DNA strand synthesis by formation of phosphodiester bonds. The chain is terminated by the use of dideoxycytidine triphosphate (ddC) in place of deoxycytidine triphosphate (dCTP).

Cycle sequencing

One cycle of dye terminator cycle sequencing

one-color/four-lane method such as radioactive sequencing Four-color/one-lane fluorescent sequencing

blue - green -yellow - red Spectral overlap of the dyes in the four virtual filters (vertical gray bars). blue - green -yellow - red 540 nm 570 nm 595 nm 625 nm Example of a matrix

Sequence data obtained from a plasmid with BigDye terminators.