POST GRADUATE GOVERNMENT COLLEG SEC 11 CHANDIGARH )

STERILE INSECT RELEASE METhOD

Sterile insect The reproductive potential of insect is change The sterile insect achieved by physical or chemical agent The steril insect release method controlled the population of pest

METOD OF STERILITION PYSICAL STERILANT --- Insect steriled by exposure to ionizing radiation such as X rays and Gama rays And released in infested region Compete for mates with normal insects The pest population is reduced or wiped out PINK BOLLWORM completely EXAMPLE– When lepidoptera adult are treated with certain doses of radiation (5 to 12 krad), their F1 progeny will contain chromosomal translocations, which will cause varying .amounts of reproductive sterility in those F1 progeny. This picture demonstrates massive chromosomal translocations in an F1 male whose father received 6 krad of gamma radiation.

Radiation cause sterility Infecundity in females Sperm inactivation Inability to mate Dominant lethal mutation in reproductive cells of male or female

Chemosterilant Sterility produced in pests by chemical is called chemosterilant Chemical administered orally or by contact to a wide variety of insect It disrupt reproductive cycle at various stages The chemicals may disrupt the reproductive process of female and male insect respectively is called female chemosterilant and male chemosterilant

Type of chemosterilant Chemosterilant divided into tree group 1. Alkylation Agent -- Specially effective as a male sterilant Alkylation is a substitution of an alkyl group from a hydrogen atom (a) aziridined and non aziridines (b) sulphuric acid esters (c)nitrogen mustards 2. Antimetabolites– female sterilant (a) Analogues of purine ,pyrimictrines (b) Folic acid (c) Analogues of amino acids

CEMOSTERILANT CAUSE STERILITY (3) Miscellaneous compound (radiomimmetic cemical )---- These compounds contain radioactive isotopes of carbon , phosphorous, or other elements Methionine administered to such insects sterilized male (a) S– triazines – Hemel , Melamine (b) Posphoramide -- Hempa (c) Tin derivatives -- Triphenylltin chloride (d) other miscellaneous agent -- Colchicine , Coumarin CEMOSTERILANT CAUSE STERILITY Preventing the development of sperms or ova Cause death of ova after these have been produced Injure genetic make up of the sperm or ova tat fertilizd

Mode of application OF CEmosterilant (1) Oral Injection-: Natural food could also sprayed with cemosterilant Treated food is placed in infested area for insect to eat Example– housefly population in a poultry house could reduced sharply by applying corn meal bait containing 0.5% metapa to poultry dropping (2) Topical or contact (a) Cemosterilants only 2. Insecticide cemosterilant combination (b) Direct contact wit residues © Sterile male release method (3) Injection--- Injection is best procedure for quantitative evaluation of activity

ADVANTASES OF CEMOSTERILANT (1)Reproductive potential of population is reduced of extent sterility is achieved in natural population (2) bonus effect – If sexual competitiveness of an insect is maintained Sterile organisms will not be incapable of reproducing but reproductive capacity of normal unsterilized population (3) Space effect– Sterilized individual from treated environment can move into untreated areas And Effect reproductive potential of unexposed fertile individual (4) Population crush –Sterilized individuals will complete wit unsterilized individual for food and shelter in environment during their normal serving period

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