Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem.

Slides:

Advertisements

Similar presentations

Overexpression of endoplasmic reticulum-resident chaperone attenuates cardiomyocyte death induced by proteasome inhibition by Hai Ying Fu, Tetsuo Minamino,

Advertisements

Cell Physiol Biochem 2017;42:2582– DOI: /

Comparative Profiling of Triple-Negative Breast Carcinomas Tissue Glycoproteome by Sequential Purification of Glycoproteins and Stable Isotope Labeling.

TNF-α- Mediated-p38-Dependent Signaling Pathway Contributes to Myocyte Apoptosis in Rats Subjected to Surgical Trauma Cell Physiol Biochem 2015;35:

Leukotriene B4 Inhibits L-Type Calcium Channels via p38 Signaling Pathway in Vascular Smooth Muscle Cells Cell Physiol Biochem 2015;37:

Cell Physiol Biochem 2014;33: DOI: /

Inducible EGFR T790M-Mediated Gefitinib Resistance in Non-small Cell Lung Cancer Cells Does Not Modulate Sensitivity to PI103 Provoked Autophagy Flavia.

Nasal Cavity Paraganglioma: Literature Review and Discussion of a Rare Case Biomed Hub 2017;2: DOI: / © 2017 The Author(s) Published.

Case Rep Oncol 2017;10:361– DOI: /

OPN -443C>T Genetic Polymorphism and Tumor OPN Expression are Associated with the Risk and Clinical Features of Papillary Thyroid Cancer in a Chinese Cohort.

Cell Physiol Biochem 2013;32: DOI: /

Cell Physiol Biochem 2016;38: DOI: /

Astragaloside IV Enhances Cisplatin Chemosensitivity in Non-Small Cell Lung Cancer Cells Through Inhibition of B7-H3 Cell Physiol Biochem 2016;40:

Extracellular HSP70 Activates ERK1/2, NF-kB and Pro-Inflammatory Gene Transcription Through Binding with RAGE in A549 Human Lung Cancer Cells Cell Physiol.

A Novel Cinnamide YLT26 Induces Breast Cancer Cells Apoptosis via ROS-Mitochondrial Apoptotic Pathway in Vitro and Inhibits.

Effect of Shikonin on Spinal Cord Injury in Rats Via Regulation of HMGB1/TLR4/NF-kB Signaling Pathway Cell Physiol Biochem 2017;43:481–491 - DOI: /

AntimiR-30b Inhibits TNF-α Mediated Apoptosis and Attenuated Cartilage Degradation through Enhancing Autophagy Cell Physiol Biochem 2016;40:

Effects of Glycyrrhizin in a Mouse Model of Lung Adenocarcinoma

Cell Physiol Biochem 2015;37: DOI: /

Cell Physiol Biochem 2016;38: DOI: /

Up-Regulation of TGF-β Promotes Tendon-to-Bone Healing after Anterior Cruciate Ligament Reconstruction using Bone Marrow-Derived Mesenchymal Stem Cells.

Cantharidin Inhibits the Growth of Triple-Negative Breast Cancer Cells by Suppressing Autophagy and Inducing Apoptosis in Vitro and in.

Downregulation of MicroRNA-145 Caused by Hepatitis B Virus X Protein Promotes Expression of CUL5 and Contributes to Pathogenesis of Hepatitis B Virus-Associated.

Trends in Gene Expression Profiling for Prostate Cancer Risk Assessment: A Systematic Review Biomed Hub 2017;2: DOI: / © 2017 The.

Bufalin Inhibits the Differentiation and Proliferation of Cancer Stem Cells Derived from Primary Osteosarcoma Cells through Mir-148a Cell Physiol Biochem.

Case Rep Gastroenterol 2016;10:338– DOI: /

Potential Molecular Mechanisms for Improved Prognosis and Outcome with Neoadjuvant Chemotherapy Prior to Laparoscopical Radical Hysterectomy for Patients.

Cell Physiol Biochem 2017;41:921– DOI: /

MicroRNA-101 Inhibits Growth, Proliferation and Migration and Induces Apoptosis of Breast Cancer Cells by Targeting Sex-Determining Region Y-Box 2 Cell.

Cell Physiol Biochem 2013;31: DOI: /

Plasma Cell Leukemia Presenting as a Chest Wall Mass: A Case Report

Pancreatic Acinar Cell Carcinoma

Nm23-H1 Regulates Glucose-Stimulated Insulin Secretion in Pancreatic β-Cells via Arf6-Rac1 Signaling Axis Cell Physiol Biochem 2013;32: DOI: /

Effect of microRNA-135a on Cell Proliferation, Migration, Invasion, Apoptosis and Tumor Angiogenesis Through the IGF-1/PI3K/Akt Signaling Pathway in Non-Small.

Baicalein, a Natural Anti-Cancer Compound, Alters MicroRNA Expression Profiles in Bel-7402 Human Hepatocellular Carcinoma Cells Cell Physiol Biochem 2017;41:1519–1531.

The Long Non-Coding RNA XIST Interacted with MiR-124 to Modulate Bladder Cancer Growth, Invasion and Migration by Targeting Androgen Receptor (AR) Cell.

Verrucous Squamous Cell Cancer in the Esophagus: An Obscure Diagnosis

Elevated Apoptosis in the Liver of Dairy Cows with Ketosis

Cell Physiol Biochem 2017;44:1867– DOI: /

Epigallocatechin-3-Gallate Inhibits Matrix Metalloproteinase-9 and Monocyte Chemotactic Protein-1 Expression Through the 67-κDa Laminin Receptor and the.

Elevated FOXC2 Expression Promotes Invasion of HCC Cell Lines and is Associated with Poor Prognosis in Hepatocellular Carcinoma Cell Physiol Biochem 2017;44:99–109.

Mechanical Loading Improves Tendon-Bone Healing in a Rabbit Anterior Cruciate Ligament Reconstruction Model by Promoting Proliferation and Matrix Formation.

Cell Physiol Biochem 2015;36: DOI: /

ZNF750 Expression Is a Potential Prognostic Biomarker in Esophageal Squamous Cell Carcinoma Oncology - DOI: / Fig. 1. The immunohistochemical.

Epidermal Growth Factor Promotes Proliferation and Migration of Follicular Outer Root Sheath Cells via Wnt/β-Catenin Signaling Cell Physiol Biochem 2016;39:

Influence of Four Radiotracers in PET/CT on Diagnostic Accuracy for Prostate Cancer: A Bivariate Random-Effects Meta-Analysis Cell Physiol Biochem 2016;39:

Cell Physiol Biochem 2016;38: DOI: /

Snail Enhances Glycolysis in the Epithelial-Mesenchymal Transition Process by Targeting FBP1 in Gastric Cancer Cell Physiol Biochem 2017;43:31–38 - DOI: /

Urinary Trypsin Inhibitor Attenuates Acute Lung Injury by Improving Endothelial Progenitor Cells Functions Cell Physiol Biochem 2015;36: DOI: /

The Chemopreventive Effect of Tanacetum Polycephalum Against LA7-Induced Breast Cancer in Rats and the Apoptotic Effect of a Cytotoxic Sesquiterpene.

Chronic Hepatitis B Infection is Associated with Decreased Risk of Preeclampsia: A Meta-Analysis of Observational Studies Cell Physiol Biochem 2016;38:

Cerebral Mast Cells Participate In Postoperative Cognitive Dysfunction by Promoting Astrocyte Activation Cell Physiol Biochem 2016;40: DOI: /

Lgr5-Positive Cells are Cancer-Stem-Cell-Like Cells in Gastric Cancer

Cardioprotective Effects of Combined Therapy with Hyperbaric Oxygen and Diltiazem Pretreatment on Myocardial Ischemia-Reperfusion Injury in Rats Cell Physiol.

Cell Physiol Biochem 2016;39: DOI: /

Ling Yang, Ping Li, Suneng Fu, Ediz S. Calay, Gökhan S. Hotamisligil

Dienogest enhances autophagy induction in endometriotic cells by impairing activation of AKT, ERK1/2, and mTOR JongYeob Choi, Ph.D., MinWha Jo, M.S.,

Inhibition of Notch Signaling Promotes the Adipogenic Differentiation of Mesenchymal Stem Cells Through Autophagy Activation and PTEN-PI3K/AKT/mTOR Pathway.

Selective interference of mTORC1/RAPTOR protects against human disc cellular apoptosis, senescence, and extracellular matrix catabolism with Akt and autophagy.

Inducible EGFR T790M-Mediated Gefitinib Resistance in Non-small Cell Lung Cancer Cells Does Not Modulate Sensitivity to PI103 Provoked Autophagy Flavia.

Thy-1/β3 Integrin Interaction-Induced Apoptosis of Dermal Fibroblasts Is Mediated by Up-Regulation of FasL Expression Manuela Schmidt, Danny Gutknecht,

Inhibition of mammalian target of rapamycin: Two goals with one shot?

Leptin promotes apoptosis and inhibits autophagy of chondrocytes through upregulating lysyl oxidase-like 3 during osteoarthritis pathogenesis Z.M. Huang,

S. D'Adamo, O. Alvarez-Garcia, Y. Muramatsu, F. Flamigni, M.K. Lotz

Figure 2 Autophagosome formation in mammalian cells

Triptolide Restores Autophagy to Alleviate Diabetic Renal Fibrosis through the miR p/PTEN/Akt/mTOR Pathway Xiao-yu Li, Shan-shan Wang, Zhe Han,

Oncogenic Human Papillomavirus: Application of CRISPR/Cas9 Therapeutic Strategies for Cervical Cancer Cell Physiol Biochem 2017;44:2455– DOI: /

Digital Medicine: A Primer on Measurement

Hyperplastic alveolar epithelial type II cells show severe endoplasmic reticulum stress and consecutive apoptosis. Hyperplastic alveolar epithelial type.

Fig. 5 C9orf72 knockdown disrupts autophagy induction.

Presentation transcript:

Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem 2017;44:133–151 - DOI:10.1159/000484622 Fig. 1. mRNA and protein levels of CHOP, GRP78, IRE1α, PI3K, AKT and mTOR in the mutant and wild-type p53 groups. Note: A. Diagram of protein expression levels of CHOP, GRP78, IRE1α, PI3K, AKT and mTOR detected by Western blotting; B. Relative protein expression levels of CHOP, GRP78, IRE1α, PI3K, AKT and mTOR in the mutant and wild-type p53 groups; C. Relative mRNA expression levels of CHOP, GRP78, IRE1α, PI3K, AKT and mTOR in the mutant and wild-type p53 groups; *P < 0.05, indicates a comparison with P53-wild-type group; CHOP, C/EBP homologous protein; GRP78, glucose-regulated protein 78; IRE1α, inositolrequiring enzyme-1α; PI3K, phosphatidyl inositol 3-kinase; Akt, protein kinase B; mTOR, mammalian target of rapamycin. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem 2017;44:133–151 - DOI:10.1159/000484622 Fig. 2. mRNA and protein expression levels of CHOP, GRP78 and IRE1α in mutant p53 LC cells in each group Note: A. Protein levels of CHOP, GRP78 and IRE1α in mutant p53 LC cells detected by Western blotting; B. Protein levels of CHOP, GRP78 and IRE1α in mutant p53 H322 cells; C. mRNA expression levels of CHOP, GRP78 and IRE1α in mutant p53 H322 cells; D. Protein levels of CHOP, GRP78 and IRE1α in mutant p53 NCI-H157 cells; E. mRNA expression levels of CHOP, GRP78 and IRE1α in mutant p53 NCI-H157 cells; *P<0.05, represents a comparison with the blank group; #P<0.05, represents a comparison with the ERS group; LC, lung cancer; ERS, endoplasmic reticulum stress; CHOP, C/EBP homologous protein; GRP78, glucose-regulated protein 78; IRE1α, inositol-requiring enzyme-1α. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem 2017;44:133–151 - DOI:10.1159/000484622 Fig. 3. mRNA and protein expression levels of PI3K, AKT and mTOR in mutant p53 LC cells in each group. Note: A. Protein levels of PI3K, AKT and mTOR in H322 and NCI-H157 cell lines detected by Western blotting; B. Relative protein expression levels of PI3K, AKT and mTOR in the mutant p53 H322 cell line; C. Relative mRNA expression levels of PI3K, AKT and mTOR in the mutant p53 H322 cell line; D. Relative protein expression of PI3K, AKT and mTOR in the mutant p53 NCI-H157 cell line; E. Relative mRNA expression of PI3K, AKT and mTOR in the mutant p53 NCI-H157 cell line; *P<0.05, represents a comparison with the blank group; #P<0.05, represents a comparison with the ERS group; LC, lung cancer; ERS, endoplasmic reticulum stress; PI3K, phosphatidyl inositol 3-kinase; Akt, protein kinase B; mTOR, mammalian target of rapamycin © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem 2017;44:133–151 - DOI:10.1159/000484622 Fig. 4. mRNA and protein expression levels of the autophagy factors LC3-II/LC3-I, Atg5, and Atg7 in mutant p53 LC cells in each group Note: A. Protein levels of LC3-I, LC3-II, Atg5, and Atg7 in the H322 and NCI-H157 cell lines for the five groups detected by Western blotting; B. Relative protein expression levels of LC3-II/LC3-I, Atg5 and Atg7 in the mutant p53 H322 cell line for the five groups; C. Relative mRNA expression levels of LC3-II/LC3-I, Atg5, and Atg7 in the mutant p53 H322 cell line; D. Relative protein expression levels of LC3-II/LC3-I, Atg5, and Atg7 in the mutant p53 NCI-H157 cell line; E. Relative mRNA expression levels of LC3-II/LC3-I, Atg5, and Atg7 in the mutant p53 NCI-H157 cell line; *P<0.05, represents a comparison with the blank group; #P<0.05. represents a comparison with the ERS group; &P<0.05, represents a comparison with the rapamycin group; LC, lung cancer; ERS, endoplasmic reticulum stress; LC3, light chain 3; Atg5, autophagyrelated gene 5; Atg7, autophagy-related gene 7. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem 2017;44:133–151 - DOI:10.1159/000484622 Fig. 5. Number of autophagosomes formed in mutant p53 LC cells in each group Note: Blue represents the nucleus; Green represents LC3 fusion protein-marked autophagosomes; Scale bar = 10 µm; Red arrows indicate autophagosomes; *P<0.05, represents a comparison with the blank group; #P<0.05, represents a comparison with the ERS group; &P<0.05, represents a comparison with the rapamycin group; LC, lung cancer; ERS, endoplasmic reticulum stress. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem 2017;44:133–151 - DOI:10.1159/000484622 Fig. 6. mRNA and protein levels of caspase-3 and caspase-12 in mutant p53 LC cells in each groupNote: A. Protein levels of caspase-3, caspase-12, cleaved caspase-3 and cleaved caspase-12 in the H322 and NCI-H157 cell lines for the five groups detected by Western blotting; B. Relative protein expression levels of caspase-3, caspase-12, cleaved caspase-3 and cleaved caspase-12 in the mutant p53 H322 cell line; C. Relative mRNA expression levels of caspase-3 and caspase-12 in the mutant p53 H322 cell line; D. Relative protein expression levels of caspase-3, caspase-12, cleaved caspase-3 and cleaved caspase-12 in the mutant p53 NCI-H157 cell line; E. Relative mRNA expression levels of caspase-3 and caspase-12 in the mutant p53 NCI-H157 cell line; *P<0.05, represents a comparison with the blank group; #P<0.05, represents a comparison with the ERS group; &P<0.05, represents a comparison with the rapamycin group; LC, lung cancer; ERS, endoplasmic reticulum stress © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem 2017;44:133–151 - DOI:10.1159/000484622 Fig. 7. Apoptosis rates in mutant p53 LC cells induced by ERS in each groupNote: A. Cell apoptosis in the mutant p53 H322 cell line for the five groups detected by flow cytometry; B. Cell apoptosis rate in the mutant p53 NCI-H157 cell line for the five groups; * P < 0 . 0 5 , represents a comparison with the blank group; #P<0.05, represents a comparison with the ERS group; & represents a comparison with the rapamycin group; LC, lung cancer; ERS, endoplasmic reticulum stress. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0

Endoplasmic Reticulum Stress Promotes Autophagy and Apoptosis and Reduces Chemotherapy Resistance in Mutant p53 Lung Cancer Cells Cell Physiol Biochem 2017;44:133–151 - DOI:10.1159/000484622 Fig. 8. Proliferation in mutant p53 LC cells induced by ERS in each groupNote: A. Cell proliferation in the mutant p53 H322 cell line detected by BrdU cell proliferation assay; Scale bar = 20 um; B. Cell proliferation in the mutant p53 NCI-H157 cell line detected by BrdU cell proliferation assay; C: Statistical analysis of BrdU-positive H1322 cells; D: Statistical analysis of BrdU-positive NCI-H157 cells; *P<0.05, represents a comparison with the blank group; #P<0.05, represents a comparison with the ERS group; BrdU, 5’bromo-2’deoxyuridine; LC, lung cancer; ERS, endoplasmic reticulum stress. © 2017 The Author(s). Published by S. Karger AG, Basel - CC BY-NC-ND 4.0