Tracy I. George, Joanna E. Wrede, Charles D. Bangs, Athena M

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Low-Grade B-Cell Lymphomas With Plasmacytic Differentiation Lack PAX5 Gene Rearrangements Tracy I. George, Joanna E. Wrede, Charles D. Bangs, Athena M. Cherry, Roger A. Warnke, Daniel A. Arber The Journal of Molecular Diagnostics Volume 7, Issue 3, Pages 346-351 (August 2005) DOI: 10.1016/S1525-1578(10)60563-6 Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 1 PAX-5 FISH probe design. BACs RP11-12P15 and RP11-243F8 (labeled green) span the PAX5 gene and ∼250-kb telomeric (3′) to the PAX5 gene. RP11-501A2 (labeled red) localizes to a region centromeric (5′) to previously described breakpoints. Arrows indicate sequenced PAX-5 translocations described in the literature.456810111213 Base position identified using the UCSC Genome Browser, http://genome.ucsc.edu. The Journal of Molecular Diagnostics 2005 7, 346-351DOI: (10.1016/S1525-1578(10)60563-6) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 2 Co-hybridization of KIS-1 metaphase spread with the dual-color CBFB probe specific for band 16q22 and differentially labeled whole chromosome paint probes for chromosomes 9 and 14 show two chromosomes consisting primarily of chromosome 9 material (labeled red) and representing a normal 9 homolog and a derivative 9 consistent with a translocation t(9;14). Two additional rearranged chromosomes are identified, a derivative chromosome 14 (labeled green) with a small distal chromosome 9 segment (labeled red), and a complex derivative chromosome 16 [der(16)] identified by the CBFB probe and containing distal juxtaposed red chromosome 9 and green chromosome 14 segments. The Journal of Molecular Diagnostics 2005 7, 346-351DOI: (10.1016/S1525-1578(10)60563-6) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 3 KIS-1 metaphase (A) and interphase nuclei (B) hybridized with PAX5 probe. Red signals correspond to the centromeric (5′) end of the PAX5 probe on chromosome 9p13, whereas green signals indicate the telomeric (3′) portion of the probe. A fusion signal indicates an intact PAX5 gene. Separate red and green signals (split signal) indicate a PAX5 rearrangement. The Journal of Molecular Diagnostics 2005 7, 346-351DOI: (10.1016/S1525-1578(10)60563-6) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 4 Representative interphase nuclei from case 27 hybridized with PAX5 probe. Two fusion signals indicate intact PAX5 genes with no translocation. The Journal of Molecular Diagnostics 2005 7, 346-351DOI: (10.1016/S1525-1578(10)60563-6) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

Figure 5 Representative interphase nuclei from case 22 hybridized with PAX5 probe. Four fusion signals indicate duplication of chromosome 9, confirmed by FISH with telomeric 9p and 9q probes (data not shown). The Journal of Molecular Diagnostics 2005 7, 346-351DOI: (10.1016/S1525-1578(10)60563-6) Copyright © 2005 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions