Expression of Endomucin, a Novel Endothelial Sialomucin, in Normal and Diseased Human Skin Annegret Kuhn, MD, Gertrud Brachtendorf, Frank Kurth, Monika.

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Expression of Endomucin, a Novel Endothelial Sialomucin, in Normal and Diseased Human Skin Annegret Kuhn, MD, Gertrud Brachtendorf, Frank Kurth, Monika Sonntag, Ulrike Samulowitz, Dieter Metze, Dietmar Vestweber Journal of Investigative Dermatology Volume 119, Issue 6, Pages 1388-1393 (December 2002) DOI: 10.1046/j.1523-1747.2002.19647.x Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 1 Expression of endomucin on vascular endothelium in normal human skin. Paraffin sections of tongue (a–c), scalp (d–f), actinic skin (g–i), palmar skin (k–m), and genital mucous membrane (n–p) were analyzed by immunohistochemical methods using the MoAb L6H10 against endomucin (a,d,g, k,n) and, for control purposes, the polyclonal rabbit antibody against von Willebrand factor (b,e,h,l,o) and no first antibody (c,f,i,m,p). Scale bar=50 μm. Journal of Investigative Dermatology 2002 119, 1388-1393DOI: (10.1046/j.1523-1747.2002.19647.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 2 Expression of endomucin in specimens of normal human skin by in situ hybridization. A specific endothelial signal for endomucin messenger RNA was detected by in situ hybridization in the dermis of normal human skin using an anti-sense probe (a) in contrast to negative control using a sense probe (b). Scale bar=50 μm. Journal of Investigative Dermatology 2002 119, 1388-1393DOI: (10.1046/j.1523-1747.2002.19647.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 3 Western blot analysis of endomucin in human skin. Endomucin was immunoprecipitated with a polyclonal antibody against endomucin from human umbilical vein endothelial cell lysates (lanes a and c) and from lysates from normal human skin (lanes b and d). Using the MoAb L6H10 for western blot analysis, a specific signal for endomucin was detected (lanes a and b), with a molecular mass of about 95 kDa. The mouse MoAb V7C7.1 was used as a negative control, and a specific signal was detected in neither human umbilical vein endothelial cells (lane c) nor human skin (lane d). Molecular mass markers (in kDa) are indicated on the left. Journal of Investigative Dermatology 2002 119, 1388-1393DOI: (10.1046/j.1523-1747.2002.19647.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 4 Expression pattern of endomucin in skin lesions from patients with different inflammatory diseases. Paraffin sections of atopic dermatitis (a–c), psoriasis (d–f), cutaneous lupus erythematosus (g–i), lichen planus (k–m), and T cell lymphoma (n–p) were analyzed by immunohistochemical methods using the MoAb L6H10 against endomucin (a,d,g,k,n) and, for control purposes, the polyclonal rabbit antibody against von Willebrand factor (b,e,h,l,o) and no first antibody (c,f,i,m,p). Scale bar=50 μm. Journal of Investigative Dermatology 2002 119, 1388-1393DOI: (10.1046/j.1523-1747.2002.19647.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions

Figure 5 Expression pattern of endomucin in skin lesions from patients with benign and malignant vascular tumors. Paraffin sections of hemangioma (a–c), pyogenic granuloma (d–f), Kaposi's sarcoma (g–i), angiolipoma (k–m), and angiosarcoma (n–p) were analyzed by immunohistochemical methods using the MoAb L6H10 against endomucin (a,d,g,k,n) and, for control purposes, the polyclonal rabbit antibody against von Willebrand factor (b,e,h,l,o) and no first antibody (c,f,i,m,p). Scale bar=50 μm. Journal of Investigative Dermatology 2002 119, 1388-1393DOI: (10.1046/j.1523-1747.2002.19647.x) Copyright © 2002 The Society for Investigative Dermatology, Inc Terms and Conditions