Allergy to latex, avocado pear, and banana: Evidence for a 30 kd antigen in immunoblotting François Lavaud, MD, Alain Prevost, MD, Claude Cossart, MD,

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Presentation transcript:

Allergy to latex, avocado pear, and banana: Evidence for a 30 kd antigen in immunoblotting François Lavaud, MD, Alain Prevost, MD, Claude Cossart, MD, Laurence Guerin, PhD, Jacky Bernard, PhD, Serge Kochman, MD Journal of Allergy and Clinical Immunology Volume 95, Issue 2, Pages 557-564 (February 1995) DOI: 10.1016/S0091-6749(95)70318-7 Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 1 SDS-PAGE, 15%, stained with silver nitrate. Lane 1, Latex glove extract; lane 2, fresh serum latex extract; lane 3, avocado pear extract; lane 4, banana extract. Each extract contains approximately 300 μg of protein. MW, Protein molecular weight markers. Journal of Allergy and Clinical Immunology 1995 95, 557-564DOI: (10.1016/S0091-6749(95)70318-7) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 2 IgE immunoblotting with patients' sera. Selected patterns of sera from patients with latex allergy without (no. 15) or with an associated fruit allergy (nos. 32 to 35). B, Banana; A, avocado pear; L, natural latex. Journal of Allergy and Clinical Immunology 1995 95, 557-564DOI: (10.1016/S0091-6749(95)70318-7) Copyright © 1995 Mosby, Inc. Terms and Conditions

FIG. 3 Representative immunoblot patterns of reactivity observed in inhibition studies. Serum from patient 35 was preincubated with 50 μg each of banana (first panel), avocado pear (second panel), and latex extract (third panel), then tested in immunoblot versus banana (lane B), avocado pear (lane A) , and latex (lane L). Lane C shows the initial pattern without preincubation. Journal of Allergy and Clinical Immunology 1995 95, 557-564DOI: (10.1016/S0091-6749(95)70318-7) Copyright © 1995 Mosby, Inc. Terms and Conditions