Optimising direct PCR from anagen hair samples Renée Ottens, Duncan Taylor, Damien Abarno, Adrian Linacre  Forensic Science International: Genetics Supplement Series  Volume 4, Issue 1, Pages e109-e110 (January 2013) DOI: 10.1016/j.fsigss.2013.10.056 Copyright © 2013 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Chromatogram of nuclear DNA from a single anagen hair root, amplified using AmpFλSTR® NGM™ kit at 29 cycles on a GeneAmp® System 9600 thermal cycler. Figure shows 8 loci from NGM™ kit. PCR sample was analysed neat and diluted to a concentration of 1:50 before it was injected on an Applied Biosystems 3130xl Genetic Analyser at 3kV for 10s. Red circles indicate split peaks and black squares indicate pull-up in the neat sample. Split peaks and pull-up is not observed in the diluted sample. Forensic Science International: Genetics Supplement Series 2013 4, e109-e110DOI: (10.1016/j.fsigss.2013.10.056) Copyright © 2013 Elsevier Ireland Ltd Terms and Conditions