Fluid membranes From biology to physics and back again

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Presentation transcript:

Fluid membranes From biology to physics and back again Introduction 1 Fluid membranes From biology to physics and back again TEM image Ana-Sunčana Smith smith@physik.fau.de Institut für Theoretische Physik I Universität Erlangen-Nürnberg, Germany

Statistical mechanics at reduced dimensionality Introduction 2 Statistical mechanics at reduced dimensionality self assembly – structural properties phase transitions (solid-gel-fluid) microfluidics mixtures - lateral diffusion, aggregation domain formation thermal fluctuations Soft matter interplay of the bending elasticity with the surface tension and adhesion chemical kinetics in confined geometry interplay between length and time scales Biomimetics understanding the relation between the physical properties of soft materials and their biological role Nano/Bio-technology bio-compatible materials and systems micro-manipulators and molecular probes bio-sensors 13.11.2018 Cluster of Excellence: Engineering of Advanced Materials

Chemical composition PtdCho – Phosphatidylcholine PtdEtn - Phosphatidylethanolamine PtdIns - Phosphatidylinositol PtdSer - Phosphatidylserine http://en.wikipedia.org/wiki/Membrane_lipids

Chemical composition Phosphoglycerides 13.11.2018

Chemical composition Sphingomyelin Phosphoglycerides 13.11.2018

Chemical composition Glycolipid- cerrebroside 13.11.2018 Cluster of Excellence: Engineering of Advanced Materials

Chemical composition 13.11.2018 Cluster of Excellence: Engineering of Advanced Materials

Chemical composition 13.11.2018 Cluster of Excellence: Engineering of Advanced Materials

Chemical composition Cholesterol Space association of SPHM and cholesterol 13.11.2018 Cluster of Excellence: Engineering of Advanced Materials

Self assembly

Self assembly Phase diagram for monoolein

Mixtures 13.11.2018

Self assembly Self assembly Degrees of freedom

Pipetting vesicles 13.11.2018

The cell membrane 13.11.2018

Cell membranes 13.11.2018

Cellm membranes 13.11.2018 Cluster of Excellence: Engineering of Advanced Materials

Principle curvatures 13.11.2018

Basic membrane shapes Shape C1 C2 Plane Cylinder + Sphere Saddle - Cylinder + Sphere Saddle - a | A membrane under a flat clathrin lattice. b | A membrane tube under a dynamin coat. c | The neck of a membrane bud. d | Pure clathrin cages assembled in vitro. (Heuser Lab for more fascinating images). Zimmerberg J and Kozlov MM (2005) Nature Reviews Molecular Cell Biology 6: 1

Spontaneous curvature Mechanisms by which proteins can generate membrane curvature. a | The scaffold mechanism. A rigid protein, or protein domain (for example, the BAR (Bin, amphiphysin, Rvs) domain), that has an intrinsic curvature binds to the membrane surface and bends the membrane beneath it. b | Polymerized coat proteins, which are sometimes linked to membranes through adaptor proteins (not shown), stabilize membrane curvature. c | The local spontaneous curvature mechanism is mediated by the insertion of amphipathic moieties of proteins between the polar headgroups of lipid molecules. 13.11.2018 Zimmerberg J and Kozlov MM (2005) Nature Reviews Molecular Cell Biology 6: 1

Reduced volume p1 < p2 Dp

Vesicle morphology The area difference model 13.11.2018

Area difference for the RBC 13.11.2018

Fluctuation amplitude

Measurement of membrane fluctuations Betz and Sykes Soft Matter, 2012,8, 5317-5326 13.11.2018