Cdc42.GTP directly activates PAK4.

Slides:

Advertisements

Similar presentations

Volume 7, Issue 3, Pages (May 2014)

Advertisements

Volume 16, Issue 6, Pages (August 2016)

Implications of somatic mutations in the AML1 gene in radiation-associated and therapy-related myelodysplastic syndrome/acute myeloid leukemia by Hironori.

Plk1 Controls the Nek2A-PP1γ Antagonism in Centrosome Disjunction

CDC20 interacts with conductin and induces its proteosomal degradation

Yeast Tgl3p expressed in HeLa cells localizes to lipid droplets.

JNK is activated by Wnt5a.

SERCA2 is a functional adaptor for the alternative toll‐like receptor 9 (TLR9) signalling in cardiomyocytes Co‐immunoprecipitated TLR9 with SERCA2 antibody.

by Ji-Long Chen, Andre Limnander, and Paul B. Rothman

1,25‐dihydroxyvitamin D3 and retinoic acid induce p19ink4d expression.

The N‐terminus and SH2 domain of SOCS‐1 contribute to inhibition of JAK1 and JAK2 kinase activity. The N‐terminus and SH2 domain of SOCS‐1 contribute to.

Calcium regulates ERK nuclear association, but not its activation.

Large Hepatitis Delta Antigen Modulates Transforming Growth Factor-β Signaling Cascades: Implication of Hepatitis Delta Virus–Induced Liver Fibrosis

Wild‐type p53 binds and represses Rad51 promoter.

TRF2/RAP1complex mediates C‐NHEJ inhibition at telomeric ends through hindrance at the KU loading and DNA–PK activation steps. TRF2/RAP1complex mediates.

TGF-β1 induces ILK activity in renal tubular epithelial cells.

AO domain of LSD1 is responsible for its interaction with Snail1.

Mitophagy is independent of PINK1 and Parkin.

SkMLCK physically interacts with and phosphorylates MEF2C in vivo and in vitro. skMLCK physically interacts with and phosphorylates MEF2C in vivo and in.

Interaction of xFRS2 with the Src family kinase Laloo.

c‐Abl acetylation on Lys 730 promotes myogenic differentiation.

Volume 33, Issue 2, Pages (January 2009)

MyoD is acetylated on lysines 99 and 102 in myoblasts.

Nore1 enhances interaction between HIPK1 and Mdm2.

Ubiquitination of Drp1 by MARCH‐V.

RYBP binds to MDM2. RYBP binds to MDM2. (A) COS7 cells (5 × 105 cells per well) were transfected with 10 μg of T7‐MDM2, Myc‐RYBP or both for 24 h. The.

Recruitment of ISWI and the NURF complex by Armadillo.

Interaction of human trimethylguanosine synthase 1 with the carboxyl tail of SmB and the survival of motor neuron protein. Interaction of human trimethylguanosine.

Mutations affecting HOIP–UBA and HOIL‐1L–UBL interaction that compromise NF‐κB activation. Mutations affecting HOIP–UBA and HOIL‐1L–UBL interaction that.

The scaffold protein APPL1 facilitates HDAC3 regulation of AKT

Volume 22, Issue 5, Pages (May 2012)

Npl3 functions as an independent export adaptor for the pre‐60S ribosomal subunit. Npl3 functions as an independent export adaptor for the pre‐60S ribosomal.

MiR‐199a directly targets ERBB2 and ERBB3, whereas miR‐125b targets ERBB3. miR‐199a directly targets ERBB2 and ERBB3, whereas miR‐125b targets ERBB3. (A)

A Rac-cGMP Signaling Pathway

The MSP domain of MOSPD2 is sufficient to interact with STARD3 and STARD3NL The MSP domain of MOSPD2 is sufficient to interact with STARD3 and STARD3NL.

Palbociclib activates the proteasome indirectly and reduces the association of ECM29 with the proteasome Palbociclib activates the proteasome indirectly.

The FKBP8‐ATG8 interaction is dependent on an intact LIR docking site (LDS)‏ The FKBP8‐ATG8 interaction is dependent on an intact LIR docking site (LDS)

KLK7 is involved in the Aβ degradation activity

Beclin‐1 depletion reduces targeting of several outer kinetochore proteins. Beclin‐1 depletion reduces targeting of several outer kinetochore proteins.

Communication with the Exon-Junction Complex and Activation of Nonsense-Mediated Decay by Human Upf Proteins Occur in the Cytoplasm Guramrit Singh, Steffen.

Effect of HUWE1 on MYC and MIZ1 complexes Immunoblot documenting levels of MYC and MIZ1 upon HUWE1 overexpression. Effect of HUWE1 on MYC and MIZ1 complexes.

Grb2 Is a Key Mediator of Helicobacter pylori CagA Protein Activities

Volume 7, Issue 3, Pages (May 2014)

NuMA directly interacts with Plk1 and gets phosphorylated at its C-terminus. NuMA directly interacts with Plk1 and gets phosphorylated at its C-terminus.

WDR11 forms a tertiary complex with EMX1 and GLI3

Usp5 sensitivity of S65 ubiquitin mutant chains Structure of ubiquitin in complex with Usp5 (PDB ID: 3IHP). Usp5 sensitivity of S65 ubiquitin mutant chains.

Plk1 Controls the Nek2A-PP1γ Antagonism in Centrosome Disjunction

Tsc1 co‐chaperone inhibits Hsp90 chaperone function

Volume 93, Issue 5, Pages (May 1998)

The subcellular localization, dimerization with Arnt and DNA‐binding activity of AhR are altered by the activation of Gα13. The subcellular localization,

Activated Gα13 inhibits AhR‐mediated transcriptional activity.

Volume 22, Issue 5, Pages (January 2018)

NDP52 promotes CHIKV infection in a species‐specific manner.

Lysine 63 Polyubiquitination of the Nerve Growth Factor Receptor TrkA Directs Internalization and Signaling Thangiah Geetha, Jianxiong Jiang, Marie W.

Volume 9, Issue 4, Pages (April 2011)

Soluble TLR4 can block Ni2+/Co2+‐induced IL‐8 production.

Sandrine Etienne-Manneville, Alan Hall Cell

Volume 52, Issue 2, Pages (October 2013)

The PAR-6 Polarity Protein Regulates Dendritic Spine Morphogenesis through p190 RhoGAP and the Rho GTPase Huaye Zhang, Ian G. Macara Developmental Cell

Ubqln1 interacts with Ubqln4.

Volume 62, Issue 4, Pages (May 2016)

RIF1 interacts with protein phosphatase 1 isoforms

Fig. 7. Ror-GFP binds to Myc-tagged Wnts and Wnt receptors.

Figure 4 DNM1 mutations affect protein levels and self-dimerization (A) HeLa cells were transfected with green fluorescent protein (GFP)-tagged mutant.

MiR-431-5p directly targets RAF1 and is negatively correlated to RAF1 expression (A) The predicted binding sites of miR-431-5p and RAF1 3′-UTR, and mutant.

GOLPH3 interacts with COG complex proteins.

Grb2 Is a Key Mediator of Helicobacter pylori CagA Protein Activities

PKM2 is tyrosine phosphorylated and inhibited by FGFR1 in cancer cells with oncogenic or overexpressed FGFR1. PKM2 is tyrosine phosphorylated and inhibited.

Key functional sites of SPINDLIN1 could be phosphorylated by Aurora-A.

Volume 57, Issue 2, Pages (January 2015)

Presentation transcript:

Cdc42.GTP directly activates PAK4. Cdc42.GTP directly activates PAK4. (A) A schematic of the pull‐down protocol from COS7 cell lysates is shown. The PAK4 proteins were recovered as indicated, and assayed for activity towards glutathione S‐transferase (GST)–Raf13 (20 μg) in the presence of 10 μM [γ32‐P] ATP. The pS474 was probed using CS#3241. (B) Schematic of the active Cdc42–Gly6–PAK4 chimaera (Flag‐C–PAK4). (C) The relative in vitro kinase activity of purified PAK4 tested in three independent experiments was calculated after subtracting PAK4 KD (that is, background value) and setting the Flag–PAK4 value to 1. The bars represent the standard error for each measurement. (D) The efficiency of green fluorescent protein–GEFH1 binding to various Flag–PAK4 constructs as indicated was tested by coimmunoprecipitation. CA, constitutively active; Cat, PAK4(286–591); CRIB, Cdc42–Rac interaction/binding; GFP, green fluorescent protein; GST, glutathione S‐transferase; HA, haemagglutinin; IP, immunoprecipitated; KD, kinase dead; PAK, p21‐activated kinase; WB, western blot; WT, wild type; Δ50=PAK4a(51–591). Yohendran Baskaran et al. EMBO Rep. 2012;13:653-659 © as stated in the article, figure or figure legend