Validation of forensic body fluid identification based on empirically normalized miRNA expression data  Eva Sauer, Ann-Kathrin Reinke, Cornelius Courts  Forensic Science International: Genetics Supplement Series  Volume 5, Pages e462-e464 (December 2015) DOI: 10.1016/j.fsigss.2015.09.183 Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Schematic representation of the decision tree developed to distinguish between the five forensically relevant body fluids venous blood, saliva, semen, vaginal secretion and menstrual blood. The scatterplots in the lower panel represent the ΔCq-values of two differentially expressed miRNAs each to distinguish between saliva and vaginal secretion and venous and menstrual blood, respectively. Left: x-axis: ΔCq-values of hsa-miR-203a-3p, y-axis: ΔCq-values of hsa-miR-124-3p, circles represent saliva samples (n=15), triangles represent vaginal secretion samples (n=15); right: x-axis: ΔCq-values of hsa-miR-144-3p, y-axis: ΔCq-values of hsa-miR-203a-3p, diamonds represent venous blood samples (n=15), squares represent menstrual blood samples (n=15); the dotted lines represent the respective linear decision boundaries. Forensic Science International: Genetics Supplement Series 2015 5, e462-e464DOI: (10.1016/j.fsigss.2015.09.183) Copyright © 2015 Elsevier Ireland Ltd Terms and Conditions