Effects of Scaffold Material Used in Cardiovascular Surgery on Mesenchymal Stem Cells and Cardiac Progenitor Cells Chani Hodonsky, MPH, BS, Lakshmi Mundada,

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Presentation transcript:

Effects of Scaffold Material Used in Cardiovascular Surgery on Mesenchymal Stem Cells and Cardiac Progenitor Cells Chani Hodonsky, MPH, BS, Lakshmi Mundada, MS, Shuyun Wang, MD, PhD, Russell Witt, MD, MS, Gary Raff, MD, Sunjay Kaushal, MD, PhD, Ming-Sing Si, MD The Annals of Thoracic Surgery Volume 99, Issue 2, Pages 605-611 (February 2015) DOI: 10.1016/j.athoracsur.2014.08.071 Copyright © 2015 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 Cell seeding of pSIS. Heterogeneous aggregation of human mesenchymal stem cells (A) and cardiac progenitor cells (B) on porcine small intestinal submucosa under bright field microscopy (×10 magnification). Cell aggregation indicated by white arrows. The Annals of Thoracic Surgery 2015 99, 605-611DOI: (10.1016/j.athoracsur.2014.08.071) Copyright © 2015 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 Effects of scaffold material on mesenchymal stem cells (MSC) and cardiac progenitor cells proliferation (CPC). Polytetrafluoroethylene (PTFE) and porcine small intestinal submucosa (pSIS) had different effects on MSC and CPC proliferation. Porcine SIS significantly promoted both MSC and CPC proliferation as compared with PTFE. The Annals of Thoracic Surgery 2015 99, 605-611DOI: (10.1016/j.athoracsur.2014.08.071) Copyright © 2015 The Society of Thoracic Surgeons Terms and Conditions

Fig 3 The RNA expression of mesenchymal stem cells (MSCs) and cardiac progenitor cells (CPCs) seeded on porcine small intestinal submucosa (pSIS). Relative quantitative expression of (A) MYC, (B) MEF2C, (C) GATA4, (D) SLIT3, (E) PDGF, (F) VEGFA, (G) FLT1, and (H) ACTC1 was analyzed 7 days post seeding on pSIS. (Black bars represent control CPCs; grey, CPCs on pSIS; black stripes, control MSCs; and grey stripes, MSCs on pSIS; in a t test of differential expression, *p < 0.01.) The Annals of Thoracic Surgery 2015 99, 605-611DOI: (10.1016/j.athoracsur.2014.08.071) Copyright © 2015 The Society of Thoracic Surgeons Terms and Conditions

Fig 4 Cardiac progenitor cells (CPC) and mesenchymal stem cells (MSC) vascular endothelial growth factor A (VEGFA) production is increased by scaffolds. The MSC VEGFA supernatant levels were increased by porcine small intestinal submucosa (pSIS) but not polytetrafluoroethylene (PTFE). The CPC VEGFA supernatant levels were significantly increased by both pSIS and PTFE. The Annals of Thoracic Surgery 2015 99, 605-611DOI: (10.1016/j.athoracsur.2014.08.071) Copyright © 2015 The Society of Thoracic Surgeons Terms and Conditions