markers

Fiber Quality of CIR 316_202 Sources Cultivar/Line MIC UHM STR UI Acala NemX-1 4.7 1.17 33.9 87.5 Acala Nemx-2 4.9 1.22 33.2 87.2 Auburn 623 5.3 1.11 29.5 85.3 M-315 4.5 1.13 29.3 86.0 STV 5599 5.7 1.16 29.6 Clevewilt 6 3.9 1.24 28.6

Markers on Long Arm of Chromosome 11 Used to Characterize Parents and Follow Crosses Acala NemX LONREN Lines MD, ME, MM, MO Type 1 Type 2 fzg (white) fzg (white) Fzglon (green) Mi2 CIR 316_202 CIR 316_202 CIR 316_191 rkn1 ML cut Area of Possible Recombination Rrlon BNL 1231_197 BNL 1231_192 BNL 1231_null BNL 3279_112 BNL 3279_112 BNL 3279_114 MB cut BNL 1066_130 BNL 1066_139 BNL 1066_156 BNL 836_207 BNL 836_207 BNL 836_215

Markers on Long Arm of Chromosome 11 Used to Characterize Parents and Follow Crosses Acala NemX LONREN Lines MD, ME, MM, MO Type 1 Type 2 fzg (white) fzg (white) Fzglon (green) Mi2 CIR 316_202 CIR 316_202 CIR 316_191 rkn1 ML cut Area of Possible Recombination Rrlon BNL 1231_197 BNL 1231_192 BNL 1231_null BNL 3279_112 BNL 3279_112 BNL 3279_114 MB cut BNL 1066_130 BNL 1066_139 BNL 1066_156 BNL 836_207 BNL 836_207 BNL 836_215

Marker BNL 3279 Scan on 34 BC6S1 Plants (Coupling phase linkage) bp 114 bp 112 D HLA F1 AD1 A Homozygous Resistant Heterozygous Resistant Susceptible Marker BNL 1231 Scan on 34 BC6S1 Plants (Repulsion phase linkage) AD1 D M F1 HLA Homozygous Resistant Heterozygous Resistant Susceptible bp 197 bp 192 B

BNL1231 Profile of 20 Random Acala NemX Plants from USDA Cotton Germplasm Collection Type 1 bp 197 bp 192 Type 2

Seed Showing Effect of Fzglon Gene on Linter Color G. longicalyx fzglon fzglon Fzglon fzglon Fzglon Fzglon

Protocols 1. F1 hybrids heterozygous for BNL 3279_114 and CIR 316_202 were selected for selfing. Then F2 progeny were screened for BNL 3279, CIR 316, BNL 1066, and BNL 1231 markers to identify recombinants. 2. F2 recombinants were selfed to produce F3 generations and were backcrossed to produce BC1 and BC2 generations. 3. Plants homozygous for BNL3279_114 only, CIR 316_202 only, or both are being evaluated for agronomic performance and resistance to nematodes.

Segregation of F2 Progeny LONREN x Acala NemX F1 Genotype LONREN Parent Line Observed Total Theoretical Unlinked 3279* 316* MB MD ME ML MM MO AA BB 18 Bb 1 2 4 36 bb 8 9 13 7 15 65 Aa 3 5 Bb† 25 26 20 23 144 172 aa 11 12 64 * A = BNL 3279_114; a = BNL 3279_112; B = CIR 316_202 from Acala NemX compared to b = CIR 316_191 in LONREN lines. † Parental genotype

Segregation of F2 Progeny LONREN x Acala NemX F1 Genotype LONREN Parent Line Observed Total Theoretical Unlinked 3279* 316* MB MD ME ML MM MO AA BB 18 Bb 1 2 4 36 bb 8 9 13 7 15 65 Aa 3 5 Bb† 25 26 20 23 144 172 aa 11 12 64 * A = BNL 3279_114; a = BNL 3279_112; B = CIR 316_202 from Acala NemX compared to b = CIR 316_191 in LONREN lines. † Parental genotype

(No. F3 Progeny with Genotype) Segregation of F3 Progeny from LONREN x Acala NemX F2 Recombinants (AABb) Genotype* F2 Recombinant Parent 3279 316 ME-25 ML-20 ML-37 MO-33 (No. F3 Progeny with Genotype) AA BB 5 4 8 Bb 28 10 20 bb 7 6 12 Chi Square (1:2:1) 6.6 2.4 0.4 0.8 P Value 0.04 0.30 0.82 0.46 * A = BNL 3279_114; a = BNL 3279_112; B = CIR 316_202; and b = CIR 316_191 markers.

(No. F3 Progeny with Genotype) Segregation of F3 Progeny from LONREN x Acala NemX F2 Recombinants (AaBB) Genotype* F2 Recombinant Parent 3279 316 ML-24 ML-42 ML-43 MM-9 (No. F3 Progeny with Genotype) AA BB 6 3 4 Aa 8 7 27 aa 9 Chi Square (1:2:1) 0.8 3.7 0.7 7.6 P Value 0.67 0.16 0.72 0.02 * A = BNL 3279_114; a = BNL 3279_112; B = CIR 316_202; and b = CIR 316_191 markers.

Charactersistics of Homozygous Stacked F3 Progeny (AABB) Compared to Parents Acala NemX Recombinant F3 Progenies LONREN Lines Fuzz Color White (fzg) Green (Fzglon) except ML (fzg) CIR 316 _202 _191 BNL 1231 _197 Type 1 _null _192 Type 2 BNL 3279 _112 _114 BNL 1066 _130 Type 1 _156 _139 Type 2 BNL 836 _207 _215 CONCLUSION: Crossovers in all 8 recombinants occurred between CIR 316 and BNL 1231.

Segregation of Backcross-1 Progeny from F2 Recombinant Plants Genotype* F2 Parent Plant† 3279 316 ME-25 ME-20 ML-24 ML-37 MO-33 (No. BC1 Plants in Genotype) Aa Bb 4 5 2 bb 6 - 8 aa 3 Chi Square (1:1) 0.4 0.5 3.6 P Value 0.53 0.48 0.06 * A = BNL 3279_114; a = BNL 3279_112; B = CIR 316_202; and b = CIR 316_191. † ME-25, ME-20, and ML-24 backcrossed to Deltapine 493; ML-37 and MO-30 backcrossed to Tamcot CAMD-E.

Segregation of BC2 Progeny from Doubly Heterozygous BC1 Progeny of Recombinant ME-25 Genotype* aa bb Aa bb aa Bb Aa Bb Observed 18 7 9 16 Expected 12.5 Chi Square (1:1:1:1) = 6.8 Recombinant % = 32 P Value = 0.08 * A = BNL 3279_114; a = BNL 3279_112; B = CIR 316_202; and b = CIR 316_191.

Fiber Quality of Selfed Progeny and Homozygous Sibs of BC7S1 Recombinant ME-25 Genotype* MIC UHM STR UI AABb (ME-25) 4.60 1.22 36.1 88.9 aaBB CIR 316_202 Sib 4.18 1.26 28.5 88.1 AAbb BNL 3279_114 Sib 3.84 1.25 31.6 87.8 AAbb BNL 3279_114 Progeny 4.16 33.4 88.3 AABB Stacked Progeny 4.44 1.27 32.3 88.5 aa BB Acala NemX (Type 2) 4.9 33.2 87.2 * A = BNL 3279_114; a = BNL 3279_112; B = CIR 316_202; and b = CIR 316_191. CONCLUSION: ME-25 Stacked has excellent fiber quality.

Fiber Quality of Selfed Progeny and Homozygous Sibs of BC7S1 Recombinant MO-30 Genotype* MIC UHM STR UI AABb (MO-30) 5.10 1.17 34.4 87.3 aaBB CIR 316_202 Sib 5.03 1.18 30.5 87.0 AAbb BNL 3279_114 Sib 3.86 1.21 29.6 AAbb BNL 3279_114 Progeny 4.06 1.23 29.2 86.6 AABB Stacked Progeny 4.75 1.25 30.1 86.7 aa BB Acala NemX (Type 1) 4.65 33.9 87.5 * A = BNL 3279_114; a = BNL 3279_112; B = CIR 316_202; and b = CIR 316_191. CONCLUSION: MO-30 Stacked has very good fiber quality.

Comparison of Fiber Quality of Stacked Lines with the White Fuzzed ML Line MIC UHM STR UI LONREN ML 4.31 1.06 30.5 86.1 ME-25 Stack 4.44 1.27 32.3 88.5 MO-30 Stack 4.75 1.25 30.1 86.7 CONCLUSION: Stacked white fuzz lines have fiber quality superior to that of LONREN ML Line.

Conclusions 1. Eight plants with a recombination between CIR 316_202 from Acala NemX and BNL 1232_null from four LONREN lines have been obtained along with more that 60 sib plants homozygous for either marker. 2. Homozygous recombinants from ME-25 (84 family) and MO-30 (122 family) have excellent fiber quality and will be further evaluated and probably released as new germplasm as early as the spring of 2008. 3. These recombinants and their sibs allow a critical evaluation of the proposed map location of the root-knot resistance genes rkn-1 and Mi2, and the reniform resistance gene Rrlon.

Acknowledgements Jose Quintana - Performing DNA marker analyses and preparing illustrations. Brandy Velten and Andrew Long - Performing DNA preparation and laboratory work. Dr. Forest A. Robinson - Determining the resistance of the LONREN parents used in these studies. Dr. Robert Nichols and Cotton Inc. - Providing financial assistance and fiber analyses.