GAL 3 GALACTOSE + ATP GAL 3 GALACTOSE ATP GAL 80 GAL 4

Specific induction factors are, exclusively and typically in fungi, Zn binuclear cluster proteins.

A/SC-2-C-6-C-4/7-P-0/1-C-2-C-4/7-C

UaY: CGG-6x-CCG PPR1: CGG-6x-CCG GAL4: CGG-11x-CCG PUT3: CGG-10x-CCG

5’ CGG-X-CCG Inverted GCC-X-GGC 5’ 5’ CGG-X-CGG Direct GCC-X-GCC 5’ 5’ CCG-X-CGG Everted GGC-X-GCC 5’

Protein: CreA Zn finger competitor wt prnd22 prnd22 wt wt probe prnd22 probe

+ Treatment Denature

FP C

Protein: CreA Zn finger competitor wt prnd22 prnd22 wt wt probe prnd22 probe

+ RETARDATION GEL FP C

+ FP C RETARDATION GEL FP C

FP C

+ FP C RETARDATION GEL C

FP C

Treatment (DMS) DNA extraction Piperidine Specificprimer extension Ligation (non-specific linker) PCR Thus, generation of molecules within the region of interest, all ending at a methylation site Primer extension Thus, generation of molecules, terminally labelled on a single strand, all starting at the same point, all ending at methylation site, continuation as for any foot print.

Non-induced Induced GAL1 GAL10 UAS PHO5