Volume 52, Issue 2, Pages (August 2007)

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Volume 52, Issue 2, Pages 470-477 (August 2007) Glutathione S-Transferase-P1 Expression Correlates with Increased Antioxidant Capacity in Transitional Cell Carcinoma of the Urinary Bladder Ana Savic-Radojevic, Jasmina Mimic-Oka, Marija Pljesa-Ercegovac, Marija Opacic, Dejan Dragicevic, Tamara Kravic, Milan Djokic, Sava Micic, Tatjana Simic European Urology Volume 52, Issue 2, Pages 470-477 (August 2007) DOI: 10.1016/j.eururo.2007.01.046 Copyright © 2007 European Association of Urology Terms and Conditions

Fig. 1 γ-Glutamylcysteine synthetase (γ-GCS) and glutathione reductase (GR) activities in normal uroepithelium and TCC. γ-GCS and GR activities are presented as individual values in non-tumour and tumour tissue (A and B, respectively) as well as a percentage of activities (mean±SD) measured in normal uroepithelium in groups of patients divided according to tumour grade and invasiveness (C and D, respectively). *p<0.05. European Urology 2007 52, 470-477DOI: (10.1016/j.eururo.2007.01.046) Copyright © 2007 European Association of Urology Terms and Conditions

Fig. 2 Superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities in normal uroepithelium and TCC. SOD and GPX activities are presented as individual values in non-tumour and tumour tissue (A and B, respectively) as well as a percentage of activities (mean±SD) measured in normal uroepithelium in groups of patients divided according to tumour grade and invasiveness (C and D, respectively). European Urology 2007 52, 470-477DOI: (10.1016/j.eururo.2007.01.046) Copyright © 2007 European Association of Urology Terms and Conditions

Fig. 3 Immunoblot analysis of glutathione S-transferase P1 (GSTP1) in normal bladder uroepithelium (U) and tumour tissue (T) of patients with TCC. Proteins (60μg) were resolved by SDS/PAGE and analyzed with antisera against class π. Lanes M1 (500ng), M2 (250ng), and M3 (50ng) contain purified human placental GSTP1. European Urology 2007 52, 470-477DOI: (10.1016/j.eururo.2007.01.046) Copyright © 2007 European Association of Urology Terms and Conditions

Fig. 4 Correlation between GSTP1 expression and GSH content (A), γ-GCS (B), and GR activities (C) in TCC. European Urology 2007 52, 470-477DOI: (10.1016/j.eururo.2007.01.046) Copyright © 2007 European Association of Urology Terms and Conditions