Cord blood mononuclear cells and milk-specific T-cell clones are tools to evaluate the residual immunogenicity of hydrolyzed milk formulas Zsolt Szépfalusi,

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Cord blood mononuclear cells and milk-specific T-cell clones are tools to evaluate the residual immunogenicity of hydrolyzed milk formulas Zsolt Szépfalusi, MDa, Ivo Nentwich, MDa, Eva Josta, Marianne Gerstmayra, Christof Ebner, MDb, Thomas Frischer, MDa, Radvan Urbanek, MDa Journal of Allergy and Clinical Immunology Volume 101, Issue 4, Pages 514-520 (April 1998) DOI: 10.1016/S0091-6749(98)70359-8 Copyright © 1998 Mosby, Inc. Terms and Conditions

FIG. 1. Cellular proliferative response of CBMCs to HFs FIG.1. Cellular proliferative response of CBMCs to HFs. CBMCs from 71 term newborns were incubated with 50 μg/ml HF, and mean of proliferation index (+SEM) was determined after 6 days of culture in triplicates. Milk proteins and HFs: whey proteins ( A: α-lactalbumin, B: β-lactoglobulin, and C: bovine serum albumin), casein (D: α-casein, E: β-casein, and F: κ-casein), adapted cow's milk (G: Aptamil 1), partial whey-based HFs (H: Beba HA and I: Nidina HA), extensive whey-based HFs (J: Alfaré, K: Prophilac, L: Hipp HA, M: Peptamine Junior, and N: MOH), partial whey/casein-based HF (O: Aptamil hypoallergen), extensive whey/casein-based HF (P: Damira), partial casein-based peptide component (Q: CAH), and extensive casein-based HF (R: Nutramigen). Background level is defined at stimulation index of 1. Generalized linear model was used for comparison of groups of antigens. Probability values are for comparison between groups (p < 0.0001). Journal of Allergy and Clinical Immunology 1998 101, 514-520DOI: (10.1016/S0091-6749(98)70359-8) Copyright © 1998 Mosby, Inc. Terms and Conditions

FIG. 2. β-Casein–specific TCCs proliferate in response to pHF and eHF FIG.2. β-Casein–specific TCCs proliferate in response to pHF and eHF. Twenty-one β-casein–specific TCCs were stimulated with milk proteins and hydrolyzed milk formula. Percent proliferating TCCs is expressed. Milk proteins and HFs: whey proteins (A, B, and C), casein (D, E, and F), adapted cow's milk (G), partial whey-based HFs (H and I), extensive whey-based HFs (J, K, L, M, and N), partial whey/casein-based HF (O), extensive whey/casein-based HF (P), partial casein-based peptide component (Q), and extensive casein-based HF (R). S represents positive control on stimulation of TCCs with mitogen phytohemagglutinin at 10 mg/ml. Journal of Allergy and Clinical Immunology 1998 101, 514-520DOI: (10.1016/S0091-6749(98)70359-8) Copyright © 1998 Mosby, Inc. Terms and Conditions

FIG.3. β-Casein–specific TCCs proliferate and produce cytokines in response to pHFs and eHFs. TCCs (10 5) were incubated with antigen (50 μg/ml) for 48 hours, and proliferation was assessed by tritiated thymidine incorporation for 16 hours. Soluble cytokines (IFN-γ and IL-4) were measured in supernatants after 48 hours by specific ELISA. Results from three representative TCCs (bC15, bC21, and bC71) are shown. All figures representing proliferation, IFN-γ production, and IL-4 production, respectively, are shown in same scale. Proliferation is expressed as counts per minute (cpm). Cytokines are expressed in picograms per milliliter. Milk proteins and HFs: whey proteins (A, B, and C), casein (D, E, and F), adapted cow's milk (G), partial whey-based HFs (H and I), extensive whey-based HFs (J, K, L, M, and N), partial whey/casein-based HF (O), extensive whey/casein-based HF (P), partial casein-based peptide component (Q), and extensive casein-based HF (R). Journal of Allergy and Clinical Immunology 1998 101, 514-520DOI: (10.1016/S0091-6749(98)70359-8) Copyright © 1998 Mosby, Inc. Terms and Conditions