Conjunctival Impression Cytology in healthy volunteers

Slides:

Advertisements

Similar presentations

Ocular Surface Diseases

Advertisements

Contact lenses Week 2.  Tear film consists of three layers with a pH level of 7.3 and a salt concentrations of 0.91 to0.97%. Normal evaporation is 1.

ASCRS Symposium & Congress - San Diego 2011

Sérgio Kwitko, Tiago Lansini, Andressa P Stolz, Diane R Marinho Authors have no financial interest in the subject matter of this poster.

World Cornea Congress VII Loteprednol Etabonate 0.5% Versus Cyclosporine 0.05% for Dry-Eye Syndrome After Bone Marrow Transplantation: A Prospective Randomized.

‘‘DRY EYE’’. Dry Eye Dry eye is a disease of the ocular surface attributable to different disturbances of the natural function and protective mechanisms.

Incidence of Blepharitis in Patients Undergoing Phacoemulsification Jodi Luchs, MD Carlos Buznego, MD William Trattler, MD The authors of this poster have.

Delay in Symptom Resolution After Thermal Pulsation System Treatment for Meibomian Gland Dysfunction in Dry-Eye Disease Delay in Symptom Resolution After.

Non-Invasive Keratograph Tear Break-Up Time versus Invasive Fluorescein-Associated Tear Stability Test: Experimental Validation Study Nizar S Abdelfattah,

Impact of Conjunctivochalasis in Dry Eye

Epitheliopathy in the Region of the Eyelid Associated with the Caruncle in Normal Subjects and Dry-Eye Patients Epitheliopathy in the Region of the Eyelid.

Yousef A Alghamdi, MD; Allison L McClellan, OD; Nabeel M Shalabi, MD Anat Galor, MD, MSPH Supported by the Department of Veterans Affairs, Veterans Health.

Young Joo Shin 1, Joo Hyun Kim 1, Jung Ha Kim 2, Woo Ho Nam 1, Kayoung Yi 1, Joon Young Hyon 3, 1 Department of Ophthalmology, Hallym University College.

W. B. Trattler; C. D. Reilly; D. F. Goldberg; P. A. Majmudar; J. A. Vukich; M. Packer; E. D. Donnenfeld.

Blepharitis and Dry eyes in Aromatase Inhibitor Users Kiran Turaka, M.D. Kristin M. Hammersmith, M.D. Jennifer M. Nottage, M.D. Christopher J. Rapuano,

Staining Patterns in Dry Eye Syndrome: Rose Bengal Versus Trypan Blue Rosane S. Castro (1) Lívia M. D. Freire (1), Renato Ambrosio Jr(2) 1-Ophthalmology.

Use of fibrin glue in ocular surgery

Copyright restrictions may apply Sensitivity and Specificity of a Point-of-Care Matrix Metalloproteinase 9 Immunoassay for Diagnosing Inflammation Related.

Association between Depression And Dry eye Sang Beom Han, MD, 1 Joon Young Hyon, MD, 1 Young Joo Shin, MD, 1 Won Ryang Wee, MD, 2 Jin Hak Lee, MD, 1 1.

Blepharitis and Dry eyes in Aromatase Inhibitor Users

Copyright restrictions may apply JAMA Ophthalmology Journal Club Slides: Effects of Azithromycin in Patients With Meibomian Gland Disease Zhang L, Su Z,

Changes of Ocular Surface in a Rabbit Model of Short Term Desiccating Stress Wei-Li Chen, MD, PhD Associate Professor, National Taiwan University Hospital.

Evaluation of Epithelial Changes in Limbal Stem Cell Deficiency Using in Vivo Confocal Microscopy ERIC CHAN, Luxia Chen, Sophie X. Deng Cornea and Uveitis.

A Novel Technique to Diagnose and Follow up Conjunctival and Corneal Intraepithelial Neoplasia Using Ultra High Resolution Optical Coherence Tomography.

Topical Tacrolimus 0,03% for Treatment of Refractory Allergic Conjunctivitis Diane Marinho, Andréia Ferreira Laranjeira, Sérgio Kwitko, Samuel Rymer Hospital.

Ten-year Clinicopathological Review Of Ocular Surface Squamous Neoplasia In An Ophthalmological Center In Mexico City Lucero Pedro-Aguilar, M. D. Alvarez-Melloni.

Blepharitis and Dry eyes in Aromatase Inhibitor Users Kiran Turaka, M.D. Kristin M. Hammersmith, M.D. Jennifer M. Nottage, M.D. Christopher J. Rapuano,

14 cases of keratoconjunctival tumor Satoru Tsuda, Shunji Yokokura, Akira Kubota, Megumi Uematsu, and Kohji Nishida Department of Opthalmology and Visual.

The authors have no financial interest

World Cornea Congress VI Michael K. Smolek & Panagiotis Kompotiatis Department of Ophthalmology LSU Eye Center of Excellence, New Orleans The authors.

FLUORESCEIN CLEARANCE TEST AS AN ADDITIONAL DIAGNOSTIC TOOL FOR DYSFUNCTIONAL TEAR SYNDROME Rodrigo de Nápole Azevêdo, MD Jamília Barros da Silva, MD Aline.

Quantification of Soluble Cytokines in Sjögren Disease, non Sjögren dry eye and Healthy Subjects M. Jaimes 1B, C. Santacruz-Valdes 1B, Y. Garfias 1A, T.

Sergio Kwitko, Andressa P. Stolz, Melissa Dal Pizzol, Diane Marinho, Samuel Rymer Ophthalmology Department - Hospital de Clínicas de Porto Alegre Federal.

Prevalence of Dry Eye Disease among Elderly Korean Population Sang Beom Han, MD, 1 Joon Young Hyon, MD, 1 Won Ryang Wee, MD, 2,3 Jin Hak Lee, MD, 1, 3.

Correlation of Conjunctival and Corneal Staining With Elevated Matrix Metalloproteinase-9 on the Ocular Surface of Dry-Eye Patients Authors: Allister Gibbons,

STAINING R.SEENIAMMAL AEH, THENI.

Ancillary and Lab test. Basic eye examination Test Snellen visual acuity. Look for conjunctival hyperemia, chemosis, superior and inferior subconjunctival.

Diane Marinho, Cláudia Borowsky, Carlos Galia Ophthalmology Department - Hospital de Clínicas de Porto Alegre Federal University of Rio Grande do Sul -

Comparison of Efficacy of 0.05% Cyclosporine Ophthalmic Emulsion

1 Conjunctival Goblet cell Density Following Sequential Therapy With Artificial Tear and Cyclosporine 0.05% Frank A. Bucci, Jr, MD 1 ; Stephen C. Pflugfelder,

Diagnosis and Management of Conjunctival Leiomyosarcoma Matthew D. Council, George J. Harocopos, Andrew J. Huang A. Huang is a consultant speaker for Allergan,

PTERYGIUM PROGRESSION DETECTED BY IN VIVO CONFOCAL MICROSCOPY Martone Gianluca, Malandrini Alex, Balestrazzi Angelo, Tosi Gian Marco, Pichierri Patrizia,

The Course of Dry Eye After Phacoemulsification Surgery Servet Cetinkaya 1, Emine Mestan 2, Nursen Oncel Acir 3, Yasemin Fatma Cetinkaya 4, Zeynep Dadaci.

Karin Spielmann & Lars Affentranger. Study aim To explore, if the sensitivity of the lid margins at the level of the lidwiper decreases with age.

1. Ankara University School of Medicine, Department of Ophthalmology

Limited Scleroderma (CREST Syndrome) is associated with worse

RF-OCS-111 SOE 2017, June Presented By Dr. Joobin Khadamy

Dry Eye & Schirmer’s Test

From: Lower Vitamin D Level and Distinct Tear Cytokine Profile Were Observed in Patients with Mild Dry Eye Signs but Exaggerated Symptoms Trans. Vis. Sci.

Invest. Ophthalmol. Vis. Sci ;49(1): doi: /iovs Figure Legend:

Christina K. Marko, Balaraj B. Menon, Gang Chen, Jeffrey A

EVALUATION OF OCULAR SURFACE DISEASE IN PATIENTS AFTER TRABECULECTOMY: COMPARATIVE STUDY WITH AND WITHOUT INTRACAMERAL BEVACIZUMAB Sónia Parreira; Sandra.

CORNEAL EPITHELIAL IMAGING

Prevalence of Symptomatic Dry Eye Disease in an Urban Chinese Community: A Cross-sectional Population-based Study Venice SW LI, Sophie SL YEOW, Jeffrey.

Copyright © 2002 American Medical Association. All rights reserved.

L02: Tear break up time (BUT)

Cytokine Production and Antigen Recognition by Human Mucosal Homing Conjunctival Effector Memory CD8+ T Cells Invest. Ophthalmol. Vis. Sci ;55(12):

Conjunctival Goblet Cell Density Following Cataract Surgery With Diclofenac Versus Diclofenac and Rebamipide: A Randomized Trial Kumiko Kato, Kensaku.

Volume 119, Issue 10, Pages (October 2012)

Jodi Luchs, MD Carlos Buznego, MD William Trattler, MD

A presentation to: Meeting name Date

Yumiko Ban,1,2,3) Seika Den,2) Jun Shimazaki,2,3)

Oxidative Balance of Autologous Serum Eyedrops

JAMA Ophthalmology Journal Club Slides: Clinical Features of Benign vs Malignant Conjunctival Tumors in Children Shields CL, Sioufi K, Alset AE, et al.

The authors have no financial interests to disclose

JAMA Ophthalmology Journal Club Slides: Effects of Azithromycin in Patients With Meibomian Gland Disease Zhang L, Su Z, Zhang Z, Lin J, Li D-Q, Pflugfelder.

Punctual plug and dry eye after photorefractive keratectomy

I-Chan Lin MD, Paul P. Lee MD, JD

Cell characteristics after treatment with either sodium hyaluronate or saline. Cell characteristics after treatment with either sodium hyaluronate or saline.

Presentation transcript:

Conjunctival Impression Cytology in healthy volunteers Diane Marinho, Andressa P. Stolz, Sergio Kwitko, Felipe K. Mallmann, Samuel Rymer Ophthalmology Department - Hospital de Clínicas de Porto Alegre Federal University of Rio Grande do Sul - Porto Alegre - Brazil The authors have no financial interest in the subject matter of this poster

Purpose To determine conjunctival cytology findings in volunteers with no history of ocular disease and to correlate results with dry eye tests and frequency of symptoms.

Methods Impression cytology (IC) of superior, inferior, nasal and temporal conjunctivae (Aragona’s scoring system) Ocular surface evaluation clinical tests: Schirmer test (ST), tear film break-up time (TFBUT), Fluorescein staining (FS), Rose Bengal staining (RBS) and slit lamp examination History and symptoms questionnaire

Results 78 eyes of 39 patients (77% female, 85% white) were examined. Mean age was 362 years (16-71) Impression cytology : - grade A (normal): 71% superior 57% nasal 87% inferior 75% temporal Snake-like chromatin in 15% of superior samples. Clinical Tests: Mean BUT was 13.6 seconds (70% ≥10 sec) ST median was 19.2 mm (76% >6 mm), RBS grade 0-3 in 87% FS grade 0 in 75% Slit lamp eyelid exam normal in 65%

Table 01. Scoring system for conjunctival impression cytology Parameters Normal Borderline Abnormal Cellularity 1 2 Cell-to-cell contact Nucleus/cytoplasm ratio 0 (1:2-3) 1 (1:4-5) 2 (1:≥ 6) Nuclear chromatin 1 (≤ 1%)* 2 (> 1%)* Goblet cell distribution Keratinisation 2-3 Inflammatory cells - Total Score 0-3 (class A) 4-6 (class B) > 6 (class C) *if snake-like chromatin

Figure 01. Illustrative impression cytology specimens B C D A. Normal Cytology, B. Borderline Cytology, arrow indicating goblet cell, C. Abnormal Cytology (superior conjunctiva, PAS/HE/Papanicolaou OM 200x), D. 1-10% Snake-like chromatin containing sample, sign indicating a track of anomalous nuclear cells and E. > 10% Snake-like chromatin containing sample (superior conjunctiva, PAS/HE/Papanicolaou OM 400x). E

Results Nasal IC was associated with ST, TFBUT, RBS and symptoms. Nasal IC score showed positive correlation with number of abnormal clinical tests (figure 3). Temporal IC was associated with ST and symptoms. Snake-like chromatin was associated with age, ST and IC nasal and superior findings.

Figure 02. IC data (valid specimens) for 6 combined clinical tests *Linear-by-linear association 0.001. S = superior; N = nasal; I = inferior; T = temporal.

Conclusions Our study identified a prevalence of 57 – 81% of normal cytological findings in normal subjects Impression cytology findings of exposed bulbar conjunctiva were more often altered Despite a few associations among tests, there was no conclusive correlation among these tests, symptoms and IC results.

References Aragona P, Romeo GF, Puzzolo D, Micali A, Ferreri G. Impression cytology of the conjunctival epithelium in patients with vernal conjunctivitis. Eye 1996;10 ( Pt 1):82-5. Nelson JD. Impression cytology. Cornea 1988;7:71-81 Schein OD, Tielsch JM, Munoz B, Bandeen-Roche K, West S. Relation between signs and symptoms of dry eye in the elderly. A population-based perspective. Ophthalmology 1997;104:1395-401. Methodologies to diagnose and monitor dry eye disease: report of the Diagnostic Methodology Subcommittee of the International Dry Eye WorkShop (2007). Ocul Surf 2007;5:108-52. Bjerrum KB. Snake-like chromatin in conjunctival cells of a population aged 30-60 years from Copenhagen City. Acta Ophthalmol Scand 1998;76:294-8. Paschides CA, Petroutsos G, Psilas K. Correlation of conjunctival impression cytology results with lacrimal function and age. Acta Ophthalmol (Copenh) 1991;69:422-5.