Volume 152, Issue 5, Pages 941-943 (April 2017) Cancer Gene Discovery in Hepatocellular Carcinoma: The CRISPR/CAS9 Accelerator  Jean-Charles Nault  Gastroenterology  Volume 152, Issue 5, Pages 941-943 (April 2017) DOI: 10.1053/j.gastro.2017.02.031 Copyright © 2017 AGA Institute Terms and Conditions

Figure 1 Role of the MAPK pathway in liver carcinogenesis. The authors performed genome-wide CRISPR/CAS 9 screening in liver progenitor cells subcutaneously implanted in nude mice. Genetic analysis of the tumor and subsequent in vivo and in vitro experiments validated the role of neurofibromatosis type 1 (NF1) as a tumor suppressor gene, and the RAS/RAF/MAPK pathway as a key signaling pathway in hepatocellular carcinoma (HCC) development. Percentages of mutations in human HCC are shown in blue for tumor suppressor genes and red for oncogenes; potential biotherapy is shown in purple. Gastroenterology 2017 152, 941-943DOI: (10.1053/j.gastro.2017.02.031) Copyright © 2017 AGA Institute Terms and Conditions