Molecular surveillance of measles and rubella in the WHO European Region: new challenges in the elimination phase  S. Santibanez, J.M. Hübschen, M.C. Ben Mamou, M. Muscat, K.E. Brown, R. Myers, O. Donoso Mantke, H. Zeichhardt, D. Brockmann, S.V. Shulga, C.P. Muller, P.M. O'Connor, M.N. Mulders, A. Mankertz  Clinical Microbiology and Infection  Volume 23, Issue 8, Pages 516-523 (August 2017) DOI: 10.1016/j.cmi.2017.06.030 Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 1 Time series of MV genotypes in Germany. Each case is depicted by a vertical line. Color differentiates genotypes. In total 2665 cases are depicted for the period Dec. 1999 to Dec. 2016. Clinical Microbiology and Infection 2017 23, 516-523DOI: (10.1016/j.cmi.2017.06.030) Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 2 Phylogenetic relationship between the epidemiologically relevant sequence variants (‘named strains’ in MeaNS) of MV genotype B3. The WHO name, the GenBank accession number (if available) and the ‘distinct sequence identifier’ used in MeaNS are given for each sequence variant. The most prevalent variants in the EUR (≥50 records in MeaNS) are annotated with information on location and dates of first and last detection globally and the number of records submitted globally and by European Region countries (data as 21 June 2017). The phylogenetic analysis is based on the 450-nt sequence encoding the C-terminus of the measles virus N-protein. The tree was constructed using the Neighbor-Joining algorithm and the p-distance method included in MEGA7 [46]. Only bootstrap values (1000 replicates) of at least 60 are shown. The scale bar indicates deviation of 2 nt per 1000-nt sequence. Clinical Microbiology and Infection 2017 23, 516-523DOI: (10.1016/j.cmi.2017.06.030) Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions

Fig. 3 Phylogenetic relationship between the epidemiologically relevant sequence variants (‘named strains’ in MeaNS) of MV genotype D8. The WHO name, the GenBank accession number (if available) and the ‘distinct sequence identifier’ used in MeaNS are given for each sequence variant. The most prevalent variants in the EUR (≥50 records in MeaNS) are annotated with information on location and dates of first and last detection globally and the number of records submitted globally and by European Region countries (data as 21 June 2017). The phylogenetic analysis is based on the 450-nt sequence encoding the C-terminus of the measles virus N-protein. The tree was constructed using the neighbour-joining algorithm and the p-distance method included in MEGA7 [46]. Only bootstrap values (1000 replicates) of at least 60 are shown. The scale bar indicates deviation of 5 nt per 1000-nt sequence. Clinical Microbiology and Infection 2017 23, 516-523DOI: (10.1016/j.cmi.2017.06.030) Copyright © 2017 European Society of Clinical Microbiology and Infectious Diseases Terms and Conditions