Genomics, Proteomics & Bioinformatics ABS-932 Introductory Lecture Powerpoint Templates

Why was this course proposed? Genomics and Proteomics are the study of genome and proteome structure and function, respectively. These are the new and exciting areas which has recently witnessed many conceptual and technical advances. This information is vital to our day-to-day living in this century.

Course Description The main purpose of this course is to introduce concepts of human genomics and proteomics that can also be applied to microbial, plant and animal genomes/ proteomes. The topics covered are: Introduction to genomics/proteomics Genomes annotation Overview of genomics/proteomics projects Transcriptomics Metabolomics Protein structure/function and Insilico Prediction Homology modeling Data mining Applications of bioinformatics

Essential Details Credits: 3 Time: Monday and Friday 09.30 -11.00 hrs Place: Classroom 307

Grades Course point distribution Class presentations/assignments 10% Quizzes 10% OHT 30% (2 OHTs) End Term Exam 50%

keep your cell phones on silent mode or put them off. RULES Plan to arrive to class on time and to stay for the entire class period (or until dismissed). NO will be allowed to enter/leave while class is in session Talking and other disruptive behaviors are not permitted while class is in session. Pens and pencils are distracting. NO tapping, clicking, flipping and rolling. keep your cell phones on silent mode or put them off.

What is the simplest definition of genomics? What is the genome? How many types of genomes are there in this world?

Why should we study genomes? Each and everyone is a unique creation! Life’s little book of instructions DNA blue print of life! Human body has 1013 cells and each cell has 6 billion base pairs (A, C, G, T) A hidden language/code determines which proteins should be made and when This language is common to all organisms

What can genome sequence tell us? Everything about the organism's life Its developmental program Disease resistance or susceptibility History

How will we change in this century because of the Genomics? You will control the destiny of this planet Big changes in our own life Biotechnology: more products GMOs: More food-More problems? Our society will not be the same! Individualized medicine Gene therapy Immortality? Disease free life?

What genomics has to do with me? Wild type What genomics has to do with me?

Why horse is a horse and duck is a duck? It is in their genes! DNA structure was discovered in 1953 DNA replicates by making a copy of itself and passes to next generation of cells or organisms Purity of lineages maintained Biotechnology: fish genes in plants

Origin of terminology The term genome was used by German botanist Hans Winker in 1920 Collection of genes in haploid set of chromosomes Now it encompasses all DNA in a cell In 1986 mouse geneticist Thomas Roderick used Genomics for “mapping, sequencing and characterizing genomes” New terms: Functional genomics, transcriptomics, proteomics, metabolomics, phenomics (Omics)

Origin of Genomics Human Genome Project Goal: sequence 3 billion base pairs High-quality sequence (<1 error per 10 K bases) ACGT Immensity of task required new technologies Automated sequencing Decision to sequence other genomes: yeast and bacteria Beginnings of comparative genomics 15

Technical foundations of genomics Log MW Distance . Molecular biology: recombinant-DNA technology DNA sequencing Library construction PCR amplification Hybridization techniques 16

Genomics relies on high-throughput technologies 200 Automated sequencers Fluorescent dyes Robotics Microarray spotters Colony pickers High-throughput genetics 17

Bioinformatics: computational analysis of genomics data Uses computational approaches to solve genomics problems Sequence analysis Gene prediction Modeling of biological processes/ network 18