HS 4160 Critical Scientific Analysis Week 4: Utility of peripheral blood dual color, double fusion fluorescent in situ hybridization for BCR/ABL fusion to assess cytogenetic remission status in chronic myeloid leukemia HS 4160 Critical Scientific Analysis

According to the authors, D-FISH stands for: De-stained FISH Dual color FISH Dual color, double fusion FISH Tri color, double fusion FISH

One limitation of D-FISH is failure to detect cryptic translocation True False

D-FISH could Significantly increase false positive events Significantly decrease false negative events Significantly decrease sensitivity Significantly decrease specificity

The only exclusion criteria the authors used was: Prior history of other cancers Prior history of AML Prior history of any CML-directed therapy Prior history of conventional cytogenetic testing

Which of the following correctly describe the probe set used? ABL gene labelled with green BCR gene on 9q34 This is a home-made probe set Fusion indicates ABL/BCR translocation

Which software program the authors used for their statistical analysis? SPSS SAS Excel GraphPad

From table I, which triplicate collection showed best correlation?

The outlier from Figure 1 had: no BCR/ABL fusion in bone marrow but with t(9;22) by CC no t(9;22) by CC but high rate of BCR/ABL fusion in bone marrow no t(9;22) by CC but high rate of BCR/ABL fusion in blood no BCR/ABL fusion in blood but with t(9;22) by CC

From table II, which pair has more samples? Bone marrow D-FISH vs. CC Peripheral blood D-FISH vs. CC Peripheral blood D-FISH vs. Bone marrow D-FISH