Table 1: Efficiency for target genes

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Table 1: Efficiency for target genes Table 1: Efficiency for target genes. Efficiency was calculated as 10 (-1/Slope) -1 TARGET GENE RNA SAMPLE SLOPE EFFICIENCY e-NOS Basal -3.411 96.4 % ME -3.427 95.8 % 1 h -3.398 96.9 % 2 h -3.431 95.6 % 3 h -3.444 95.1 % n-NOS -3.451 94.8 % -3.437 95.4 % -3.418 96.1 % -3.464 94.4 % i-NOS -3.384 97.4 % -3.378 97.7 % -3.356 98.5 % -3.390 97.2 % -3.369 98.1 % 20 Table 2. Means ± SEM of CT value for G3PDH housekeeping gene tested in the samples used 19.5 19 y = 0,045x + 18,307 Gene Expression Index (CT) 18.5 samples G3PDH CT mean CT SEM BASAL 18.50138 0.17324 ME 18.38321 0.10606 1h 17.28167 0.09828 2h 18.27271 0.16263 3h 18.78507 0.21778 18 17.5 17 16.5 16 B ME 1h 2h 3h samples Figure 1: Regression lines for G3PDH gene showing predicted regression lines and actual means. G3PDH has low slope and close fit to the regression line.