Lesson 054 Learning Goal: (You should be able to…) Describe the affect of chromatography on ink : (Can you …) Make a chromatogram? Obtain data from a chromatogram?

In your lab notebook lesson 054 Data in lab note book Chromatogram for brown ink

Vocabulary for solubility and chromatography Solution Solvent Solute Suspension Colloid Mixture medium in chromatography Dissolve Solubility Rate of solubility Capillary action Absorption/absorb

You need your Lab Notebook Write To Think 054 You need your Lab Notebook Read the AQUA sheet “Procedure for making a chromatogram” Study the diagram about how to set up a chromatography tank and make a chromatogram. You have about 4 minutes. Your teacher will then demonstrate how to make a chromatogram and explain why it’s useful and what you’ll use it for. Put a title in your Lab notebook: Chromatography

You need your Lab Notebook Write To Think 054 You need your Lab Notebook Read the AQUA sheet “Procedure for making a chromatogram” Study the diagram about how to set up a chromatography tank and make a chromatogram. You have about 4 minutes. Your teacher will then demonstrate how to make a chromatogram and explain why it’s useful and what you’ll use it for. 3. Put a title in your Lab notebook: Chromatography

Plan of Action for today How to set up a chromatography ‘tank’ Make a chromatogram (s) Record qualitative and quantitative data Debrief questions etc.

Chromatography A method of separating the parts of a mixture using the different solubility of chemicals and the process of capillary action using a solvent (water) and a medium (paper towel). It produces a colored (chrom) ‘picture’ (graph) It is useful for identifying unknown chemicals by comparing the distance travelled and time taken with standard or baseline data of known chemicals. FYI: Solubility is a physical property FYI: Capillary action is a physical process

Instructions and demonstration Backpacks, lab notebooks, etc. Bench lay out … same at the end please Equipment/materials … a set PER PERSON (scissors) Take it all out (Paper towel … keep it clean!!) Reliable, consistent, repeatable … because … How to … demonstration and tips

Materials per person Large chromatography tank Small water (solvent) reservoir/beaker Stirring rod (medium support) Paper clip Paper towel (medium) Stopwatch/timer 15 cm Ruler Scissors

Chromatography tank set up Stir rod Paper clip Paper towel Pencil line Brown marker spot Water See next slide before starting!

Collecting and Recording data Time how long the ink takes to travel up the paper towel (medium) until you stop it by taking it out of the water (solvent). You decide when to take it out … but not too late! Make a pencil mark CAREFULLY where the solvent reaches … do this IMMEDIATELY as the solvent will keep moving! Measure the distance (cm) the INK travelled and solvent travelled from the original spot. IN YOUR LAB NOTEBOOK Title Chromatography Record qualitative (what you see, description) and quantitative (what you measured, time, minutes, distance, cm) data Scotch tape the chromatogram into your lab note book and label it. (It’s wet … so don’t tear it!)

Debrief Questions What happened to the brown ink? Why? How? What does it imply? Infer? What was the rate of movement? (Velocity!) Do you need to change the water each time? Explain.

Design flaws and human mistakes What are the possible human mistakes you could/did make? (What went ‘wrong’ or could go ‘wrong’?) What are the design flaws with the chromatography set up? What might affect the data? What could affect the consistency of the data? If you did this again with the SAME marker would you get the same data? What must you do to make sure? How will you be consistent?

Variables and Constants Possible Independent Variables, manipulated (IV): Dependent Variables, responding (DV): Constants, stays the same each time:

Summary 3 factors that might affect the rate of chromatography. 2 dependent variables that can be measured in chromatography. 1 reason chromatography is useful.

End of lesson

Plan of Action … PUT IN Lesson 055 Day 1: Monday 10/31: How to make a chromatogram. Make one or two. Collect data. (Quantitative and Qualitative.) What’s happening? Day 2: Tuesday 11/01: Graffiti crime scene. Look at TPD data. Set up LNB and plan. Hypothesis. What to do if … Day 3: Wednesday (34 min) 11/02: Collect data part 1 … short amount of time! Day 4: Thursday (Mr. Thompson out) 11/03: Read about chromatography. Answer questions to help with understanding of how it works. Write hypothesis. What is solubility? What is capillary action? Day 5: Friday 11/04: Collect data part 2 Day 6: Monday 11/07: Collect data part 3. Data organization and data analysis Day 7: Tuesday 11/08: Data organization and data analysis. Day 8: Wednesday (52 min … no late start) 11/09: Conclusion (CER) part 1 Day 9: Thursday 11/10: Conclusion (CER) part 2 MUST FINISH by end of DAY! No school Friday 11th

Investigative question How are different inks affected by chromatography? Plan of Action for today: How to set up a chromatography ‘tank’ Make a chromatogram and record data Identify possible experimental flaws (and human mistakes) Write an hypothesis

You’ll investigate … Three colored inks BLUE YELLOW BLACK