Rubella persistence in epidermal keratinocytes and granuloma M2 macrophages in patients with primary immunodeficiencies Ludmila Perelygina, PhD, Stanley.

Slides:

Advertisements

Similar presentations

Control of immunopathology during chikungunya virus infection Caroline Petitdemange, PhD, Nadia Wauquier, PhD, Vincent Vieillard, PhD Journal of Allergy.

Advertisements

Douglas A. Plager, PhD, Ellen A. Weiss, Gail M. Kephart, BS, Robert M

Inhaled long-acting β2 agonists enhance glucocorticoid receptor nuclear translocation and efficacy in sputum macrophages in COPD Rubaiyat Haque, MBBS,

Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion

Loss of sirtuin 1 (SIRT1) disrupts skin barrier integrity and sensitizes mice to epicutaneous allergen challenge Mei Ming, PhD, Baozhong Zhao, PhD, Christopher.

Live rubella virus vaccine long-term persistence as an antigenic trigger of cutaneous granulomas in patients with primary immunodeficiency C. Bodemer,

Douglas A. Plager, PhD, Ellen A. Weiss, Gail M. Kephart, BS, Robert M

Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.

Role of fibroblasts in the pathogenesis of atopic dermatitis

Evidence for intranasal antinuclear autoantibodies in patients with chronic rhinosinusitis with nasal polyps Bruce K. Tan, MD, Quan-Zhen Li, PhD, Lydia.

Intravenous immunoglobulin treatment abrogates transplacental autoantibody transfer in a murine pemphigus model Sachiko Ono, MD, PhD, Gyohei Egawa, MD,

Complete human CD1a deficiency on Langerhans cells due to a rare point mutation in the coding sequence Daniela Cerny, PhD, Duyen Huynh Thi Le, MSc, Trung.

IL-25 as a novel therapeutic target in nasal polyps of patients with chronic rhinosinusitis Hyun-Woo Shin, MD, PhD, Dong-Kyu Kim, MD, Min-Hyun Park, MD,

Frequent Detection of Herpes Simplex Virus Antigen in Skin and Peripheral Blood CD34+ Mononuclear Cells from Patients with Graft-versus-Host Disease

Increased TGF-β2 in severe asthma with eosinophilia

Dual nature of T cell–epithelium interaction in chronic rhinosinusitis

Alefacept (lymphocyte function-associated molecule 3/IgG fusion protein) treatment for atopic eczema Dagmar Simon, MD, Jennifer Wittwer, MD, Ganna Kostylina,

Eosinophil extracellular DNA traps in skin diseases

Topical application of a vitamin D3 analogue and corticosteroid to psoriasis plaques decreases skin infiltration of TH17 cells and their ex vivo expansion

B cells differentiate in human thymus and express AIRE

Neonatal rhinovirus induces mucous metaplasia and airways hyperresponsiveness through IL-25 and type 2 innate lymphoid cells Jun Young Hong, MS, J. Kelley.

Cell-specific activation profile of extracellular signal-regulated kinase 1/2, Jun N-terminal kinase, and p38 mitogen-activated protein kinases in asthmatic.

Is 9 more than 2 also in allergic airway inflammation?

Pathergy response to skin prick testing

Tumor Necrosis Factor-α-Activated Human Adipose Tissue–Derived Mesenchymal Stem Cells Accelerate Cutaneous Wound Healing through Paracrine Mechanisms

Human mast cells arise from a common circulating progenitor

Reversal of atopic dermatitis with narrow-band UVB phototherapy and biomarkers for therapeutic response Suzanne Tintle, BS, Avner Shemer, MD, Mayte Suárez-Fariñas,

Expression of Mas-related gene X2 on mast cells is upregulated in the skin of patients with severe chronic urticaria Daisuke Fujisawa, MD, Jun-ichi Kashiwakura,

Recurrent and persistent respiratory tract viral infections in patients with primary hypogammaglobulinemia Leena Kainulainen, MD, Tytti Vuorinen, MD,

Human IL-31 is induced by IL-4 and promotes TH2-driven inflammation

Lieuwe D. Bos, MSc, PhD, Peter J. Sterk, MD, PhD, Stephen J

TNF-like weak inducer of apoptosis (TWEAK) and TNF-α cooperate in the induction of keratinocyte apoptosis Maya Zimmermann, PhD, Andrea Koreck, MD, Norbert.

Increased expression of CC chemokine ligand 18 in patients with chronic rhinosinusitis with nasal polyps Sarah Peterson, MD, Julie A. Poposki, MS, Deepti.

Programmed cell death ligand 1 alleviates psoriatic inflammation by suppressing IL-17A production from programmed cell death 1–high T cells Jong Hoon.

T-cell involvement in delayed-type hypersensitivity reactions to infliximab Maria Jose Torres, MD, PhD, Patrícia Chaves, PhD, Inmaculada Doña, MD, Natalia.

Tertiary lymphoid organs in recalcitrant chronic rhinosinusitis

Immunofluorescence staining of virus-infected human placental explants

Comèl-Netherton syndrome defined as primary immunodeficiency

Antigen-presenting epithelial cells can play a pivotal role in airway allergy Julia Arebro, MD, Lotta Tengroth, MSc, Ronia Razavi, MSc, Susanna Kumlien.

I-Hsin Kuo, MS, Takeshi Yoshida, PhD, Anna De Benedetto, MD, Lisa A

Profiling of human CD4+ T-cell subsets identifies the TH2-specific noncoding RNA GATA3-AS1 Huan Zhang, MD, PhD, Colm E. Nestor, PhD, Shuli Zhao, PhD,

William J. Calhoun, MD, Tmirah Haselkorn, PhD, Dave P

Potentiation of IL-19 expression in airway epithelia by IL-17A and IL-4/IL-13: Important implications in asthma Fei Huang, PhD, Shinichiro Wachi, PhD,

Reductions in claudin-1 may enhance susceptibility to herpes simplex virus 1 infections in atopic dermatitis Anna De Benedetto, MD, Mark K. Slifka, PhD,

Humanized anti–IFN-γ (HuZAF) in the treatment of psoriasis

Jon Genuneit, MD, MSc Journal of Allergy and Clinical Immunology

Thymic stromal lymphopoietin activity is increased in nasal polyps of patients with chronic rhinosinusitis Deepti R. Nagarkar, PhD, Julie A. Poposki,

Time for a paradigm shift in asthma treatment: From relieving bronchospasm to controlling systemic inflammation Leif Bjermer, MD Journal of Allergy.

Eosinophil production of prostaglandin D2 in patients with aspirin-exacerbated respiratory disease Xin Feng, MD, Madison K. Ramsden, BS, Julie Negri,

Receptor-interacting protein kinase 3 controls keratinocyte activation in a necroptosis- independent manner and promotes psoriatic dermatitis in mice

Ekatherina Vassina, Martin Leverkus, Shida Yousefi, Lasse R

Food allergy in patients with primary immunodeficiency diseases: Prevalence within the US Immunodeficiency Network (USIDNET) Karen S. Tuano, MD, Jordan.

Pulmonary alveolar proteinosis in adenosine deaminase–deficient mice

Increased frequency of dual-positive TH2/TH17 cells in bronchoalveolar lavage fluid characterizes a population of patients with severe asthma Chaoyu.

Glandular mast cells with distinct phenotype are highly elevated in chronic rhinosinusitis with nasal polyps Tetsuji Takabayashi, MD, Atsushi Kato, PhD,

Autophagy: Nobel Prize 2016 and allergy and asthma research

A hypoallergenic cat vaccine based on Fel d 1–derived peptides fused to hepatitis B PreS Katarzyna Niespodziana, MSc, Margarete Focke-Tejkl, PhD, Birgit.

Eosinophil production of prostaglandin D2 in patients with aspirin-exacerbated respiratory disease Xin Feng, MD, Madison K. Ramsden, BS, Julie Negri,

Improvement of treatment-refractory atopic dermatitis by immunoadsorption: A pilot study Michael Kasperkiewicz, MD, Enno Schmidt, MD, PhD, Yvonne Frambach,

The Editors' Choice Journal of Allergy and Clinical Immunology

Benjamin Chaigne, MD, Hervé Watier, MD, PhD

Propagation of respiratory viruses in human airway epithelia reveals persistent virus- specific signatures Manel Essaidi-Laziosi, PhD, Francisco Brito,

Expression of chemokines and chemokine receptors in lesional and nonlesional upper skin of patients with atopic dermatitis Eva Gros, MSc, Caroline Bussmann,

Douglas A. Plager, PhD, Ellen A. Weiss, Gail M. Kephart, BS, Robert M

Biju Thomas, MD, Robert Anthony Hirst, PhD, Mina H

IL-31 is associated with cutaneous lymphocyte antigen–positive skin homing T cells in patients with atopic dermatitis Janine Bilsborough, PhD, Donald.

Immunopathology of atopic dermatitis

Natural history of cow’s milk allergy

Primary prevention of asthma and allergy

Mast cell–associated alveolar inflammation in patients with atopic uncontrolled asthma Cecilia K. Andersson, PhD, Anders Bergqvist, MSc, Michiko Mori,

Presentation transcript:

Rubella persistence in epidermal keratinocytes and granuloma M2 macrophages in patients with primary immunodeficiencies Ludmila Perelygina, PhD, Stanley Plotkin, MD, Pierre Russo, MD, Timo Hautala, MD, PhD, Francisco Bonilla, MD, PhD, Hans D. Ochs, MD, Avni Joshi, MD, MSc, John Routes, MD, Kiran Patel, MD, MS, Claudia Wehr, MD, Joseph Icenogle, PhD, Kathleen E. Sullivan, MD, PhD Journal of Allergy and Clinical Immunology Volume 138, Issue 5, Pages 1436-1439.e11 (November 2016) DOI: 10.1016/j.jaci.2016.06.030 Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig 1 Cutaneous granulomas in patients. A, Hematoxylin and eosin staining of a cutaneous granuloma from case 1. A well-formed granuloma is centrally located. B and C, Cutaneous skin lesions from case 3. Acute and chronic ulcers are observed. D-F, Distribution of RV-infected cells in skin samples of patients with PID. Histological immunofluorescent staining showing focal (Fig 1, D, case 1) or widespread (Fig 1, E, case 2) distribution of RV capsid in granulomas and focal capsid localization in the epidermis (Fig 1, F, case 5). Activation status of macrophages in granulomas (G-I, case 6). Double immunofluorescent staining of granulomas with RV capsid antibody (red) and M2 macrophage-specific antibodies, CD206 (Fig 1, G, green) or CD163 (Fig 1, H, green). I, RV antigen expression in the suprabasal cell layer of skin epidermis (case 1). Double immunofluorescent staining with RV capsid antibody (red) and keratinocyte-specific antibody (cytokeratin, green). Nuclei were counterstained with DAPI. DAPI, 4′-6-Diamidino-2-phenylindole, dihydrochloride; RV-C, rubella virus capsid. Journal of Allergy and Clinical Immunology 2016 138, 1436-1439.e11DOI: (10.1016/j.jaci.2016.06.030) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E1 The genetic relationships between the full rubella genome sequences were inferred using the Neighbor-Joining method. A, The tree is composed of 76 rubella viruses, including sequences of RA27/3 vaccine (bold) and RVs/Oulu.FIN/22.15/PID (red). Sequences were obtained from the GenBank or from Dr Min-hsin Chen. The genetic distances were computed using the maximum composite likelihood method. The scale bar indicates the number of base substitutions per site. The recovered virus sequence is clearly related to the vaccine strain RVI RA27.USA 64 1a vac. B, RV genome organization. The rubella genome is a single-strand RNA virus of positive polarity. The 2 open reading frames are denoted by blue and green boxes. Individual proteins after processing of the 2 polyproteins are shown below. Journal of Allergy and Clinical Immunology 2016 138, 1436-1439.e11DOI: (10.1016/j.jaci.2016.06.030) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E2 RV-positive control slides containing a mixture of 4 human tissues and RV-infected A459 cell double immunostained with capsid mAb and pan-keratin polyclonal antibody and counterstained with DAPI. The slides were either untreated (A) or treated (B) with 0.3% Sudan black solution before mounting. Note the reduction in autofluorescence after Sudan black treatment (Fig E2, B). DAPI, 4′-6-Diamidino-2-phenylindole, dihydrochloride. Journal of Allergy and Clinical Immunology 2016 138, 1436-1439.e11DOI: (10.1016/j.jaci.2016.06.030) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E3 Histological immunofluorescent staining of positive control slides with either rabbit RV polyclonal antibody (CDC) (A), mouse capsid mAb (Abcam) (B), or mouse capsid mAb (CDC) (C), which were preabsorbed with human proteins. Anti-rabbit IgG Alexa Flour-555 (Fig E3, A), anti-mouse IgG Alexa Flour-555 (Fig E3, B), or Alexa Flour-488 (Fig E3, C) served as secondary antibody; nuclei were counterstained with DAPI. DAPI, 4′-6-Diamidino-2-phenylindole, dihydrochloride. Journal of Allergy and Clinical Immunology 2016 138, 1436-1439.e11DOI: (10.1016/j.jaci.2016.06.030) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions

Fig E4 Specificity of rabbit RV polyclonal antibody (CDC). Immunofluorescent staining of Vero cells infected with rubella virus (A and B) with either RV polyclonal or RV polyclonal preabsorbed with purified RV virions. C, Mock-infected Vero cells. Note the lack of specific staining with the antibody preabsorbed with the specific antigen. Journal of Allergy and Clinical Immunology 2016 138, 1436-1439.e11DOI: (10.1016/j.jaci.2016.06.030) Copyright © 2016 American Academy of Allergy, Asthma & Immunology Terms and Conditions