T.P. Galbraith, R. Harris, P.C. Driscoll, B.A. Wallace

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Presentation transcript:

Solution NMR Studies of Antiamoebin, a Membrane Channel-Forming Polypeptide T.P. Galbraith, R. Harris, P.C. Driscoll, B.A. Wallace Biophysical Journal Volume 84, Issue 1, Pages 185-194 (January 2003) DOI: 10.1016/S0006-3495(03)74841-3 Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 1 13C-1H-HSQC spectrum showing resonances of protons attached to carbon atoms. Enlargement (inset) shows the congested methyl region. Recorded at 298K. Biophysical Journal 2003 84, 185-194DOI: (10.1016/S0006-3495(03)74841-3) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 2 Section of a NOESY spectrum showing inter and intraresidue resonance peaks. Recorded at 278K, 200ms mixing time. Biophysical Journal 2003 84, 185-194DOI: (10.1016/S0006-3495(03)74841-3) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 3 Stereo pictures of the 20 lowest energy conformers, aligned about the main chain atoms in all residues (top), residues 3-8 (bottom left), and residues 9-14 (bottom right). Biophysical Journal 2003 84, 185-194DOI: (10.1016/S0006-3495(03)74841-3) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 4 Stereo views of the lowest energy NMR conformer (cyan) aligned with the crystal structure (red: Snook et al., 1998) about the Cα atoms of residues 1-17 (top), residues 3-8 (bottom left), and residues 9-14 (bottom right). Biophysical Journal 2003 84, 185-194DOI: (10.1016/S0006-3495(03)74841-3) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 5 Bar chart showing temperature coefficients for antiamoebin amide proton chemical shifts, designated by their likelihood of being involved in hydrogen bonding. Black (<2.5 ppb/K) signifies very likely; horizontal stripe (2.5–3.5 ppb/K) signifies likely; dots (3.5–4.0 ppb/K) signifies unlikely; white (>4.0 ppb/K) signifies very unlikely. Biophysical Journal 2003 84, 185-194DOI: (10.1016/S0006-3495(03)74841-3) Copyright © 2003 The Biophysical Society Terms and Conditions

Figure 6 Schematic diagram showing a possible insertion model for antiamoebin in a lipid bilayer (shown in cross section). (A) Monomeric antiamoebin binds to the target membrane. (B) On application of a transmembrane voltage, antiamoebin inserts into the membrane. (C) Antiamoebin monomers assemble to form the active channel. The polypeptide structures shown are Cα backbone worms representing the lowest energy NMR model (cyan: this work) and the crystal structure (red: Snook et al., 1998). Biophysical Journal 2003 84, 185-194DOI: (10.1016/S0006-3495(03)74841-3) Copyright © 2003 The Biophysical Society Terms and Conditions