Molecular Oncology Testing in Resource-Limited Settings

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Presentation transcript:

Molecular Oncology Testing in Resource-Limited Settings Margaret L. Gulley, Douglas R. Morgan The Journal of Molecular Diagnostics Volume 16, Issue 6, Pages 601-611 (November 2014) DOI: 10.1016/j.jmoldx.2014.07.002 Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 Infection-related cancers comprise 23% of all cancers worldwide. Oncogenic pathogens (A) are linked to higher cancer burden in less developed regions of the world (B). Data are from de Martel et al.3 HTLV1, human T-cell lymphotropic virus 1. The Journal of Molecular Diagnostics 2014 16, 601-611DOI: (10.1016/j.jmoldx.2014.07.002) Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 2 Devices initially developed for military or aerospace use can be re-appropriated for laboratory medicine. A portable thermocycler with laptop computer interface, initially developed for military field work, was later adapted for health care settings. The military version, dubbed the ruggedized advanced pathogen identification device (RAPID), was designed, in part, to examine environmental samples for biowarfare agents. The device requires limited space and technologist expertise, while still providing reliable real-time molecular results. Image used with permission of BioFire Defense. The Journal of Molecular Diagnostics 2014 16, 601-611DOI: (10.1016/j.jmoldx.2014.07.002) Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 3 Electronic biosensors operate by electrochemical signal transduction on hybridization to complementary target sequence in the patient specimen. In this example, a hybridization-induced conformation change increases the distance between the electrode and the reporter molecule, which alters electron-transfer dynamics to generate a current in the electrode. Billions of sensors can be placed on a single semiconductor chip for simultaneous analysis of all relevant targets in human and pathogen genomes. System parts (chips, reagents, and detectors) are all inexpensive. The Journal of Molecular Diagnostics 2014 16, 601-611DOI: (10.1016/j.jmoldx.2014.07.002) Copyright © 2014 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions